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Chapter 9.7 : Sputum, Aspirates, and Biopsy Material

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Citation: Garcia L. 2010. Sputum, Aspirates, and Biopsy Material, p 680-695. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.7
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References

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1. del Rio, C.,, J. Guarner,, E. G. Honig,, and B. A. Slade. 1988. Sputum examination in the diagnosis of Pneumocystis carinii pneumonia in the acquired immunodeficiency syndrome. Arch. Pathol. Lab. Med. 112: 1229 1232.
2. Garcia, L. S. 2007. Diagnostic Medical Parasitology, 5th ed. ASM Press, Washington, DC.
3. Melvin, D. M.,, and M. M. Brooke. 1975. Laboratory Procedures for Diagnosis of Intestinal Parasites Department of Health, Education and Welfare publication no. (CDC) 76-8282. U.S. Government Printing Office, Washington, DC.
1. Brinn, N. T. 1983. Rapid metallic histological staining using the microwave oven. J. Histotechnol. 6: 125 129.
2. Derouin, F.,, C. Sarfati,, B. Beauvais,, M. Il-iou,, L. Dehen,, and M. Lariviere. 1989. Laboratory diagnosis of pulmonary toxoplasmosis in patients with acquired immunodeficiency syndrome. J. Clin. Microbiol . 27: 1661 1663.
3. Garcia, L. S. 2007. Diagnostic Medical Parasitology, 5th ed. ASM Press, Washington, DC.
4. Haley, L. D.,, and P. G. Standard. 1973. Laboratory Methods in Medical Mycology, p. 100. Center for Disease Control, Atlanta, GA.
5. Martin, W. J., II,, T. F. Smith,, D. R. Sanderson,, W. M. Brutinel,, F. R. Cockerill III,, and W. W. Douglas. 1989. Role of bronchoalveolar lavage in the assessment of opportunistic pulmonary infection: utility and complication. Mayo Clin. Proc. 62: 549 557.
6. McQuay, R. M. 1956. Charcoal medium for growth and maintenance of large and small races of Entamoeba histolytica. Am. J. Clin. Pathol. 26: 1137 1138.
1. Arrowood, M. J.,, and C. R. Sterling. 1989. Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection. J. Clin. Microbiol. 27: 1490 1495.
2. Cali, A.,, and R. L. Owen. 1990. Intracellular development of Enterocytozoon, a unique microsporidian found in the intestine of AIDS patients. J. Protozool. 37( 2): 145 155.
3. Canning, E. U.,, and W. S. Hollister. 1990. Enterocytozoon bieneusi (Microspora): prevalence and pathogenicity in AIDS patients. Trans. R. Soc. Trop. Med. Hyg. 84: 181 186.
4.CentersforDiseaseControl. 1990. Microsporidian keratoconjunctivitis in patients with AIDS. MMWR Morb. Mortal. Wkly. Rep. 39: 188189.
5. Derouin, F.,, C. I. Sarfati,, B. Beauvais,, M. Iliou,, L. Dehen,, and M. Lariviere. 1989. Laboratory diagnosis of pulmonary toxoplasmosis in patients with acquired immunodeficiency syndrome. J. Clin. Microbiol. 27: 1661 1663.
6. Fayer, R.,, and B. L. P. Ungar. 1986. Cryptosporidium spp. and cryptosporidiosis . Microbiol. Rev. 50: 458 483.
7. Griffin, J. L., 1978. Pathogenic free-living amoebae, p. 507 549. In J. P. Kreier (ed.), Parasitic Protozoa, vol. 15. Academic Press, Inc., New York, NY.
8. Haley, L. D.,, and P. G. Standard. 1973. Laboratory Methods in Medical Mycology, p. 100. Center for Disease Control, Atlanta, GA.
9. Jones, F. E.,, D. E. Eyles,, N. Coleman,, and C. L. Gibson. 1958. A comparison of methods for the isolation of Toxoplasma from suspected hosts. Am. J. Trop. Med. 7: 531 535.
10. McQuay, R. M. 1956. Charcoal medium for growth and maintenance of large and small races of Entamoeba histolytica. Am. J. Clin. Pathol. 26: 1137 1138.
11. Novy, F. G.,, and W. J. MacNeal. 1904. The cultivation of Trypanosomabrucei. J. Infect. Dis. 1: 1 30.
12. Rijpstra, A. C.,, E. U. Canning,, R. J. Van Ketel,, J. K. M. Eeftinck Schattenkerk,, and J. J. Laarman. 1988. Use of light microscopy to diagnose small-intestinal microsporidiosis in patients with AIDS. J. Infect. Dis. 157: 827 831.
13. Shadduck, J. A. 1989. Humanmicrospori-diosis and AIDS. Rev. Infect. Dis. 11: 203 207.
14. Sterling, C. R.,, and M. J. Arrowood. 1986. Detection of Cryptosporidium sp. Infections using a direct immunofluorescent assay. Pediatr.Infect.Dis. 5: S138 S142.
15. Wheatley, W. B. 1951. A rapid staining procedure for intestinal amoebae and flagellates. Am. J. Clin. Pathol. 21: 990 991.
25. Bryan, R. T., 1990. Microsporidia, p. 2130 2134. In G. L. Mandell,, R. G. Douglas,, and J. E. Bennett (ed.), Principles and Practice of Infectious Diseases, 3rd ed. Churchill Livingstone, NewYork, NY.
26. Bryan, R. T.,, A. Cali,, R. L. Owen,, and H. C. Spencer,. 1991. Microsporidia: opportunistic pathogens in patients with AIDS, p. 1 26. In T. Sun (ed.), Progressin Clinical Parasitology, vol. 2. Field & Wood, Philadelphia, PA.
27. Garcia, L. S. 2007. Diagnostic Medical Parasitology, 5th ed. ASM Press, Washington, DC.
28. Ignatius, R.,, S. Henschel,, O. Liesenfeld,, U. Mansmann,, W. Schmidt,, S. Koppe,, T. Schneider,, W. Heise,, U. Futh,, E. O. Riecken,, H. Hahn,, and R. Ulrich. 1997. Comparative evaluation of modified trichrome and Uvitex 2B stains for detection of low numbers of microsporidial spores in stool specimens. J. Clin. Microbiol. 35: 2266 2269.
29. Jelinek, T.,, S. Eichenlaub,, and T. Loscher. 1999. Sensitivity and specificity of a rapid immunochromatographic test for diagnosis of visceral leishmaniasis. Eur. J. Clin. Microbiol. Infect. Dis. 18: 669 670.
30. Wittner, M.,, and L. M. Weiss. 1999. The Microsporidia and Microsporidiosis. ASM Press, Washington, DC.

Tables

Generic image for table

Phosphate buffer solutions

Citation: Garcia L. 2010. Sputum, Aspirates, and Biopsy Material, p 680-695. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.7
Generic image for table
Table 9.7.3–1

Stains for identifying parasites in various tissues

: In disseminated infections with microsporidia, spores and developing stages from a number of different species could be found throughout the body. Routine histology processing and staining (H&E, silver, Gram, PAS) are used, as are Giemsa, modified trichrome stains, and optical brightening agents. However, the modified trichrome stains (chromotrope) are more commonly used and recommended for clinical specimens other than tissues, such as stool, urine, eye specimens, etc.

EM, electron microscopy.

Citation: Garcia L. 2010. Sputum, Aspirates, and Biopsy Material, p 680-695. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.7

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