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Chapter 5.17 : Evaluating Antimicrobial Susceptibility Test Systems

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Abstract:

A wide variety of antimicrobial susceptibility test systems are available to microbiologists today. This procedure provides guidance on evaluating and choosing a system or method appropriate for your laboratory. The primary focus should be on determination of the systems' capabilities, assessment of costs and work flow, and examination of performance data.

Citation: Garcia L. 2010. Evaluating Antimicrobial Susceptibility Test Systems, p 229-239. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.17
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References

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1.Food and Drug Administration. 1991. Review Criteria for Assessment of Antimicrobial Susceptibility Devices, revised version. Food and Drug Administration, Washington, D.C. (2000 draft document available for review and comment at http://www.fda.gov/cdrh/ode/631.html.).
2.NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.
3. Ferraro, M. J.,, and J. H. Jorgensen,. 2003. Susceptibility testing instrumentation and computerized expert systems for data analysis and interpretation, p. 208217. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C..
4. Jorgensen, J. H. 1993. Selection criteria for an antimicrobial susceptibility testing system. J. Clin. Microbiol. 31:28412844.
5. Jorgensen, J. H.,, and M. J. Ferraro. 1998. Antimicrobial susceptibility testing: general principles and contemporary practices. Clin. Infect. Dis. 26:973980.
6. Jorgensen, J. H.,, and M. J. Ferraro. 2000. Antimicrobial susceptibility testing: special needs for fastidious organisms and difficult-to-detect resistance mechanisms. Clin. Infect. Dis. 30:799808.
7. Livermore, D. M.,, T. G. Winstanley,, and K. P. Shannon. 2001. Interpretive reading: recognizing the unusual and inferring resistance mechanisms from resistance phenotypes. J. Antimicrob. Chemother. 48(S1):87102.

Tables

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Susceptibility category errors

Abbreviations: R, resistant; S, susceptible; I, intermediate.

Citation: Garcia L. 2010. Evaluating Antimicrobial Susceptibility Test Systems, p 229-239. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.17
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Cefazolin: XYZ system compared with NCCLS reference microdilution MIC method

As determined by reference method. Abbreviations: S, susceptible; I, intermediate; R, resistant.

Calculations include only isolates susceptible by reference method.

Calculations include only isolates resistant by reference method.

NA, not applicable.

Citation: Garcia L. 2010. Evaluating Antimicrobial Susceptibility Test Systems, p 229-239. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.17
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Correlation table for XYZ system compared with NCCLS reference microdilution MIC method

EA, essential agreement; CA, category agreement.

Citation: Garcia L. 2010. Evaluating Antimicrobial Susceptibility Test Systems, p 229-239. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.17

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