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Chapter 5.7 : Broth Microdilution MIC Test for Anaerobic Bacteria

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Abstract:

The broth microdilution MIC method can be used to measure (semiquantitatively) the in vitro activity of an antimicrobial agent against an anaerobic bacterial isolate. This procedure is very similar to the broth microdilution method used for aerobic bacteria. Various concentrations of antimicrobial agents are dispensed into a multiwell plastic tray and inoculated with a test isolate. After 48 h of incubation at 35°C in an anaerobic environment, the MIC is determined by observing the lowest concentration of an antimicrobial agent which will inhibit visible growth of the test bacterium. MICs obtained are interpreted as susceptible, intermediate, or resistant, based on criteria defined by the NCCLS ( ).

Citation: Garcia L. 2010. Broth Microdilution MIC Test for Anaerobic Bacteria, p 61-68. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.7
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References

/content/book/10.1128/9781555817435.chap5.7
1.NCCLS. 2001. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Anaerobically. Approved standard M11-A5. NCCLS, Wayne, Pa.
2. Citron, D. M.,, and D. W. Hecht,. 2003. Susceptibility test methods: anaerobic bacteria, p. 11411148. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C.
3. Finegold, S. M.,, and W. L. George. 1989. Anaerobic Infections in Humans. Academic Press, Inc., San Diego, Calif.
4. Holdeman, L. V.,, E. P. Cato,, and W. E. C. Moore(ed.). 1977. Anaerobe Laboratory Manual, 4th ed. Virginia Polytechnic Institute and State University, Blacksburg.
5. Jousimies-Somer, H. R.,, P. Summanen,, D. M. Citron,, E. J. Baron,, H. M. Wexler,, and S. M. Finegold. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, Calif.
1. Jousimies-Somer, H. R.,, P. Summanen,, D.M. Citron,, E. J. Baron,, H. M. Wexler,, and S. M. Finegold. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual,6th ed. Star Publishing Co., Belmont, Calif.
2.NCCLS. 2001. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Anaerobically. Approved standard M11-A5. NCCLS, Wayne, Pa.

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Citation: Garcia L. 2010. Broth Microdilution MIC Test for Anaerobic Bacteria, p 61-68. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.7
Generic image for table
Untitled

Citation: Garcia L. 2010. Broth Microdilution MIC Test for Anaerobic Bacteria, p 61-68. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.7

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