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Chapter 9.3 : Macroscopic and Microscopic examination of Fecal Specimens

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Abstract:

The age of fresh fecal specimens is an important factor in the diagnosis of parasitic infections ( ). The date and time of passage must be provided for each specimen submitted to the laboratory. The physical characteristics of a fresh fecal specimen may aid in determining what types of organisms may be present ( ). Fecal specimens are described as formed, semiformed, soft, loose, or watery. Loose or watery specimens may contain trophozoites, whereas formed or semiformed specimens are more likely to contain cyst stages. Helminth eggs or larvae may be found in any type of specimen but are more difficult to find in liquid specimens because of the dilution factor. One can also see if blood and/or mucus is present, although if present, neither one necessarily indicates a parasitic infection. When the fresh specimen is examined visually in the collection container, adult pinworms and tapeworm proglottids may also be seen.

Citation: Garcia L. 2010. Macroscopic and Microscopic examination of Fecal Specimens, p 559-595. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.3
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Figures

Image of Figure 9.3.2–1
Figure 9.3.2–1

Ocular micrometer, top scale; stage micrometer, bottom scale (from , 5th ed., 2007, ASM Press, Washington, DC).

Citation: Garcia L. 2010. Macroscopic and Microscopic examination of Fecal Specimens, p 559-595. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.3
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Image of Figure 9.3.4–1
Figure 9.3.4–1

Diagram of specimen after centrifugation (sedimentation procedure).

Citation: Garcia L. 2010. Macroscopic and Microscopic examination of Fecal Specimens, p 559-595. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.3
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Image of Figure 9.3.4–A1
Figure 9.3.4–A1

Countertop workstation that automates the microscopic analysis of fecal concentrates (DiaSys Corp.) (from , , 5th ed., 2007, ASM Press, Washington, DC).

Citation: Garcia L. 2010. Macroscopic and Microscopic examination of Fecal Specimens, p 559-595. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.3
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Image of Figure 9.3.4–A2
Figure 9.3.4–A2

Dual-flow-cell Optical Slide Assembly (DiaSys Corp.) that fits into the stage clips of any standard upright microscope (from , , 5th ed., 2007, ASM Press, Washington, DC).

Citation: Garcia L. 2010. Macroscopic and Microscopic examination of Fecal Specimens, p 559-595. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.3
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Image of Figure 9.3.5–1
Figure 9.3.5–1

Diagram of specimen after centrifugation (flotation procedure).

Citation: Garcia L. 2010. Macroscopic and Microscopic examination of Fecal Specimens, p 559-595. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.3
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References

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Tables

Generic image for table

Diagnostic characteristics of organisms in wet mounts

Citation: Garcia L. 2010. Macroscopic and Microscopic examination of Fecal Specimens, p 559-595. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.3

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