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Chapter 7.1 : General Procedures

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Abstract:

All specimens and cultures handled in the mycobacteriology laboratory must be processed under an appropriate biological safety cabinet (BSC). Personal protective clothing (gowns, gloves, and respirators) is required for processing, smear preparation, and culturing for mycobacteria. Centrifuges must be equipped with aerosol-free safety canisters. Refer to CDC publications for detailed safety recommendations ( ).

Citation: Garcia L. 2010. General Procedures, p 280-292. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.1
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References

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1.Centers for Disease Control and Prevention. 1999. Biosafety in Microbiological and Biomedical Laboratories, 4th ed. Centers for Disease Control and Prevention, Atlanta, Ga.
2. Garner, J. 1996. CDC Guideline for Isolation Precautions in Hospitals, Hospital Infection Control Advisory Committee. Centers for Disease Control and Prevention, Atlanta, Ga.
3. Hawkins, J. E.,, R. C. Good,, G. P. Kubica,, P. R. J. Gangadharam,, H. M. Guft,, D. D. Stottmeier,, H. M. Sommers,, and L. G. Wayne. 1983. Levels of laboratory services for mycobacterial diseases: official statement of the American Thoracic Society. Am. Rev. Respir. Dis. 128:213.
4. Richmond, J. Y. R.,, C. Knudsen,, and R. C. Good. 1996. Biosafety in the clinical microbiology laboratory. Clin. Lab. Med. 16:527550.
5. Salfinger, M. 1995. Role of the laboratory in evaluating patients with mycobacterial disease. Clin. Microbiol. Newsl. 17:108109.
1. Cooper, H. J.,, and N. Uyei. 1930. Oxalic acid as a reagent for isolating tubercle bacilli and a study of the growth of acid-fast nonpathogens on different mediums with their reaction to chemical reagents. J. Lab. Clin. Med. 15:348369.
2. Della-Latta, P. 1996. Workflow and optimal protocols for laboratories in industrialized countries. Clin. Lab. Med. 16:677695.
3. Garner, J. 1996. CDC Guideline for Isolation Precautions in Hospitals, Hospital Infection Control Advisory Committee. Centers for Disease Control and Prevention, Atlanta, Ga..
4. Good, R. C.,, V. Silcox,, and J. O. Kilburn,. 1981. Tuberculosis and other mycobacterioses, p. 675703. In A. Balows, and W. J. Hausler (ed.), Diagnostic Procedures for Bacterial, Mycotic and Parasitic Infections, 6th ed. American Public Health Association, Washington, D.C..
5. Gullans, C. R., Sr., 1992. Preparation of specimens for mycobacterial culture, p. 3.3.13.3.6 In H. D. Isenberg (ed.), Clinical Microbiology Procedures Handbook, vol. 1. American Society for Microbiology, Washington, D.C..
6. Kent, P. T.,, and G. P. Kubica. 1985. Public Health Mycobacteriology. A Guide for the Level III Laboratory. U.S. Department of Health and Human Services, Centers for Disease Control, Atlanta, Ga..
7. Kubica, G. P.,, W. E. Dye,, M. L. Cohn,, and G. Middlebrook. 1963. Sputum digestion and decontamination with N-acetyl-L-cysteine-so-dium hydroxide for culture of mycobacteria. Am. Rev. Respir. Dis. 87:775779.
8. Patterson, R. A.,, T. L. Thompson,, and D. H. Larsen. 1956. The use of Zephiran in the isolation of M. tuberculosis. Am. Rev. Tuberc. 74:284288.
9. Pfyffer, G. E.,, B. A. Brown-Elliott,, and R. J. Wallace, Jr., 2003. Mycobacterium, p. 532584. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C..
10. Smithwich, R. W. 1976. Laboratory Manual for Acid-Fast Microscopy, 2nd ed. Center for Disease Control, Atlanta, Ga..
16. Tenover, F. C.,, J. T. Crawford,, R. E. Huebner,, L. J. Geiter,, C. R. Horsburgh, Jr., and R. C. Good. 1993. The resurgence of tuberculosis: is your laboratory ready? J. Clin. Microbiol. 31:767770.
1.Centers for Disease Control. 1989. A stra-tegic plan for the elimination of tuberculosis in the United States. Morb. Mortal. Wkly. Rep. 38(Suppl. S-3):125.
2.Difco Laboratories. 1984. Difco Manual. De-hydrated Culture Media and Reagents for Microbiology, 10th ed. Difco Laboratories, Detroit, Mich..
3. Garner, J. 1996. CDC Guideline for Isolation Precautions in Hospitals, Hospital Infection Control Advisory Committee. Centers for Disease Control and Prevention, Atlanta, Ga..
4. Gullans, C. R., Sr., 1992. Digestion-decontamination procedures, p. 3.4.13.4.14. In H. D. Isenberg (ed.), Clinical Microbiology Procedures Handbook, vol. 1. American Society for Microbiology, Washington, D.C..
5. Gullans, C. R., Sr., 1992. Preparation of specimens for mycobacterial culture, p. 3.3.13.3.6. In H. D. Isenberg (ed.), Clinical Microbiology Procedures Handbook, vol. 1. American Society for Microbiology, Washington, D.C..
6. Kent, P. T.,, and G. P. Kubica. 1985. Public Health Mycobacteriology. A Guide for the Level III Laboratory. U.S. Department of Health and Human Services, Centers for Disease Control, Atlanta, Ga..
7. Pfyffer, G. E.,, B. A. Brown-Elliott,, and R. J. Wallace, Jr., 2003. Mycobacterium, p. 532584. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C..
8. Smithwich, R. W. 1976. Laboratory Manual for Acid-Fast Microscopy, 2nd ed. Center for Disease Control, Atlanta, Ga..
1.Centers for Disease Control. 1989. A strategic plan for the elimination of tuberculosis in the United States. Morb. Mortal. Wkly. Rep. 38(Suppl. S-3):125.
2. Della-Latta, P. 1996. Workflow and optimal protocols for laboratories in industrialized countries. Clin. Lab. Med. 16:677695.
3. Tenover, F. C.,, J. T. Crawford,, R. E. Huebner,, L. J. Geiter,, C. R. Horsburgh, Jr.,, and R. C. Good. 1993. The resurgence of tuberculosis: is your laboratory ready? J. Clin. Microbiol. 31:767770.

Tables

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Untitled

Citation: Garcia L. 2010. General Procedures, p 280-292. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.1
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Table 7.1.2–A1

Preparation of NALC-NaOH digestant-decontaminant

Citation: Garcia L. 2010. General Procedures, p 280-292. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.1
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Turnaround time expectations in typical mycobacteriology laboratory

Citation: Garcia L. 2010. General Procedures, p 280-292. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.1

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