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Chapter 15 : Restriction Analysis Challenge

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Restriction Analysis Challenge, Page 1 of 2

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Abstract:

Restriction analysis is especially important in checking the structures of recombinant DNA molecules and in analyzing an unknown DNA. This chapter shows some examples with some typical restriction analysis problems encountered by scientists in the laboratory. Observing the sizes of fragments produced, scientists determine restriction site locations that would give them the patterns they see. A linear (not circular) piece of DNA is digested with the restriction endonuclease EcoRI and gives fragments of the following sizes: 3,000, 3,600, and 3,400 bp. When digested with BamHI, the DNA molecule gives fragments of the following sizes: 4,500, 3,000, and 2,500 bp. A double digest with EcoRI and BamHI gives fragments of the following sizes: 2,500, 500, 3,600, 3,000, and 400 bp. The distances between the restriction sites in base pairs are indicated, and the sites EcoRI or BamHI are appropriately labeled.

Citation: Kreuzer H, Massey A. 2008. Restriction Analysis Challenge, p 202-204. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch15

Key Concept Ranking

DNA
0.97128266
Vectors
0.54209185
Gene
0.5196939
Endonuclease
0.5160515
Electrophoresis
0.45273107
0.97128266
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Figure 15.1

Information and gel outline for restriction analysis challenge I.

Citation: Kreuzer H, Massey A. 2008. Restriction Analysis Challenge, p 202-204. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch15
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Image of Figure 15.2
Figure 15.2

Information for restriction analysis challenge II.

Citation: Kreuzer H, Massey A. 2008. Restriction Analysis Challenge, p 202-204. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch15
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Image of Figure 15.3
Figure 15.3

Gel outline for restriction analysis challenge IIC.

Citation: Kreuzer H, Massey A. 2008. Restriction Analysis Challenge, p 202-204. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch15
Permissions and Reprints Request Permissions
Download as Powerpoint

References

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