1887

Chapter 14 : Recombinant Paper Plasmids

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $30.00

Preview this chapter:
Zoom in
Zoomout

Recombinant Paper Plasmids, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555817480/9781555814724_Chap14-1.gif /docserver/preview/fulltext/10.1128/9781555817480/9781555814724_Chap14-2.gif

Abstract:

Some of the most important techniques used in biotechnology today involve making recombinant DNA molecules. Recombinant DNA molecules are pieces of DNA that have been reassembled from pieces taken from more than one source of DNA. Plasmids are copied by the cell’s DNA replication enzymes because they contain a special sequence of DNA bases called an origin of replication. Plasmids often contain genes for resistance to antibiotics. Plasmids carrying genes for ampicillin and kanamycin resistance are assembled and the two plasmids are recombined. The plasmid with ampicillin resistance is called as pAMP, the plasmid with kanamycin resistance as pKAN, and the recombinant plasmid as pAMP/KAN. Scientists place real recombinant plasmids back into bacteria, where they are replicated. The multiplying bacteria, carrying the recombinant plasmid, generate millions of copies of the recombinant DNA molecule and the proteins it encodes. New copies of the plasmid are synthesized by the cell’s DNA replication enzymes and passed to daughter cells as the bacteria multiply.

Citation: Kreuzer H, Massey A. 2008. Recombinant Paper Plasmids, p 199-201. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch14

Key Concept Ranking

DNA
0.57106405
Bacterial Chromosome
0.48535383
Chromosomes
0.4642198
DNA Ligase
0.44630873
0.57106405
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

References

/content/book/10.1128/9781555817480.chap14

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error