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Chapter 7.2 : Acid-Fast Stains

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Acid-Fast Stains, Page 1 of 2

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Abstract:

Mycobacteria are difficult to stain due to the presence of large amounts of lipid (mycolic acid) in the cell wall; hence, the use of the traditional Gram stain is of little to no value since the dyes do not usually permeate the mycobacterial cell walls. Instead, the most widely used methods to determine acid-fastness in a clinical specimen are the carbol fuchsin stains (Kin-youn or Ziehl-Neelsen) and the fluorochrome stain (e.g., auramine O or r auramine-rhodamine). The fluorochrome stains are recommended for the examination of clinical specimens because of their increased sensitivity and speed, since they may be examined at a lower magnification than the carbol fuchsin stain ( ).

Citation: Garcia L. 2010. Acid-Fast Stains, p 293-296. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.2
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References

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1.Centers for Disease Control and Prevention. 1999. Biosafety in Microbiological and Biomedical Laboratories, 4th ed. Centers for Disease Control and Prevention, Atlanta, Ga..
2. Daniel, T. M. 1990.The rapid diagnosis of tuberculosis: a selective review. J. Lab. Med. 116:277282.
3. Della-Latta, P.,, and I. Weitzman,. 1998. Acid-fast stain procedures, p. 176178. In H. D. Isenberg (ed.), Essential Procedures for Clinical Microbiology. ASM Press, Washington, D.C..
4. Ebersole, L. L., 1992. Acid-fast stain procedures, p. 3.5.13.5.11. In H. D. Isenberg (ed.), Clinical Microbiology Procedures Handbook, vol. 1. American Society for Microbiology, Washington, D.C..
5. Kent, P. T.,, and G. P. Kubica. 1985. Public Health Mycobacteriology. A Guide for the Level III Laboratory, p. 5768. U.S. Department of Health and Human Services, Centers for Disease Control, Atlanta, Ga..
6. Pfyffer, G. E.,, B. A. Brown-Elliott,, and R. J. Wallace, Jr., 2003. Mycobacterium, p. 532584. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C..
7. Salfinger, M.,, and G. E. Pfyffer. 1994. The new diagnostic mycobacteriology laboratory. Eur. J. Clin. Microbiol. Infect. Dis. 13:961979.
8. Yeager, H. J., Jr.,, J. Lacy,, L. Smith,, and C. LeMaistre. 1967. Quantitative studies of mycobacterial populations in sputum and saliva. Am. Rev. Respir. Dis. 95:9981004.

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Citation: Garcia L. 2010. Acid-Fast Stains, p 293-296. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.2

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