1887

Chapter 22 : Nature's Plant Genetic Engineer

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $30.00

Preview this chapter:
Zoom in
Zoomout

Nature's Plant Genetic Engineer, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555817480/9781555814724_Chap22-1.gif /docserver/preview/fulltext/10.1128/9781555817480/9781555814724_Chap22-2.gif

Abstract:

infects certain types of plants (most dicots [plants with two seed leaves], but not mono-cots) at wound sites. Once in the wound, the bacterium injects a segment of its plasmid, called tumor inducing (Ti), into the adjacent living plant cells. This piece of DNA, called transferred DNA (T-DNA), is only one region of the plasmid. The tumor cells synthesize new chemicals that provide nourishment that is critical to the bacterium but useless to the plant. In the late 1970s, plant scientists realized they might be able to take advantage of ' natural genetic engineering abilities. They developed an important method for plant genetic engineering based on this organism and its Ti plasmid. In this method, the T-DNA genes that induce tumor formation and nourish the bacterium are removed from the Ti plasmid and replaced with any gene of interest. The new plasmid is returned to , which is grown in culture so that many bacteria carrying the engineered plasmid are produced. The plants to be engineered are then infected with the bacterium carrying the "designer" Ti plasmid. injects the engineered T-DNA into the plant. Instead of receiving genes for tumor formation, the plant gets the genes inserted into the Ti plasmid by the scientist.

Citation: Kreuzer H, Massey A. 2008. Nature's Plant Genetic Engineer, p 238-240. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch22

Key Concept Ranking

Agrobacterium tumefaciens
0.5147887
Test Tubes
0.46714112
DNA Ligase
0.46342778
0.5147887
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

References

/content/book/10.1128/9781555817480.chap22

Tables

Generic image for table
Untitled

Plant Observation Log

Citation: Kreuzer H, Massey A. 2008. Nature's Plant Genetic Engineer, p 238-240. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch22

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error