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Chapter 8 : Biofilm and

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Biofilm and , Page 1 of 2

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Abstract:

is the most important airborne fungal pathogen in the world. The conidia are inhaled by the entire population and cause a wide range of diseases from simple rhinitis to fatal invasive aspergillosis (IA) in immunocompromised patients ( ). The number of chronic infections is constantly increasing in immunocompetent patients suffering from respiratory problems such as chronic obstructive pulmonary disease (22%), asthma (1 to 5%), and cystic fibrosis (5 to 10%), along with 15% allergic bronchopulmonary aspergillosis. is also the cause of lung and sinus aspergilloma and serious fungal keratitis infections ( ).

Citation: Beauvais A, Latgé J. 2015. Biofilm and , p 149-161. In Ghannoum M, Parsek M, Whiteley M, Mukherjee P (ed), Microbial Biofilms, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MB-0017-2015
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Figures

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Figure 1

Ultrastructure of a human aspergilloma (A) and IA in mouse lung (B) showing the network of hyphae embedded in an ECM. doi:10.1128/microbiolspec.MB-0017-2015.f1

Citation: Beauvais A, Latgé J. 2015. Biofilm and , p 149-161. In Ghannoum M, Parsek M, Whiteley M, Mukherjee P (ed), Microbial Biofilms, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MB-0017-2015
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Figure 2

Immunolabeling of α1,3 glucan on an ultrathin section of an aspergilloma (A) and invasive aspergillosis in mouse lung (B) with a polyclonal rabbit antibody against anti-α1,3 glucan (1/50 diluted; kind gift of Dr. Ohno, Tokyo University of Pharmacy and Life Science, Japan) and an anti-IgG (whole molecule) conjugated with gold 10 nm. doi:10.1128/microbiolspec.MB-0017-2015.f2

Citation: Beauvais A, Latgé J. 2015. Biofilm and , p 149-161. In Ghannoum M, Parsek M, Whiteley M, Mukherjee P (ed), Microbial Biofilms, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MB-0017-2015
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Figure 3

Schematic representation of model 1 and 2 biofilms. Model 1 is in liquid medium, and model 2 is in solid agar medium. Both models are static. Maturation is achieved in 72 h in model 1 and 16 h in model 2 at 37°C. doi:10.1128/microbiolspec.MB-0017-2015.f3

Citation: Beauvais A, Latgé J. 2015. Biofilm and , p 149-161. In Ghannoum M, Parsek M, Whiteley M, Mukherjee P (ed), Microbial Biofilms, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MB-0017-2015
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Figure 4

Biomass of hydrophobin mutants in model 2 biofilm conditions showing the lower biomass of ΔrodD and ΔrodF mutants. *, P < 0.05. doi:10.1128/microbiolspec.MB-0017-2015.f4

Citation: Beauvais A, Latgé J. 2015. Biofilm and , p 149-161. In Ghannoum M, Parsek M, Whiteley M, Mukherjee P (ed), Microbial Biofilms, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MB-0017-2015
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Tables

Generic image for table
TABLE 1

Composition of the extracellular matrix and in model 2 biofilm conditions

Citation: Beauvais A, Latgé J. 2015. Biofilm and , p 149-161. In Ghannoum M, Parsek M, Whiteley M, Mukherjee P (ed), Microbial Biofilms, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MB-0017-2015
Generic image for table
TABLE 2

DHN melanin ( to rows), pyomelanin ( to rows) and hydrophobin ( to rows) gene expression profiles obtained by RNAseq analysis in various conditions

Citation: Beauvais A, Latgé J. 2015. Biofilm and , p 149-161. In Ghannoum M, Parsek M, Whiteley M, Mukherjee P (ed), Microbial Biofilms, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MB-0017-2015

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