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Chapter 16 : Detection of Specific DNA Sequences: Part I. Fishing for DNA

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Detection of Specific DNA Sequences: Part I. Fishing for DNA, Page 1 of 2

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Abstract:

Scientists often need to fish for particular pieces of DNA, such as the cystic fibrosis gene. When a scientist fishes for a particular piece of DNA, a special DNA “hook” called a probe is used. One type of analysis done with fluorescent probes is called fluorescent (or fluorescence) in situ hybridization (FISH). A FISH analysis consists of applying fluorescently labeled probes to an intact sample, such as a chromosome, a cell, or a thin slice of tissue, rather than to DNA that has been extracted from the sample. The hybridization analysis learnt in can indicate whether a given DNA sequence is present in a sample. In 1975, a scientist named Southern figured out a way to transfer DNA fragments from a gel directly to a membrane, so that the exact pattern of the fragments in the gel was preserved. After the fragments were stuck on the membrane, they could be tested for hybridization to a probe.

Citation: Kreuzer H, Massey A. 2008. Detection of Specific DNA Sequences: Part I. Fishing for DNA, p 205-209. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch16

Key Concept Ranking

Agarose Gel Electrophoresis
0.47780585
DNA Restriction Enzymes
0.4734201
0.47780585
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Figures

Image of Diagram 16.I
Diagram 16.I

Hybridization of a probe to a sequence within single-stranded sample DNA.

Citation: Kreuzer H, Massey A. 2008. Detection of Specific DNA Sequences: Part I. Fishing for DNA, p 205-209. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch16
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Image of Diagram16.II
Diagram16.II

Southern hybridization analysis shows which restriction fragments hybridize to a probe.

Citation: Kreuzer H, Massey A. 2008. Detection of Specific DNA Sequences: Part I. Fishing for DNA, p 205-209. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch16
Permissions and Reprints Request Permissions
Download as Powerpoint

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