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Chapter 7.5 : Septi-Chek AFB Biphasic Medium
The BD BBL Septi-Chek AFB mycobacterial culture system (Becton Dickinson Microbiology Systems, Cockeysville, Md.) is a biphasic medium with a self-contained CO2 environment. It consists of Middlebrook 7H9 broth and a slide of Middlebrook 7H11, modified egg, and chocolate media. The system offers sensitivity comparable to those of conventional agar and broth methods ( 1 , 4 ). The inclusion of CHOC in this system is advantageous because it detects contaminants and when incubated at 30°C, Mycobacterium haemophilum can be detected.