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Chapter 5.5 : Screen Tests To Detect High-Level Aminoglycoside Resistance in spp.

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Screen Tests To Detect High-Level Aminoglycoside Resistance in spp., Page 1 of 2

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Abstract:

High-level aminoglycoside resistance (HLAR) in enterococci is most commonly detected by assessing growth at high concentations of gentamicin (500 µg/ml) and sometimes streptomycin (1,000 µg/ml in broth and 2,000 µg/ml in agar) ( ). Strains that show HLAR to gentamicin will not be synergistically killed with combinations of cell wall-active drugs (generally ampicillin, penicillin, or vancomycin) plus gentamicin. HLAR to gentamicin also means HLAR to tobramycin, netilmicin, amikacin, and kanamycin. HLAR to streptomycin equals resistance to combinations of cell wall-active drugs and streptomycin. See additional information in Appendix 5.5-1 . The agar screen test is described in detail here, and reference is made to the disk diffusion and the broth microdilution MIC test procedures for detecting HLAR.

Citation: Garcia L. 2010. Screen Tests To Detect High-Level Aminoglycoside Resistance in spp., p 52-56. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.5
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References

/content/book/10.1128/9781555817435.chap5.5
1.NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.
2.NCCLS. 2003. Performance Standards for Antimicrobial Disk Susceptibility Tests, 8th ed. Approved standard M2-A8. NCCLS, Wayne, Pa.
3. Chow, J. W. 2000. Aminoglycoside resistance in enterococci. Clin. Infect. Dis. 31:586589.
4. > Swenson, J. M.,, J. A. Hindler,, and J. H. Jorgensen,. 2003. Special phenotypic methods for detecting antibacterial resistance, p. 11781195. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C.

Tables

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Aminoglycoside-modifying enzymes that confer HLAR on spp.

Y, modifies the activity of the respective aminoglycoside; no synergy achievable with a cell wall-active agent. N, does not modify the activity of the respective aminoglycoside; synergy achievable.

Found in virtually all organisms.

Citation: Garcia L. 2010. Screen Tests To Detect High-Level Aminoglycoside Resistance in spp., p 52-56. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.5
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HLAR Agar Screen Test QC

Citation: Garcia L. 2010. Screen Tests To Detect High-Level Aminoglycoside Resistance in spp., p 52-56. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.5

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