1887

Chapter 29 : Mapping a Disease Gene

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $30.00

Preview this chapter:
Zoom in
Zoomout

Mapping a Disease Gene, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555817480/9781555814724_Chap29-1.gif /docserver/preview/fulltext/10.1128/9781555817480/9781555814724_Chap29-2.gif

Abstract:

This chapter explores how DNA markers can be used to map the locations of genes. Maps showing the positions of genes or other inheritable chromosome features, such as short tandem repeats (STRs), as deduced from genetic experiments, are called genetic maps. Genetic mapping is still used, but now it can be combined with physical analyses of DNA to yield much more detailed and accurate information about genes. Genetic mapping was invented long before researchers had the ability to analyze DNA (and before they knew that genes were made of DNA). It is based on the observation of inheritance patterns. Genetic mapping hinges on the measurement of recombination frequency. One recombination event between the nail-patella syndrome (NPS) and ABO loci in 10 paternal haplotypes was observed. The chapter summarizes the frequencies with which alleles of each pair of loci are inherited together. While some scientists were developing better sequencing methods, others focused on the goal of developing detailed maps. To achieve this goal, they needed better mapping techniques and better methods for cloning large amounts of DNA that could be assembled into overlapping fragments. A new discipline within molecular biology, proteomics, focuses on the structures and interactions of proteins.

Citation: Kreuzer H, Massey A. 2008. Mapping a Disease Gene, p 290-302. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch29

Key Concept Ranking

Restriction Fragment Length Polymorphism
0.41259578
0.41259578
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

Figures

Image of Figure 29.1
Figure 29.1

Linked and unlinked inheritance of alleles. +, normal allele of gene; −, mutant, disease-causing allele of gene; 8, STR allele with 8 repeats; 15, STR allele with 15 repeats.

Citation: Kreuzer H, Massey A. 2008. Mapping a Disease Gene, p 290-302. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch29
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Figure 29.2
Figure 29.2

Inheritance of NPS and the ABO blood group.

Citation: Kreuzer H, Massey A. 2008. Mapping a Disease Gene, p 290-302. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch29
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Figure 29.3
Figure 29.3

Genetic mapping based on recombination frequency.

Citation: Kreuzer H, Massey A. 2008. Mapping a Disease Gene, p 290-302. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch29
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Figure 29.4
Figure 29.4

Pedigree of a family afflicted with BFNC.

Citation: Kreuzer H, Massey A. 2008. Mapping a Disease Gene, p 290-302. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch29
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of

Pedigree A and Gel Diagram A (generations I and II). Shaded symbols mean afflicted with BNCS. M, molecular size marker.

Citation: Kreuzer H, Massey A. 2008. Mapping a Disease Gene, p 290-302. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch29
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of

Pedigree B and Gel Diagram B (generation III). M, molecular size marker.

Citation: Kreuzer H, Massey A. 2008. Mapping a Disease Gene, p 290-302. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch29
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of

Pedigree C and Gel Diagram C (generation IV). M, molecular size marker.

Citation: Kreuzer H, Massey A. 2008. Mapping a Disease Gene, p 290-302. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch29
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Untitled
Untitled

Citation: Kreuzer H, Massey A. 2008. Mapping a Disease Gene, p 290-302. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch29
Permissions and Reprints Request Permissions
Download as Powerpoint

References

/content/book/10.1128/9781555817480.chap29
1. Collins, F.,, M. Morgan,, and A. Patrinos. 2003. The Human Genome Project: lessons from large-scale biology. Science 300:286290.
2. Frazier, M.,, G. Johnson,, D. Thomassen,, C. Oliver,, and A. Patrinos. 2003. Realizing the potential of the genome revolution: the Genomes to Life Program. Science 300: 290293.

Tables

Generic image for table
Table 29.1

Paternal haplotypes inherited by members of Generation II in Figure 29.2

Citation: Kreuzer H, Massey A. 2008. Mapping a Disease Gene, p 290-302. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch29
Generic image for table
Table 29.2

Frequency of allele combinations in generation IIa

Citation: Kreuzer H, Massey A. 2008. Mapping a Disease Gene, p 290-302. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch29

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error