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Chapter 9.9 : Culture

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Abstract:

, the agent of intestinal and hepatic amebiasis, can be cultivated in conjunction with the bacteria voided in feces by the infected patient. Although cultures for are not routinely offered by most clinical laboratories, this approach may be helpful when routine procedures have failed to provide a diagnosis. Polyxenic cultured organisms can also be used to produce intestinal and hepatic amebiasis in susceptible experimental hosts such as hamsters, guinea pigs, and rats. Axenic cultivation of organisms is invaluable for the following: (i) to study the biochemistry, physiology, and metabolism of the organisms in order to establish nutritional requirements of the parasites; (ii) to produce antigens of and monoclonal and polyclonal antibodies against for serological diagnosis as well as other immunologic studies; (iii) to differentiate pathogenic from nonpathogenic strains by using isoenzyme electrophoresis, monoclonal antibody, and/or DNA probes; (iv) to screen drugs in vitro to identify isolates susceptible and resistant to particular drugs so that advances in chemotherapy can be evaluated; (v) to infect experimental animals to produce the disease so that pathological processes can be understood; and (vi) to understand the organization of the parasite at the ultrastructural level.

Citation: Garcia L. 2010. Culture, p 739-771. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.9
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Figures

Image of Figure 9.9.4-1
Figure 9.9.4-1

InPouch TV diagnostic system for culturing (BioMed Diagnostics). The swab containing a specimen from the patient is inserted into the liquid medium within the plastic pouch.

Citation: Garcia L. 2010. Culture, p 739-771. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.9
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Image of Figure 9.9.4-2
Figure 9.9.4-2

Illustration of the InPouch TV culture system for (BioMed Diagnostics). From top to bottom: (1) introduction of the specimen into the upper chamber containing a small amount of medium; (2) application of a plastic holder for microscope viewing prior to expressing medium into the lower chamber (optional); (3) transfer of a small amount of medium in the upper chamber to the lower chamber; (4) rolling down the upper chamber and sealing it with tape; (5) plastic viewing frame used to immobilize the medium in the pouch for examination under the microscope. (Diagram courtesy of BioMed Diagnostics; reprinted from , , 5th ed., 2007, ASM Press, Washington, DC.)

Citation: Garcia L. 2010. Culture, p 739-771. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch9.9
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Download as Powerpoint

References

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