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Chapter 7.3 : Solid Media for Isolation

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Abstract:

Both liquid and solid media are recommended for optimal recovery of mycobacteria. The advantage of solid media (tubed or in plates) is that they enable detection of mixed cultures and contaminants. Egg-based and agar-based media may be used. The main advantage of an egg-based medium is that it supports the growth of most mycobacteria and permits niacin testing. However, contamination occurs more easily involving the total surface of the medium. The main advantages of agar-based media are less contamination and earlier and easier visibility of colonial morphology. Colonial morphology aids in the identification of mycobacteria. Use of both nonselective and selective media is needed for isolation, the latter containing one or more antimicrobial agents to prevent overgrowth by contaminating bacteria or fungi ( ).

Citation: Garcia L. 2010. Solid Media for Isolation, p 297-300. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.3
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References

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1. Kent, P. T.,, and G. P. Kubica. 1985. Public Health Mycobacteriology. A Guide for the Level III Laboratory. U.S. Department of Health and Human Services, Centers for Disease Control, Atlanta, Ga..
2. Lambi, E. A., 1992. Medium selection and incubation for the isolation of mycobacteria, p. 3.6.1.3.6.8.In H. D. Isenberg (ed.), Clinical Microbiology Procedures Handbook, vol. 1. American Society for Microbiology, Washington, D.C..
3.NCCLS. 1990. Quality Assurance for Commercially Prepared Microbiological Culture Media. NCCLS document M-22A, vol. 16, no. 16. NCCLS, Villanova, Pa..
4. Pfyffer, G. E.,, B. A. Brown-Elliott,, and R. J. Wallace, Jr., 2003. Mycobacterium, p. 532584. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C..
5. Silcox, V., 1992. Identification of mycobacteria, p. 3.11.13.11.11. In H. D. Isenberg (ed.), Clinical Microbiology Procedures Handbook, vol. 1. American Society for Microbiology, Washington, D.C..
6. Whittier, S.,, R. L. Hopfer,, M. R. Knowles,, and P. H. Gilligan. 1993. Improved recovery of mycobacteria from respiratory secretions of patients with cystic fibrosis. J. Clin. Microbiol. 31:861864.

Tables

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Table 7.3–1

Media commonly available for recovery of mycobacteria ( )

Reproduced and modified from 1984. Mycobacterial diseases. J. B. Henry (ed.), 17th ed. The W. B. Saunders Co., Philadelphia, Pa., with permission of Elsevier.

Citation: Garcia L. 2010. Solid Media for Isolation, p 297-300. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.3
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Citation: Garcia L. 2010. Solid Media for Isolation, p 297-300. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.3

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