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Chapter 5.3 : Beta-Lactamase Tests

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Beta-Lactamase Tests, Page 1 of 2

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Abstract:

Beta-lactamases are enzymes produced by many clinically significant bacteria and are major mediators of bacterial resistance to beta-lactam agents. Routine beta-lactamase tests are based on visual detection of the end products of beta-lactamase hydrolysis, which is demonstrated with a colorimetric reaction. These tests primarily include the chromogenic cephalosporin method, the acidimetric method, and the iodometric method and are summarized in Appendix 5.3-1 . Not every method is satisfactory for detecting beta-lactamase produced by all of the bacteria for which the test is useful.

Citation: Garcia L. 2010. Beta-Lactamase Tests, p 42-47. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.3
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Citation: Garcia L. 2010. Beta-Lactamase Tests, p 42-47. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.3
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References

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1.NCCLS. 2003. Performance Standards for Antimicrobial Disk Susceptibility, 8th ed.Approved standard M2-A8. NCCLS, Wayne, Pa.
2. NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.
3. Becton Dickinson Microbiology Systems. 1996. BBL paper disks for the detection of beta-lactamase enzymes. Product 88-0973-1. Becton Dickinson Microbiology Systems, Cockeysville, Md.
4. Livermore, D. M.,, and D. F. Brown. 2001. Detection of beta-lactamase-mediated resistance. J. Antimicrob. Chemother. 48:5964.
5. Swenson, J. M.,, J. A. Hindler,, and J. H. Jorgensen,. 2003. Special phenotypic methods for detecting antibacterial resistance, p. 11781195. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C.

Tables

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Summary of beta-lactamase testing methods

Citation: Garcia L. 2010. Beta-Lactamase Tests, p 42-47. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.3
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Method(s) generally satisfactory for various organisms

Citation: Garcia L. 2010. Beta-Lactamase Tests, p 42-47. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.3

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