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Chapter 8.10 : Antifungal Susceptibility Testing
Until recently, antifungal susceptibility testing (AFST) has lagged behind its antibacterial counterpart. A number of important achievements helped propel the antifungal susceptibility field. These achievements include publication of the approved reference method for broth dilution AFST of yeast, document M27-A3 of the Clinical and Laboratory Standards Institute (CLSI; formerly National Committee for Clinical Laboratory Standards [NCCLS]) ( 1 , 2 ); publication of interpretive breakpoints using this method ( 10 ); the recommended use of AFST during selection of therapy of patients with candidemia and hematogenously disseminated candidiasis as outlined by the published guidelines for the treatment of candidiasis ( 6 ); publication of a reference method for broth dilution AFST of conidium-forming filamentous fungi, CLSI document M38-A2 ( 3 ); and recent development and publication of AFST methods for dermatophytes ( 3 ). The procedures described below are based on CLSI documents M27-A3, M27-S3, and M38-A2.