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Chapter 9.7 : Sputum, Aspirates, and Biopsy Material

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Citation: Garcia L. 2010. Sputum, Aspirates, and Biopsy Material, p 680-695. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.7
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References

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1. del Rio, C.,, J. Guarner,, E. G. Honig,, and B. A. Slade. 1988. Sputum examination in the diagnosis of Pneumocystis carinii pneumonia in the acquired immunodeficiency syndrome. Arch. Pathol. Lab. Med. 112:12291232.
2. Garcia, L. S. 2007. Diagnostic Medical Parasitology, 5th ed. ASM Press, Washington, DC.
3. Melvin, D. M.,, and M. M. Brooke. 1975. Laboratory Procedures for Diagnosis of Intestinal Parasites Department of Health, Education and Welfare publication no. (CDC) 76-8282. U.S. Government Printing Office, Washington, DC.
1. Brinn, N. T. 1983.Rapid metallic histological staining using the microwave oven. J. Histotechnol. 6: 125129.
2. Derouin, F.,, C. Sarfati,, B. Beauvais,, M. Il-iou,, L. Dehen,, and M. Lariviere. 1989. Laboratory diagnosis of pulmonary toxoplasmosis in patients with acquired immunodeficiency syndrome. J. Clin. Microbiol .27:16611663.
3. Garcia, L. S. 2007. Diagnostic Medical Parasitology, 5th ed. ASM Press, Washington, DC.
4. Haley, L. D.,, and P. G. Standard. 1973. Laboratory Methods in Medical Mycology, p. 100. Center for Disease Control, Atlanta, GA.
5. Martin, W. J., II,, T. F. Smith,, D. R. Sanderson,, W. M. Brutinel,, F. R. Cockerill III,, and W. W. Douglas. 1989. Role of bronchoalveolar lavage in the assessment of opportunistic pulmonary infection: utility and complication. Mayo Clin. Proc. 62:549557.
6. McQuay, R. M. 1956. Charcoal medium for growth and maintenance of large and small races of Entamoeba histolytica. Am. J. Clin. Pathol. 26:11371138.
1. Arrowood, M. J.,, and C. R. Sterling. 1989. Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection. J. Clin. Microbiol. 27:14901495.
2. Cali, A.,, and R. L. Owen. 1990.Intracellular development of Enterocytozoon, a unique microsporidian found in the intestine of AIDS patients. J. Protozool. 37(2):145155.
3. Canning, E. U.,, and W. S. Hollister. 1990. Enterocytozoon bieneusi (Microspora): prevalence and pathogenicity in AIDS patients. Trans. R. Soc. Trop. Med. Hyg. 84:181186.
4.CentersforDiseaseControl. 1990. Microsporidian keratoconjunctivitis in patients with AIDS. MMWR Morb. Mortal. Wkly. Rep. 39:188189.
5. Derouin, F.,, C. I. Sarfati,, B. Beauvais,, M. Iliou,, L. Dehen,, and M. Lariviere. 1989. Laboratory diagnosis of pulmonary toxoplasmosis in patients with acquired immunodeficiency syndrome. J. Clin. Microbiol. 27:16611663.
6. Fayer, R.,, and B. L. P. Ungar. 1986. Cryptosporidium spp. and cryptosporidiosis .Microbiol. Rev. 50:458483.
7. Griffin, J. L., 1978. Pathogenic free-living amoebae, p. 507549. In J. P. Kreier (ed.), Parasitic Protozoa, vol. 15. Academic Press, Inc., New York, NY.
8. Haley, L. D.,, and P. G. Standard. 1973. Laboratory Methods in Medical Mycology, p. 100. Center for Disease Control, Atlanta, GA.
9. Jones, F. E.,, D. E. Eyles,, N. Coleman,, and C. L. Gibson. 1958. A comparison of methods for the isolation of Toxoplasma from suspected hosts. Am. J. Trop. Med. 7:531535.
10. McQuay, R. M. 1956. Charcoal medium for growth and maintenance of large and small races of Entamoeba histolytica. Am. J. Clin. Pathol. 26:11371138.
11. Novy, F. G.,, and W. J. MacNeal. 1904.The cultivation of Trypanosomabrucei. J. Infect. Dis. 1:130.
12. Rijpstra, A. C.,, E. U. Canning,, R. J. Van Ketel,, J. K. M. Eeftinck Schattenkerk,, and J. J. Laarman. 1988. Use of light microscopy to diagnose small-intestinal microsporidiosis in patients with AIDS. J. Infect. Dis. 157:827831.
13. Shadduck, J. A. 1989.Humanmicrospori-diosis and AIDS. Rev. Infect. Dis. 11:203207.
14. Sterling, C. R.,, and M. J. Arrowood. 1986. Detection of Cryptosporidium sp. Infections using a direct immunofluorescent assay. Pediatr.Infect.Dis. 5:S138S142.
15. Wheatley, W. B. 1951.A rapid staining procedure for intestinal amoebae and flagellates. Am. J. Clin. Pathol. 21:990991.
25. Bryan, R. T., 1990. Microsporidia, p. 21302134. In G. L. Mandell,, R. G. Douglas,, and J. E. Bennett (ed.), Principles and Practice of Infectious Diseases, 3rd ed. Churchill Livingstone, NewYork, NY.
26. Bryan, R. T.,, A. Cali,, R. L. Owen,, and H. C. Spencer,. 1991. Microsporidia: opportunistic pathogens in patients with AIDS, p. 126. In T. Sun (ed.), Progressin Clinical Parasitology, vol. 2. Field & Wood, Philadelphia, PA.
27. Garcia, L. S. 2007. Diagnostic Medical Parasitology, 5th ed. ASM Press, Washington, DC.
28. Ignatius, R.,, S. Henschel,, O. Liesenfeld,, U. Mansmann,, W. Schmidt,, S. Koppe,, T. Schneider,, W. Heise,, U. Futh,, E. O. Riecken,, H. Hahn,, and R. Ulrich. 1997. Comparative evaluation of modified trichrome and Uvitex 2B stains for detection of low numbers of microsporidial spores in stool specimens. J. Clin. Microbiol. 35:22662269.
29. Jelinek, T.,, S. Eichenlaub,, and T. Loscher. 1999. Sensitivity and specificity of a rapid immunochromatographic test for diagnosis of visceral leishmaniasis. Eur. J. Clin. Microbiol. Infect. Dis. 18:669670.
30. Wittner, M.,, and L. M. Weiss. 1999. The Microsporidia and Microsporidiosis. ASM Press, Washington, DC.

Tables

Generic image for table

Phosphate buffer solutions

Citation: Garcia L. 2010. Sputum, Aspirates, and Biopsy Material, p 680-695. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.7
Generic image for table
Table 9.7.3–1

Stains for identifying parasites in various tissues

: In disseminated infections with microsporidia, spores and developing stages from a number of different species could be found throughout the body. Routine histology processing and staining (H&E, silver, Gram, PAS) are used, as are Giemsa, modified trichrome stains, and optical brightening agents. However, the modified trichrome stains (chromotrope) are more commonly used and recommended for clinical specimens other than tissues, such as stool, urine, eye specimens, etc.

EM, electron microscopy.

Citation: Garcia L. 2010. Sputum, Aspirates, and Biopsy Material, p 680-695. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.7

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