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Chapter 5.9 : Agar Dilution MIC Test for Anaerobic Bacteria
The anaerobe agar dilution MIC method is a semiquantitative method for the determination of in vitro activity of an antimicrobial agent against anaerobic bacterial isolates. A series of agar plates, each containing a unique concentration of an antimicrobial agent, are inoculated with up to 36 isolates. After 48 h of incubation at 35°C in an anaerobic environment, the MIC is determined by observing the lowest concentration of antimicrobial agent which will inhibit visible growth of the test isolate. MICs obtained are interpreted as susceptible, intermediate, or resistant based on criteria defined by the NCCLS ( 2 ).