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Chapter 5.4 : Oxacillin Salt-Agar Screen Test To Detect Oxacillin (Methicillin)-Resistant

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Abstract:

A standard number of bacteria is inoculated onto Mueller-Hinton agar (MHA) containing 6 µg of oxacillin per ml and 4% NaCl. Following overnight incubation, the appearance of growth indicates that the isolate is resistant to oxacillin and other penicillinase-stable penicillins (methicillin, nafcillin, cloxacillin, and dicloxacillin) ( ).

Citation: Garcia L. 2010. Oxacillin Salt-Agar Screen Test To Detect Oxacillin (Methicillin)-Resistant , p 48-51. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.4
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References

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1.NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria ThatGrow Aerobically, 6th ed.Approved standard.M7-A6. NCCLS, Wayne, Pa.
2. Swenson, J. M.,, P. P. Williams,, G. Killgore,, C. M. O'Hara,, and F. C. Tenover. 2001. Performance of eight methods, including two new rapid methods, for detection of oxacillin resistance in a challenge set of Staphylococcus aureus organisms. J. Clin. Microbiol. 39:37853788.
3. Jorgensen, J. H., 2003. Susceptibility test methods:dilution and disk diffusion methods, p. 11081127. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken(ed.), Manual of Clinical Microbiology, 8th ed. ASM Press,Washington, D.C.
4. Swenson, J. M.,, J. A. Hindler,, and J. H. Jorgensen,. 2003. Special phenotypic methods for detectingantimicrobial resistance, p. 11781195. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C.
5. Swenson, J. M.,, J. Spargo,, F. C. Tenover, andM. J. Ferraro. 2001. Optimal inoculation methodsand quality control for the NCCLS oxacillinagar screen test for detection of oxacillin resistance in Staphylococcus aureus. J. Clin. Microbiol.39:37813784.

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Oxacillin Agar Screen Test QC

Citation: Garcia L. 2010. Oxacillin Salt-Agar Screen Test To Detect Oxacillin (Methicillin)-Resistant , p 48-51. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.4

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