1887

Chapter 5.14 : Preparation of Routine Media and Reagents Used in Antimicrobial Susceptibility Testing

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Preview this chapter:
Zoom in
Zoomout

Preparation of Routine Media and Reagents Used in Antimicrobial Susceptibility Testing, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555817435/9781555815271_Chap5_14-1.gif /docserver/preview/fulltext/10.1128/9781555817435/9781555815271_Chap5_14-2.gif

Abstract:

McFarland turbidity standards are used to standardize the approximate number of bacteria in a liquid suspension by visually comparing the turbidity of a test suspension with the turbidity of a McFarland standard. McFarland standards are prepared by adding barium chloride to sulfuric acid to obtain a barium sulfate precipitate. By adjusting the volumes of these two reagents, standards of varying degrees of turbidity can be prepared to represent several different concentrations of bacteria. The standard most commonly used in the clinical microbiology laboratory for routine antimicrobial susceptibility tests ( ) is 0.5, which represents 1.5 × 10 (generally, range is 1.0 × 10 to 2.0 × 10) bacteria/ml. McFarland standards are commercially available from several sources. As an alternative to the traditional barium sulfate standards, McFarland standards prepared from latex particles have recently become commercially available.

Citation: Garcia L. 2010. Preparation of Routine Media and Reagents Used in Antimicrobial Susceptibility Testing, p 181-201. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.14
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

Figures

Image of Untitled
Untitled

Citation: Garcia L. 2010. Preparation of Routine Media and Reagents Used in Antimicrobial Susceptibility Testing, p 181-201. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.14
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Untitled
Untitled

Citation: Garcia L. 2010. Preparation of Routine Media and Reagents Used in Antimicrobial Susceptibility Testing, p 181-201. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.14
Permissions and Reprints Request Permissions
Download as Powerpoint

References

/content/book/10.1128/9781555817435.chap5.14
1.NCCLS. 2003. Performance Standards for Antimicrobial Disk Susceptibility Tests, 8th ed. Approved standard M2-A8. NCCLS, Wayne, Pa.
2.NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.
3. Barry, A. L. 1976. The Antimicrobic Susceptibility Test: Principles and Practices, p. 7274. Lea and Febiger, Philadelphia, Pa.
4. McFarland, J. 1907. Nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. JAMA 14:11761178.
5. Washington, J. A.,, E. Warren,, and A. G. Karlson. 1972. Stability of barium sulfate turbidity standards. Appl. Microbiol. 24:1013.
1.NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.
2. Shungu, D. L., 1996. Chemical and physical properties of antibiotics: preparation and control of antibiotic susceptibility disks and other devices containing antibiotics, p. 766792. In V. Lorian (ed)., Antibiotics in Laboratory Medicine, 4th ed. The Williams and Wilkins Co., Baltimore, Md.
1. Costilow, R. N., 1981. Biophysical factors in growth, p. 6769. In P. Gerhardt (ed.), Manual of Methods for General Bacteriology. American Society for Microbiology, Washington, D.C..
1.NCCLS. 2003. Performance Standards for Antimicrobial Disk Susceptibility Tests, 8th ed. Approved standard M2-A8. NCCLS, Wayne, Pa.
2.NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.
3.NCCLS. 1996. Protocols for Evaluating Dehydrated Mueller-Hinton Agar. Approved standard M6-A. NCCLS, Wayne, Pa.
4.NCCLS. 2001. Evaluation of Lots of Mueller-Hinton Broth for Antimicrobial Susceptibility Testing. Proposed guideline M32-P. NCCLS, Wayne, Pa.
13. NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.
1.NCCLS. 2003. Performance Standards for Antimicrobial Disk Susceptibility Tests, 8th ed. Approved standard M2-A8. NCCLS, Wayne, Pa.
2.NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.

Tables

Generic image for table
Untitled

Citation: Garcia L. 2010. Preparation of Routine Media and Reagents Used in Antimicrobial Susceptibility Testing, p 181-201. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.14
Generic image for table
Untitled

Citation: Garcia L. 2010. Preparation of Routine Media and Reagents Used in Antimicrobial Susceptibility Testing, p 181-201. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.14
Generic image for table
Untitled

Citation: Garcia L. 2010. Preparation of Routine Media and Reagents Used in Antimicrobial Susceptibility Testing, p 181-201. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.14
Generic image for table

Notes and QC parameters for media

For additional information, see QC information in procedures 5.1 and 5.2, and also see procedure 5.13.

Agar depth is critical for disk testing; must be 3 to 5 mm.

MRSA, methicillin-resistant .

Citation: Garcia L. 2010. Preparation of Routine Media and Reagents Used in Antimicrobial Susceptibility Testing, p 181-201. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.14

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error