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Chapter 23 : Metabolism of Aromatic Compounds

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Metabolism of Aromatic Compounds, Page 1 of 2

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Abstract:

There has been a long-standing fundamental and practical interest in microbial metabolism of aromatic compounds. The goal of this chapter is to provide an overview of the generally used, well-established methods that are the hallmark of aromatic metabolism studies. Growing cells in large liquid volumes such as in a fermentor or chemostat is a challenge as well. The high aeration rate of these cultures strips volatile aromatic substrates out of the medium. Toluene and related aromatic compounds have long been used as models for the analysis of aromatic hydrocarbon degradation due to the fact that there are several catabolic pathways known for its degradation, illustrating the many possible ways by which aromatic hydrocarbons can be metabolized. Bacterial metabolism of aromatic hydrocarbons can result in a variety of chemically different compounds, resulting from the ring oxidation and the later ring cleavage steps. These metabolites can be either neutral or charged and either chemically stable or unstable, and thus, care must be taken in the extraction process to stabilize and competently extract the chemicals. If one can identify a -dihydrodiol intermediate in culture supernatants, then a priori the pathway proceeds via an initial dioxygenase attack of the aromatic nucleus. However, if one detects only phenolic or dihydroxylated products in the culture medium then it is uncertain whether the catabolic pathway proceeds by an initial dioxygenase or by two initial monooxygenases.

Citation: Kukor J, Wawrik B, Zylstra G. 2007. Metabolism of Aromatic Compounds, p 586-595. In Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L (ed), Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817497.ch23

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Aromatic Hydrocarbon Degradation
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Chemicals
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High-Performance Liquid Chromatography
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FIGURE 1

Method for growing bacteria in the presence of volatile liquid aromatic hydrocarbons either in a shake flask (A) or on a solid medium (B).

Citation: Kukor J, Wawrik B, Zylstra G. 2007. Metabolism of Aromatic Compounds, p 586-595. In Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L (ed), Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817497.ch23
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Image of FIGURE 2
FIGURE 2

Photograph of bacterial colonies on a plate that has phenanthrene applied to it by the spray plate technique. As the colonies grow, they form clear zones, indicating that the substrate is being utilized. This photograph was kindly provided by J. Philp, Napier University, Edinburgh, United Kingdom.

Citation: Kukor J, Wawrik B, Zylstra G. 2007. Metabolism of Aromatic Compounds, p 586-595. In Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L (ed), Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817497.ch23
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Image of FIGURE 3
FIGURE 3

Representative known pathways for the degradation of toluene illustrating typical reactions catalyzed by enzymes that act on aromatic compounds.

Citation: Kukor J, Wawrik B, Zylstra G. 2007. Metabolism of Aromatic Compounds, p 586-595. In Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L (ed), Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817497.ch23
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