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Chapter 7.7 : Susceptibility Tests by Modified Agar Proportion

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Abstract:

Drug susceptibility testing should be performedon all initial complex isolates and in relapse or retreatment cases and when primary drug resistance is suspected. The modified agar proportion method is used to determine the susceptibility of slow-growing mycobacteria (e.g., complex, ), either directly from decontaminated acid-fast smear-positive sputum or indirectly from a pure culture. The agar medium is contained in quadrant plates; one is the control without antimicrobial agents and the other quadrants contain antimicrobial agents. The primary drugs, i.e., isoniazid (INH),ethambutol, and rifampin, are commonly selected for testing; however, pyrazinamide cannot be tested by this method ( ).

Citation: Garcia L. 2010. Susceptibility Tests by Modified Agar Proportion, p 347-350. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.7
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References

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1.Centers for Disease Control and Prevention. 1993. Initial therapy for tuberculosis in the era of multidrug resistance. Recommendations of the Advisory Council for the Elimination of Tuberculosis. Morb. Mortal. Wkly. Rep. 42(RR-7):18.
2. Kent, P. T.,, and G. P. Kubica. 1985. Public Health Mycobacteriology. A Guide for the Level III Laboratory. U.S. Department of Health and Human Services, Centers for Disease Control, Atlanta, Ga..
3.NCCLS. 2000. Susceptibility Testing of Mycobacteria, Nocardia, and Other Aerobic Actinomycetes, 2nd ed., vol. 20, no. 26. Tentative standard M24-T2. NCCLS, Wayne, Pa..
4. Pfyffer, G. E.,, B. A. Brown-Elliott,, and R. J. Wallace, Jr., 2003. Mycobacterium, p. 532584. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C..
5. Wayne, L. G.,, and I. Krasnow. 1966 Preparation of tuberculosis susceptibility testing medium by means of impregnated discs. Am. J. Clin. Pathol. 45:769771.

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Citation: Garcia L. 2010. Susceptibility Tests by Modified Agar Proportion, p 347-350. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.7
Generic image for table
Untitled

Citation: Garcia L. 2010. Susceptibility Tests by Modified Agar Proportion, p 347-350. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.7
Generic image for table
Untitled

Citation: Garcia L. 2010. Susceptibility Tests by Modified Agar Proportion, p 347-350. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.7

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