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Chapter 32 : Genetic Exchange in Gram-Positive Bacteria

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Abstract:

This chapter presents an overview of gene transfer systems and their applications in several important groups of gram-positive bacteria. Bacteriophage-mediated generalized transduction is an important method of genetic manipulation that can be accomplished using either lytic or temperate phages. The competent state is transiently induced when a culture of achieves a critical cell density. Whether or not this element retains the ability to transfer itself to other or has become inactivated by mutation remains to be determined. A section focuses on the lactococci and related food-fermenting bacteria. In the case of organisms like , the relative ease of transformation- and conjugation-based transfer methods might account for this trend. The use of the gene and the * cassette in the development of a counter selection system for described elsewhere in the chapter may be adaptable to various LAB strains, although this will need to be tested for each species. The primary focus of the chapter is on advances that have been developed for genetic analysis of and also on some basic protocols for particularly important types of genetic manipulation. In contrast to the gram bacteria, members of the genus belong to the cluster of so-called high-GC gram bacteria based on the nucleotide composition of their genomes.

Citation: Kristich C, Salomon C, Dunny G. 2007. Genetic Exchange in Gram-Positive Bacteria, p 756-799. In Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L (ed), Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817497.ch32

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Mobile Genetic Elements
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Genetic Recombination
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Genetic Elements
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Bacterial Proteins
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Microbial Evolution
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Type IV Secretion Systems
0.4166153
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FIGURE 1

Unrooted phylogenetic tree of selected gram bacteria based on 16S rRNA sequences. The sequences for each organism were obtained from the NCBI database and subjected to phylogenetic analysis assuming maximum parsimony and bootstrapping (500 times resampling). The length of the lines between the species corresponds to phylogenetic distance.

Citation: Kristich C, Salomon C, Dunny G. 2007. Genetic Exchange in Gram-Positive Bacteria, p 756-799. In Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L (ed), Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817497.ch32
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1. Adams, V.,, D. Lyras,, K. A. Farrow,, and J. I. Rood. 2002. The clostridial mobilisable transposons. Cell. Mol. Life Sci. 59: 2033 2043.
2. Akerley, B. J.,, E. J. Rubin,, A. Camilli,, D. J. Lampe,, H. M. Robertson,, and J. J. Mekalanos. 1998. Systematic identification of essential genes by in vitro mariner mutagenesis. Proc. Natl. Acad. Sci. USA 95: 8927 8932.
3. Allison, G. E.,, and T. R. Klaenhammer. 1996. Functional analysis of the gene encoding immunity to lactacin F, lafI, and its use as a Lactobacillus-specific, food-grade genetic marker. Appl. Environ. Microbiol. 62: 4450 4460.
4. Anderson, T.,, P. Brian,, P. Riggle,, R. Kong,, and W. Champness. 1999. Genetic suppression analysis of nonantibiotic-producing mutants of the Streptomyces coelicolor absA locus. Microbiology 145: 2343 2353.
5. Arbeit, R. D., 1997. Laboratory procedures for epidemiologic analysis, p. 253 286. In K. B. Crossley, and G. L. Archer (ed.), The Staphylococci in Human Disease. Churchill Livingstone, New York, NY.
6. Arbeloa, A.,, H. Segal,, J.-E. Hugonnet,, N. Josseaume,, L. Dubost,, J.-P. Brouard,, L. Gutmann,, D. Mengin-Lecreulx,, and M. Arthur. 2004. Role of class A penicillin-binding proteins in PBP5-mediated β-lactam resistance in Enterococcus faecalis. J. Bacteriol. 186: 1221 1228.
7. Avery, O. T.,, C. M. Macleod,, and M. McCarty. 1944. Studies on the chemical nature of the substance inducing transformation of pneumococcal phenotypes. Induction of transformation by a deoxyribonucleic acid fraction isolated from Pneumococcus Type III. J. Exp. Med. 79: 137 158.
8. Awad, M. M.,, A. E. Bryant,, D. L. Stevens,, and J. I. Rood. 1995. Virulence studies on chromosomal alpha-toxin and theta-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of alpha-toxin in Clostridium perfringens-mediated gas gangrene. Mol. Microbiol. 15: 191 202.
9. Ayoubi, P.,, A. O. Kilic,, and M. N. Vijayakumar. 1991. Tn 5253, the pneumococcal omega ( cat tet) BM6001 element, is a composite structure of two conjugative transposons, Tn 5251 and Tn 5252. J. Bacteriol. 173: 1617 1622.
10. Bachrach, G.,, M. Leizerovici-Zigmond,, A. Zlotkin,, R. Naor,, and D. Steinberg. 2003. Bacteriophage isolation from human saliva. Lett. Appl. Microbiol. 36: 50 53.
11. Bae, T.,, A. K. Banger,, A. Wallace,, E. M. Glass,, F. Aslund,, O. Schneewind,, and D. M. Missiakas. 2004. Staphylococcus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing. Proc. Natl. Acad. Sci. USA 101: 12312 12317.
12. Bae, T.,, S. Clerc-Bardin,, and G. M. Dunny. 2000. Analysis of expression of prgX, a key negative regulator of the transfer of the Enterococcus faecalis pheromone-inducible plasmid pCF10. J. Mol. Biol. 297: 861 875.
13. Bae, T.,, B. Kozlowicz,, and G. M. Dunny. 2002. Two targets in pCF10 DNA for PrgX binding: their role in production of Qa and prgX mRNA and in regulation of pheromone-inducible conjugation. J. Mol. Biol. 315: 995 1007.
14. Bailey, C. R.,, C. J. Bruton,, M. J. Butler,, K. F. Chater,, J. E. Harris,, and D. A. Hopwood. 1986. Properties of in vitro recombinant derivatives of pJV1, a multi-copy plasmid from Streptomyces phaeochromogenes. J. Gen. Microbiol. 132: 2071 2078.
15. Bartilson, M.,, A. Marra,, J. Christine,, J. S. Asundi,, W. P. Schneider,, and A. E. Hromockyj. 2001. Differential fluorescence induction reveals Streptococcus pneumoniae loci regulated by competence stimulatory peptide. Mol. Microbiol. 39: 126 135.
15a.. Battisti, L.,, B. D. Green,, and C. B. Thorne. 1985. Mating system for transfer of plasmids among Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis. J. Bacteriol. 162: 543 550.
16. Beard, S. J.,, V. Salisbury,, R. J. Lewis,, J. A. Sharpe,, and A. P. MacGowan. 2002. Expression of lux genes in a clinical isolate of Streptococcus pneumoniae: using bioluminescence to monitor gemifloxacin activity. Antimicrob. Agents Chemother. 46: 538 542.
17. Beck, E.,, G. Ludwig,, E. A. Auerswald,, B. Reiss,, and H. Schaller. 1982. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 19: 327 336.
18. Beltramo, C.,, M. Oraby,, G. Bourel,, D. Garmyn,, and J. Guzzo. 2004. A new vector, pGID052, for genetic transfer in Oenococcus oeni. FEMS Microbiol. Lett. 236: 53 60.
19. Berg, T.,, N. Firth,, S. Apisiridej,, A. Hettiaratchi,, A. Leelaporn,, and R. A. Skurray. 1998. Complete nucleotide sequence of pSK41: evolution of staphylococcal conjugative multiresistance plasmids. J. Bacteriol. 180: 4350 4359.
20. Bertram, J.,, M. Stratz,, and P. Durre. 1991. Natural transfer of conjugative transposon Tn 916 between grampositive and gram-negative bacteria. J. Bacteriol. 173: 443 448.
21. Betley, M. J.,, and J. J. Mekalanos. 1985. Staphylococcal enterotoxin A is encoded by phage. Science 229: 185 187.
22. Betschel, S. D.,, S. M. Borgia,, N. L. Barg,, D. E. Low,, and J. C. De Azavedo. 1998. Reduced virulence of group A streptococcal Tn 916 mutants that do not produce streptolysin S. Infect. Immun. 66: 1671 1679.
23. Bhatt, A.,, H. M. Kieser,, R. E. Melton,, and T. Kieser. 2002. Plasmid transfer from Streptomyces to Mycobacterium smegmatis by spontaneous transformation. Mol. Microbiol. 43: 135 146.
24. Bhavsar, A. P.,, X. Zhao,, and E. D. Brown. 2001. Development and characterization of a xylose-dependent system for expression of cloned genes in Bacillus subtilis: conditional complementation of a teichoic acid mutant. Appl. Environ. Microbiol. 67: 403 410.
25. Bibb, M. J.,, and S. N. Cohen. 1982. Gene expression in Streptomyces: construction and application of promoterprobe plasmid vectors in Streptomyces lividans. Mol. Gen. Genet. 187: 265 277.
26. Bibb, M. J.,, J. M. Ward,, and S. N. Cohen. 1985. Nucleotide sequences encoding and promoting expression of 3 antibiotic resistance genes indigenous to Streptomyces. Mol. Gen. Genet. 199: 26 36.
27. Bibb, M. J.,, J. M. Ward,, and D. A. Hopwood. 1978. Transformation of plasmid DNA into Streptomyces at high frequency. Nature 274: 398 400.
28. Bierman, M.,, R. Logan,, K. Obrien,, E. T. Seno,, R. N. Rao,, and B. E. Schoner. 1992. Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116: 43 49.
29. Bishop, A.,, S. Fielding,, P. Dyson,, and P. Herron. 2004. Systematic insertional mutagenesis of a streptomycete genome: a link between osmoadaptation and antibiotic production. Genome Res. 14: 893 900.
30. Biswas, I.,, A. Gruss,, S. D. Ehrlich,, and E. Maguin. 1993. High-efficiency gene inactivation and replacement system for gram-positive bacteria. J. Bacteriol. 175: 3628 3635.
31. Biswas, I.,, and J. R. Scott. 2003. Identification of rocA, a positive regulator of covR expression in the group A streptococcus. J. Bacteriol. 185: 3081 3090.
32. Brau, B.,, U. Pilz,, and W. Piepersberg. 1984. Genes for gentamicin-(3)-N-acetyltransferases-III and gentamicin-(3)-N-acetyltransferase-IV. I. Nucleotide sequence of the aac(3)-IV gene and possible involvement of an IS 140 element in its expression. Mol. Gen. Genet. 193: 179 187.
33. Braunstein, M.,, S. S. Bardarov,, and W. R. Jacobs, Jr. 2002. Genetic methods for deciphering virulence determinants of Mycobacterium tuberculosis. Methods Enzymol. 358: 67 99.
34. Bricker, A. L.,, and A. Camilli. 1999. Transformation of a type 4 encapsulated strain of Streptococcus pneumoniae. FEMS Microbiol. Lett. 172: 131 135.
35. Bringel, F.,, G. L. Van Alstine,, and J. R. Scott. 1991. A host factor absent from Lactococcus lactis subspecies lactis MG1363 is required for conjugative transposition. Mol. Microbiol. 5: 2983 2993.
36. Bringel, F.,, G. L. Van Alstine,, and J. R. Scott. 1992. Transfer of Tn 916 between Lactococcus lactis subsp. lactis strains is nontranspositional: evidence for a chromosomal fertility function in strain MG1363. J. Bacteriol. 174: 5840 5847.
37. Bron, P. A.,, M. G. Benchimol,, J. Lambert,, E. Palumbo,, M. Deghorain,, J. Delcour,, W. M. de Vos,, M. Kleerebezem,, and P. Hols. 2002. Use of the alr gene as a foodgrade selection marker in lactic acid bacteria. Appl. Environ. Microbiol. 68: 5663 5670.
38. Bron, P. A.,, S. M. Hoffer,, S. Van II,, W. M. De Vos,, and M. Kleerebezem. 2004. Selection and characterization of conditionally active promoters in Lactobacillus plantarum, using alanine racemase as a promoter probe. Appl. Environ. Microbiol. 70: 310 317.
39. Bron, S., 1990. Plasmids, p. 75 174. In C. R. Harwood, and S. M. Cutting (ed.), Molecular Biological Methods for Bacillus. John Wiley & Sons, Chichester, United Kingdom.
40. Brussow, H. 2001. Phages of dairy bacteria. Annu. Rev. Microbiol. 55: 283 303.
41. Bryan, E. M.,, T. Bae,, M. Kleerebezem,, and G. M. Dunny. 2000. Improved vectors for nisin-controlled expression in gram-positive bacteria. Plasmid 44: 183 190.
42. Buckley, N. D.,, C. Vadeboncoeur,, D. J. LeBlanc,, L. N. Lee,, and M. Frenette. 1999. An effective strategy, applicable to Streptococcus salivarius and related bacteria, to enhance or confer electroporation competence. Appl. Environ. Microbiol. 65: 3800 3804.
43. Burke, J.,, D. Schneider,, and J. Westpheling. 2001. Generalized transduction in Streptomyces coelicolor. Proc. Natl. Acad. Sci. USA 98: 6289 6294.
44. Burrus, V.,, G. Pavlovic,, B. Decaris,, and G. Guedon. 2002. Conjugative transposons: the tip of the iceberg. Mol. Microbiol. 46: 601 610.
45. Buu-Hoi, A.,, G. Bieth,, and T. Horaud. 1984. Broad host range of streptococcal macrolide resistance plasmids. Antimicrob. Agents Chemother. 25: 289 291.
46. Caparon, M., 2000. Genetics of group A streptococci, p. 53 65. In V. A. Fischetti,, R. P. Novick,, J. J. Ferretti,, D. A. Portnoy,, and J. I. Rood (ed.), Gram-Positive Pathogens. ASM Press, Washington, DC.
47. Caparon, M. G.,, R. T. Geist,, J. Perez-Casal,, and J. R. Scott. 1992. Environmental regulation of virulence in group A streptococci: transcription of the gene encoding M protein is stimulated by carbon dioxide. J. Bacteriol. 174: 5693 5701.
48. Caparon, M. G.,, and J. R. Scott. 1987. Identification of a gene that regulates expression of M protein, the major virulence determinant of group A streptococci. Proc. Natl. Acad. Sci. USA 84: 8677 8681.
49. Caparon, M. G.,, and J. R. Scott. 1991. Genetic manipulation of pathogenic streptococci. Methods Enzymol. 204: 556 586.
50. Caprioli, T.,, F. Zaccour,, and S. S. Kasatiya. 1975. Phage typing scheme for group D streptococci isolated from human urogenital tract. J. Clin. Microbiol. 2: 311 317.
51. Cascales, E.,, and P. J. Christie. 2003. The versatile bacterial type IV secretion systems. Nat. Rev. Microbiol. 1: 137 149.
52. Casey, J.,, C. Daly,, and G. F. Fitzgerald. 1991. Chromosomal integration of plasmid DNA by homologous recombination in Enterococcus faecalis and Lactococcus lactis subsp. lactis hosts harboring Tn 919. Appl. Environ. Microbiol. 57: 2677 2682.
53. Chan, P. F.,, K. M. O’Dwyer,, L. M. Palmer,, J. D. Ambrad,, K. A. Ingraham,, C. So,, M. A. Lonetto,, S. Biswas,, M. Rosenberg,, D. J. Holmes,, and M. Zalacain. 2003. Characterization of a novel fucose-regulated promoter (P fcsK) suitable for gene essentiality and antibacterial mode-of-action studies in Streptococcus pneumoniae. J. Bacteriol. 185: 2051 2058.
54. Charpentier, E.,, A. I. Anton,, P. Barry,, B. Alfonso,, Y. Fang,, and R. P. Novick. 2004. Novel cassette-based shuttle vector system for gram-positive bacteria. Appl. Environ. Microbiol. 70: 6076 6085.
55. Chen, I.,, and D. Dubnau. 2003. DNA transport during transformation. Front. Biosci. 8: s544 556.
56. Chen, I.,, and D. Dubnau. 2004. DNA uptake during bacterial transformation. Nat. Rev. Microbiol. 2: 241 249.
57. Chen, L. Y.,, W. M. Leu,, K. T. Wang,, and Y. H. W. Lee. 1992. Copper transfer and activation of the Streptomyces apotyrosinase are mediated through a complex formation between apotyrosinase and its trans-activator MelC1. J. Biol. Chem. 267: 20100 20107.
58. Chen, Y. S.,, and J. L. Steele. 2005. Analysis of promoter sequences from Lactobacillus helveticus CNRZ32 and their activity in other lactic acid bacteria. J. Appl. Microbiol. 98: 64 72.
59. Cheung, A. L.,, J. M. Koomey,, C. A. Butler,, S. J. Projan,, and V. A. Fischetti. 1992. Regulation of exoprotein expression in Staphylococcus aureus by a locus ( sar) distinct from agr. Proc. Natl. Acad. Sci. USA 89: 6462 6466.
60. Cheung, A. L.,, C. Wolz,, M. R. Yeaman,, and A. S. Bayer. 1995. Insertional inactivation of a chromosomal locus that modulates expression of potential virulence determinants in Staphylococcus aureus. J. Bacteriol. 177: 3220 3226.
61. Christie, P. J. 2001. Type IV secretion: intercellular transfer of macromolecules by systems ancestrally related to conjugation machines. Mol. Microbiol. 40: 294 305.
62. Cirillo, J. D.,, R. G. Barletta,, B. R. Bloom,, and W. R. Jacobs, Jr. 1991. A novel transposon trap for mycobacteria: isolation and characterization of IS1096. J. Bacteriol. 173: 7772 7780.
63. Clancy, A.,, M. H. Lee,, A. L. Jones,, and C. E. Rubens. 2004. Construction and characterization of transposon Tn phoZ for the identification of genes encoding exported proteins in Streptococcus agalactiae. FEMS Microbiol. Lett. 241: 257 264.
64. Claverys, J. P.,, A. Dintilhac,, E. V. Pestova,, B. Martin,, and D. A. Morrison. 1995. Construction and evaluation of new drug-resistance cassettes for gene disruption mutagenesis in Streptococcus pneumoniae, using an ami test platform. Gene 164: 123 128.
65. Claverys, J. P.,, and L. S. Havarstein. 2002. Extracellularpeptide control of competence for genetic transformation in Streptococcus pneumoniae. Front. Biosci. 7: 1798 1814.
66. Claverys, J. P.,, and B. Martin. 2003. Bacterial “competence” genes: signatures of active transformation, or only remnants? Trends Microbiol. 11: 161 165.
67. Claverys, J. P.,, M. Prudhomme,, I. Mortier-Barriere,, and B. Martin. 2000. Adaptation to the environment: Streptococcus pneumoniae, a paradigm for recombination-mediated genetic plasticity? Mol. Microbiol. 35: 251 259.
68. Clewell, D. B. 1981. Plasmids, drug resistance, and gene transfer in the genus Streptococcus. Microbiol. Rev. 45: 409 436.
69. Clewell, D. B.,, F. Y. An,, B. A. White,, and C. Gawron-Burke. 1985. Streptococcus faecalis sex pheromone (cAM373) also produced by Staphylococcus aureus and identification of a conjugative transposon (Tn918). J. Bacteriol. 162: 1212 1220.
70. Clewell, D. B.,, and G. M. Dunny,. 2002. Conjugation and genetic exchange in enterococci, p. 265 300. In M. S. Gilmore,, D. B. Clewell,, P. Courvalin,, G. M. Dunny,, B. E. Murray,, and L. B. Rice (ed.), The Enterococci: Pathogenesis, Molecular Biology, and Antibiotic Resistance. ASM Press, Washington, DC.
71. Clewell, D. B.,, S. E. Flannagan,, and D. D. Jaworski. 1995. Unconstrained bacterial promiscuity: the Tn 916-Tn 1545 family of conjugative transposons. Trends Microbiol. 3: 229 236.
72. Clewell, D. B.,, M. V. Francia,, S. E. Flannagan,, and F. Y. An. 2002. Enterococcal plasmid transfer: sex pheromones, transfer origins, relaxases, and the Staphylococcus aureus issue. Plasmid 48: 193 201.
73. Clewell, D. B.,, and C. Gawron-Burke. 1986. Conjugative transposons and the dissemination of antibiotic resistance in streptococci. Annu. Rev. Microbiol. 40: 635 659.
74. Clewell, D. B.,, P. K. Tomich,, M. C. Gawron-Burke,, A. E. Franke,, Y. Yagi,, and F. Y. An. 1982. Mapping of Streptococcus faecalis plasmids pAD1 and pAD2 and studies relating to transposition of Tn 917. J. Bacteriol. 152: 1220 1230.
75. Clewell, D. B.,, Y. Yagi,, G. M. Dunny,, and S. K. Schultz. 1974. Characterization of three plasmid deoxyribonucleic acid molecules in a strain of Streptococcus faecalis: identification of a plasmid determining erythromycin resistance. J. Bacteriol. 117: 283 289.
76. Coleman, D.,, J. Knights,, R. Russell,, D. Shanley,, T. H. Birkbeck,, G. Dougan,, and I. Charles. 1991. Insertional inactivation of the Staphylococcus aureus beta-toxin by bacteriophage phi 13 occurs by site- and orientation-specific integration of the phi 13 genome. Mol. Microbiol. 5: 933 939.
77. Corbisier, P.,, G. Ji,, G. Nuyts,, M. Mergeay,, and S. Silver. 1993. luxAB gene fusions with the arsenic and cadmium resistance operons of Staphylococcus aureus plasmid pI258. FEMS Microbiol. Lett. 110: 231 238.
78. Courvalin, P.,, and C. Carlier. 1986. Transposable multiple antibiotic resistance in Streptococcus pneumoniae. Mol. Gen. Genet. 205: 291 297.
79. Cousineau, B.,, D. Smith,, S. Lawrence-Cavanagh,, J. E. Mueller,, J. Yang,, D. Mills,, D. Manias,, G. Dunny,, A. M. Lambowitz,, and M. Belfort. 1998. Retrohoming of a bacterial group II intron: mobility via complete reverse splicing, independent of homologous DNA recombination. Cell 94: 451 462.
80. Cruz-Rodz, A. L.,, and M. S. Gilmore. 1990. High efficiency introduction of plasmid DNA into glycine treated Enterococcus faecalis by electroporation. Mol. Gen. Genet. 224: 152 154.
81. Cutting, S. M.,, and P. B. Vanderhorn,. 1990. Genetic analysis, p. 27 74. In C. R. Harwood ,and S. M. Cutting (ed.), Molecular Biological Methods for Bacillus. John Wiley & Sons, Chichester, United Kingdom.
82. Cutting, S. M.,, and P. Youngman,. 1994. Gene transfer in gram-positive bacteria, p. 348 364. In P. Gerhardt,, R. G. E. Murray,, W. A. Wood,, and N. R. Krieg (ed.), Methods for General and Molecular Bacteriology. ASM Press, Washington, DC.
83. Cvitkovitch, D. G. 2001. Genetic competence and transformation in oral streptococci. Crit. Rev. Oral Biol. Med. 12: 217 243.
84. Dagkessamanskaia, A.,, M. Moscoso,, V. Henard,, S. Guiral,, K. Overweg,, M. Reuter,, B. Martin,, J. Wells,, and J. P. Claverys. 2004. Interconnection of competence, stress and CiaR regulons in Streptococcus pneumoniae: competence triggers stationary phase autolysis of ciaR mutant cells. Mol. Microbiol. 51: 1071 1086.
85. Datsenko, K. A.,, and B. L. Wanner. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. USA 97: 6640 6645.
86. Davis, N. K.,, and K. F. Chater. 1990. Spore color in Streptomyces coelicolor A3(2) involves the developmentally regulated synthesis of a compound biosynthetically related to polyketide antibiotics. Mol. Microbiol. 4: 1679 1691.
86a.. Day, W. A., Jr.,, S. L. Rasmussen,, B. M. Carpenter,, S. N. Peterson,, and A. M. Friedlander. 2007. Microarray analysis of transposon insertion mutations in Bacillus anthracis: global identification of genes required for sporulation and germination. J. Bacteriol. 189: 3296 3301.
87. De Boever, E. H.,, D. B. Clewell,, and C. M. Fraser. 2000. Enterococcus faecalis conjugative plasmid pAM373: complete nucleotide sequence and genetic analyses of sex pheromone response. Mol. Microbiol. 37: 1327 1341.
88. Dedonder, R. 1966. Levan sucrase from Bacillus subtilis. Methods Enzymol. 8: 500 505.
89. de la Cruz, F.,, and J. Davies. 2000. Horizontal gene transfer and the origin of species: lessons from bacteria. Trends Microbiol. 8: 128 133.
90. De Las Rivas, B.,, J. L. García,, R. López,, and P. García. 2002. Purification and polar localization of pneumococcal LytB, a putative endo-β- N-acetylglucosaminidase: the chain-dispersing murein hydrolase. J. Bacteriol. 184: 4988 5000.
91. Derbyshire, K. M.,, and S. S. Bardarov,. 2000. DNA transfer in mycobacteria: conjugation and transduction, p. 93 110. In G. F. Hatfull, and W. R. Jacobs, Jr. (ed.), Molecular Genetics of Mycobacteria. ASM Press, Washington, DC.
92. de Ruyter, P. G.,, O. P. Kuipers,, and W. M. de Vos. 1996. Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. Appl. Environ. Microbiol. 62: 3662 3667.
93. Desiere, F.,, S. Lucchini,, C. Canchaya,, M. Ventura,, and H. Brussow. 2002. Comparative genomics of phages and prophages in lactic acid bacteria. Antonie Leeuwenhoek 82: 73 91.
94. de Vos, W. M. 1999. Gene expression systems for lactic acid bacteria. Curr. Opin. Microbiol. 2: 289 295.
95. Dieye, Y.,, S. Usai,, F. Clier,, A. Gruss,, and J. C. Piard. 2001. Design of a protein-targeting system for lactic acid bacteria. J. Bacteriol. 183: 4157 4166.
96. Dillard, J. P.,, and J. Yother. 1994. Genetic and molecular characterization of capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 3. Mol. Microbiol. 12: 959 972.
97. Doi, K.,, Y. Ono,, E. Yokoyama,, Y. Tsukagoe,, and S. Ogata. 1998. Whole sequence of spoIIIE-like, sporulation-inhibitory, and transfer gene ( spi) in a conjugative plasmid, pSA1.1, of Streptomyces azureus and detection of spi-like gene in the actinomycete chromosome. Biosci. Biotechnol. Biochem. 62: 1597 1600.
98. Donnelly-Wu, M. K.,, W. R. Jacobs, Jr.,, and G. F. Hatfull. 1993. Superinfection immunity of mycobacteriophage L5: applications for genetic transformation of mycobacteria. Mol. Microbiol. 7: 407 417.
99. Doolittle, W. F. 1999. Phylogenetic classification and the universal tree. Science 284: 2124 2129.
100. Dougherty, B. A.,, and I. van de Rijn. 1992. Molecular characterization of a locus required for hyaluronic acid capsule production in group A streptococci. J. Exp. Med. 175: 1291 1299.
101. Dubnau, D., 1993. Genetic exchange and homologous recombination, p. 555 584. In A. L. Sonenshein,, J. A. Hoch,, and R. Losick (ed.), Bacillus subtilis and Other Gram-Positive Bacteria: Biochemistry, Physiology, and Molecular Genetics. American Society for Microbiology, Washington, DC.
102. Dubnau, D.,, and C. M. Lovett,. 2002. Transformation and recombination, p. 453 471. In A. L. Sonenshein,, J. A. Hoch,, and R. Losick (ed.), Bacillus subtilis and Its Closest Relatives: from Genes to Cells. ASM Press, Washington, DC.
102a.. Dunny, G. M.,, P. P. Cleary,, and L. L. McKay (ed.). 1991. Genetics and Molecular Biology of Streptococci, Lactococci, and Enterococci. American Society for Microbiology, Washington, DC.
103. Dunny, G. M.,, and D. B. Clewell. 1975. Transmissible toxin (hemolysin) plasmid in Streptococcus faecalis and its mobilization of a noninfectious drug resistance plasmid. J. Bacteriol. 124: 784 790.
104. Dunny, G. M.,, L. N. Lee,, and D. J. LeBlanc. 1991. Improved electroporation and cloning vector system for gram-positive bacteria. Appl. Environ. Microbiol. 57: 1194 1201.
105. Duvall, E. J.,, D. M. Williams,, P. S. Lovett,, C. Rudolph,, N. Vasantha,, and M. Guyer. 1983. Chloramphenicolinducible gene expression in Bacillus subtilis. Gene 24: 171 177.
106. Duwat, P.,, K. Hammer,, A. Bolotin,, and A. Gruss,. 2000. Genetics of lactococci, p. 295 306. In V. A. Fischetti,, R. P. Novick,, J. J. Ferretti,, D. A. Portnoy,, and J. I. Rood (ed.), Gram-Positive Pathogens. ASM Press, Washington, DC.
107. Dyer, D. W.,, M. I. Rock,, C. Y. Lee,, and J. J. Iandolo. 1985. Generation of transducing particles in Staphylococcus aureus. J. Bacteriol. 161: 91 95.
108. Eichenbaum, Z.,, M. J. Federle,, D. Marra,, W. M. de Vos,, O. P. Kuipers,, M. Kleerebezem,, and J. R. Scott. 1998. Use of the lactococcal nisA promoter to regulate gene expression in gram-positive bacteria: comparison of induction level and promoter strength. Appl. Environ. Microbiol. 64: 2763 2769.
109. Eichenbaum, Z.,, and J. R. Scott. 1997. Use of Tn 917 to generate insertion mutations in the group A streptococcus. Gene 186: 213 217.
110. Elsner, A.,, B. Kreikemeyer,, A. Braun-Kiewnick,, B. Spellerberg,, B. A. Buttaro,, and A. Podbielski. 2002. Involvement of Lsp, a member of the LraI-lipoprotein family in Streptococcus pyogenes, in eukaryotic cell adhesion and internalization. Infect. Immun. 70: 4859 4869.
111. Engel, H. W.,, N. Soedirman,, J. A. Rost,, W. J. van Leeuwen,, and J. D. van Embden. 1980. Transferability of macrolide, lincomycin, and streptogramin resistances between group A, B, and D streptococci, Streptococcus pneumoniae, and Staphylococcus aureus. J. Bacteriol. 142: 407 413.
112. English, R. S.,, J. S. Lampel,, and T. J. Vanden Boom. 1998. Transformation of Saccharopolyspora erythraea by electroporation of germinating spores: construction of propionyl Co-A carboxylase mutants. J. Ind. Microbiol. Biotechnol. 21: 219 224.
113. Evans, J.,, and K. G. Dyke. 1988. Characterization of the conjugation system associated with the Staphylococcus aureus plasmid pJE1. J. Gen. Microbiol. 134(Pt. 1): 1 8.
114. Fabret, C.,, S. D. Ehrlich,, and P. Noirot. 2002. A new mutation delivery system for genome-scale approaches in Bacillus subtilis. Mol. Microbiol. 46: 25 36.
115. Feitelson, J. S. 1988. An improved plasmid for the isolation and analysis of Streptomyces promoters. Gene 66: 159 162.
116. Feucht, A.,, and P. J. Lewis. 2001. Improved plasmid vectors for the production of multiple fluorescent protein fusions in Bacillus subtilis. Gene 264: 289 297.
117. Firth, N.,, K. P. Ridgway,, M. E. Byrne,, P. D. Fink,, L. Johnson,, I. T. Paulsen,, and R. A. Skurray. 1993. Analysis of a transfer region from the staphylococcal conjugative plasmid pSK41. Gene 136: 13 25.
118. Fisher, S. H.,, C. J. Bruton,, and K. F. Chater. 1987. The glucose kinase gene of Streptomyces coelicolor and its use in selecting spontaneous deletions for desired regions of the genome. Mol. Gen. Genet. 206: 35 44.
119. Fitzgerald, G. F.,, and D. B. Clewell. 1985. A conjugative transposon (Tn 919) in Streptococcus sanguis [ sic]. Infect. Immun. 47: 415 420.
120. Fitzgerald, G. F.,, and M. J. Gasson. 1988. In vivo gene transfer systems and transposons. Biochimie 70: 489 502.
121. Fitzgerald, N. B.,, R. S. English,, J. S. Lampel,, and T. J. Vanden Boom. 1998. Sonication-dependent electroporation of the erythromycin-producing bacterium Saccharopolyspora erythraea. Appl. Environ. Microbiol. 64: 1580 1583.
122. Flett, F.,, V. Mersinias,, and C. P. Smith. 1997. High efficiency intergeneric conjugal transfer of plasmid DNA from Escherichia coli to methyl DNA-restricting streptomycetes. FEMS Microbiol. Lett. 155: 223 229.
123. Flint, J. L.,, J. C. Kowalski,, P. K. Karnati,, and K. M. Derbyshire. 2004. The RD1 virulence locus of Mycobacterium tuberculosis regulates DNA transfer in Mycobacterium smegmatis. Proc. Natl. Acad. Sci. USA 101: 12598 12603.
124. Fogg, G. C.,, and M. G. Caparon. 1997. Constitutive expression of fibronectin binding in Streptococcus pyogenes as a result of anaerobic activation of rofA. J. Bacteriol. 179: 6172 6180.
125. Forsman, M.,, and B. Jaurin. 1987. Chromogenic identification of promoters in Streptomyces lividans by using an ampC β-lactamase promoter-probe vector. Mol. Gen. Genet. 210: 23 32.
126. Francis, K. P.,, D. Joh,, C. Bellinger-Kawahara,, M. J. Hawkinson,, T. F. Purchio,, and P. R. Contag. 2000. Monitoring bioluminescent Staphylococcus aureus infections in living mice using a novel luxABCDE construct. Infect. Immun. 68: 3594 3600.
127. Francis, K. P.,, J. Yu,, C. Bellinger-Kawahara,, D. Joh,, M. J. Hawkinson,, G. Xiao,, T. F. Purchio,, M. G. Caparon,, M. Lipsitch,, and P. R. Contag. 2001. Visualizing pneumococcal infections in the lungs of live mice using bioluminescent Streptococcus pneumoniae transformed with a novel gram-positive lux transposon. Infect. Immun. 69: 3350 3358.
128. Franke, A. E.,, and D. B. Clewell. 1981. Evidence for a chromosome-borne resistance transposon (Tn 916) in Streptococcus faecalis that is capable of “conjugal” transfer in the absence of a conjugative plasmid. J. Bacteriol. 145: 494 502.
129. Frazier, C. L.,, J. San Filippo,, A. M. Lambowitz,, and D. A. Mills. 2003. Genetic manipulation of Lactococcus lactis by using targeted group II introns: generation of stable insertions without selection. Appl. Environ. Microbiol. 69: 1121 1128.
130. Freitag, N. E., 2000. Genetic tools for use with Listeria monocytogenes, p. 488 498. In V. A. Fischetti,, R. P. Novick,, J. J. Ferretti,, D. A. Portnoy,, and J. I. Rood (ed.), Gram-Positive Pathogens. ASM Press, Washington, DC.
131. Fu, X.,, and J. G. Xu. 2000. Development of a chromosome-plasmid balanced lethal system for Lactobacillus acidophilus with thyA gene as selective marker. Microbiol. Immunol. 44: 551 556.
132. Gandlur, S. M.,, L. Wei,, J. Levine,, J. Russell,, and P. Kaur. 2004. Membrane topology of the DrrB protein of the doxorubicin transporter of Streptomyces peucetius. J. Biol. Chem. 279: 27799 27806.
133. Garcia, P.,, A. C. Martin,, and R. Lopez. 1997. Bacteriophages of Streptococcus pneumoniae: a molecular approach. Microb. Drug Resist. 3: 165 176.
134. Garnier, T.,, and S. T. Cole. 1988. Complete nucleotide sequence and genetic organization of the bacteriocinogenic plasmid, pIP404, from Clostridium perfringens. Plasmid 19: 134 150.
135. Garsin, D. A.,, J. Urbach,, J. C. Huguet-Tapia,, J. E. Peters,, and F. M. Ausubel. 2004. Construction of an Enterococcus faecalis Tn 917-mediated-gene-disruption library offers insight into Tn 917 insertion patterns. J. Bacteriol. 186: 7280 7289.
136. Gasson, M.,, J.-J. Godon,, C. J. Pillidge,, T. J. Eaton,, K. L. Jury,, and C. A. Shearman. 1995. Characterization and exploitation of conjugation in Lactococcus lactis. Int. Dairy J. 5: 757 762.
137. Gasson, M. J. 1990. In vivo genetic systems in lactic acid bacteria. FEMS Microbiol. Rev. 7: 43 60.
138. Gawron-Burke, C.,, and D. B. Clewell. 1982. A transposon in Streptococcus faecalis with fertility properties. Nature 300: 281 284.
139. Gehring, A. M.,, J. R. Nodwell,, S. M. Beverley,, and R. Losick. 2000. Genomewide insertional mutagenesis in Streptomyces coelicolor reveals additional genes involved in morphological differentiation. Proc. Natl. Acad. Sci. USA 97: 9642 9647.
140. Geist, R. T.,, N. Okada,, and M. G. Caparon. 1993. Analysis of Streptococcus pyogenes promoters by using novel Tn 916-based shuttle vectors for the construction of transcriptional fusions to chloramphenicol acetyltransferase. J. Bacteriol. 175: 7561 7570.
141. Gibson, C. M.,, and M. G. Caparon. 2002. Alkaline phosphatase reporter transposon for identification of genes encoding secreted proteins in gram-positive microorganisms. Appl. Environ. Microbiol. 68: 928 932.
142. Gibson, E. M.,, N. M. Chace,, S. B. London,, and J. London. 1979. Transfer of plasmid-mediated antibiotic resistance from streptococci to lactobacilli. J. Bacteriol. 137: 614 619.
143. Gonzalez, C. F.,, and B. S. Kunka. 1983. Plasmid transfer in Pediococcus spp.: intergeneric and intrageneric transfer of pIP501. Appl. Environ. Microbiol. 46: 81 89.
144. Gormley, E. P.,, and J. Davies. 1991. Transfer of plasmid rsf1010 by conjugation from Escherichia coli to Streptomyces lividans and Mycobacterium smegmatis. J. Bacteriol. 173: 6705 6708.
145. Gory, L.,, M. C. Montel,, and M. Zagorec. 2001. Use of green fluorescent protein to monitor Lactobacillus sakei in fermented meat products. FEMS Microbiol. Lett. 194: 127 133.
146. Gosalbes, M. J.,, C. D. Esteban,, J. L. Galan,, and G. Perez-Martinez. 2000. Integrative food-grade expression system based on the lactose regulon of Lactobacillus casei. Appl. Environ. Microbiol. 66: 4822 4828.
147. Granok, A. B.,, D. Parsonage,, R. P. Ross,, and M. G. Caparon. 2000. The RofA binding site in Streptococcus pyogenes is utilized in multiple transcriptional pathways. J. Bacteriol. 182: 1529 1540.
148. Grant, R. B.,, and H. P. Riemann. 1976. Temperate phages of Clostridium perfringens type C1. Can. J. Microbiol. 22: 603 610.
149. Griffith, F. 1928. The significance of pneumococcal types. J. Hyg. (London) 27: 113 159.
150. Grohmann, E.,, G. Muth,, and M. Espinosa. 2003. Conjugative plasmid transfer in gram-positive bacteria. Microbiol. Mol. Biol. Rev. 67: 277 301.
151. Gryllos, I.,, C. Cywes,, M. H. Shearer,, M. Cary,, R. C. Kennedy,, and M. R. Wessels. 2001. Regulation of capsule gene expression by group A Streptococcus during pharyngeal colonization and invasive infection. Mol. Microbiol. 42: 61 74.
152. Guerout-Fleury, A. M.,, N. Frandsen,, and P. Stragier. 1996. Plasmids for ectopic integration in Bacillus subtilis. Gene 180: 57 61.
153. Gust, B.,, G. L. Challis,, K. Fowler,, T. Kieser,, and K. F. Chater. 2003. PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc. Natl. Acad. Sci. USA 100: 1541 1546.
154. Hamoen, L. W.,, G. Venema,, and O. P. Kuipers. 2003. Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology 149: 9 17.
155. Hashiba, H.,, R. Takiguchi,, K. Jyoho,, and K. Aoyama. 1992. Establishment of a host-vector system in Lactobacillus helveticus with beta-galactosidase activity as a selection marker. Biosci. Biotechnol. Biochem. 56: 190 194.
156. Hatfull, G. F. 1993. Genetic transformation of mycobacteria. Trends Microbiol. 1: 310 314.
157. Hatfull, G. F. 1996. The molecular genetics of Mycobacterium tuberculosis. Curr. Top. Microbiol. Immunol. 215: 29 47.
158. Hatfull, G. F., 2000. Molecular genetics of mycobacteriophages, p. 37 54. In G. F. Hatfull, and W. R. Jacobs, Jr. (ed.), Molecular Genetics of Mycobacteria. ASM Press, Washington, DC.
159. Hava, D. L.,, and A. Camilli. 2002. Large-scale identification of serotype 4 Streptococcus pneumoniae virulence factors. Mol. Microbiol. 45: 1389 1406.
160. Heffron, F.,, C. Rubens,, and S. Falkow. 1975. Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion. Proc. Natl. Acad. Sci. USA 72: 3623 3627.
161. Helmark, S.,, M. E. Hansen,, B. Jelle,, K. I. Sorensen,, and P. R. Jensen. 2004. Transformation of Leuconostoc carnosum 4010 and evidence for natural competence of the organism. Appl. Environ. Microbiol. 70: 3695 3699.
162. Henderson, I.,, T. Davis,, M. Elmore,, and N. Minton,. 1997. The genetic basis of toxin production in Clostridium botulinum and Clostridium tetani, p. 261 294. In J. I. Rood,, B. A. McClane,, J. G. Songer,, and R. W. Titball (ed.), The Clostridia: Molecular Biology and Pathogenesis. Academic Press, Inc., London, United Kingdom.
163. Hendrix, R. W. 2003. Bacteriophage genomics. Curr. Opin. Microbiol. 6: 506 511.
164. Hendrix, R. W.,, M. C. Smith,, R. N. Burns,, M. E. Ford,, and G. F. Hatfull. 1999. Evolutionary relationships among diverse bacteriophages and prophages: all the world’s a phage. Proc. Natl. Acad. Sci. USA 96: 2192 2197.
165. Henrich, B.,, J. R. Klein,, B. Weber,, C. Delorme,, P. Renault,, and U. Wegmann. 2002. Food-grade delivery system for controlled gene expression in Lactococcus lactis. Appl. Environ. Microbiol. 68: 5429 5436.
166. Hickey, R. M.,, D. P. Twomey,, R. P. Ross,, and C. Hill. 2001. Exploitation of plasmid pMRC01 to direct transfer of mobilizable plasmids into commercial lactococcal starter strains. Appl. Environ. Microbiol. 67: 2853 2858.
167. Hillemann, D.,, A. Puhler,, and W. Wohlleben. 1991. Gene disruption and gene replacement in Streptomyces via single-stranded DNA transformation of integration vectors. Nucleic Acids Res. 19: 727 731.
168. Holo, H.,, and I. F. Nes. 1995. Transformation of Lactococcus by electroporation. Methods Mol. Biol. 47: 195 199.
169. Horinouchi, S.,, and T. Beppu. 1985. Construction and application of a promoter-probe plasmid that allows chromogenic identification in Streptomyces lividans. J. Bacteriol. 162: 406 412.
170. Horodniceanu, T.,, D. H. Bouanchaud,, G. Bieth,, and Y. A. Chabbert. 1976. R plasmids in Streptococcus agalactiae (group B). Antimicrob. Agents Chemother. 10: 795 801.
171. Hosted, T. J.,, and R. H. Baltz. 1997. Use of rpsL for dominance selection and gene replacement in Streptomyces roseosporus. J. Bacteriol. 179: 180 186.
171a.. Hu, X.,, B. M. Hansen,, J. Eilenberg,, N. B. Hendriksen,, L. Smidt,, Z. Yuan,, and G. B. Jensen. 2004. Conjugative transfer, stability and expression of a plasmid encoding a cry1Ac gene in Bacillus cereus group strains. FEMS Microbiol. Lett. 231: 45 52.
172. Hu, Z. H.,, D. Hunziker,, C. R. Hutchinson,, and C. Khosla. 1999. A host-vector system for analysis and manipulation of rifamycin polyketide biosynthesis in Amycolatopsis mediterranei. Microbiology 145: 2335 2341.
173. Hutter, B.,, C. Fischer,, A. Jacobi,, C. Schaab,, and H. Loferer. 2004. Panel of Bacillus subtilis reporter strains indicative of various modes of action. Antimicrob. Agents Chemother. 48: 2588 2594.
174. Ike, Y.,, K. Tanimoto,, H. Tomita,, K. Takeuchi,, and S. Fujimoto. 1998. Efficient transfer of the pheromone-independent Enterococcus faecium plasmid pMG1 (Gmr) (65.1 kilobases) to Enterococcus strains during broth mating. J. Bacteriol. 180: 4886 4892.
175. Ikeda, H.,, Y. Takada,, C. H. Pang,, H. Tanaka,, and S. Omura. 1993. Transposon mutagenesis by Tn 4560 and applications with avermectin-producing Streptomyces avermitilis. J. Bacteriol. 175: 2077 2082.
176. Ince, D.,, and D. C. Hooper. 2000. Mechanisms and frequency of resistance to premafloxacin in Staphylococcus aureus: novel mutations suggest novel drug-target interactions. Antimicrob. Agents Chemother. 44: 3344 3350.
177. Ingavale, S. S.,, W. Van Wamel,, and A. L. Cheung. 2003. Characterization of RAT, an autolysis regulator in Staphylococcus aureus. Mol. Microbiol. 48: 1451 1466.
178. Ingram, C.,, M. Brawner,, P. Youngman,, and J. Westpheling. 1989. xylE functions as an efficient reporter gene in Streptomyces spp: use for the study of galP1, a catabolitecontrolled promoter. J. Bacteriol. 171: 6617 6624.
179. Israelsen, H.,, S. M. Madsen,, A. Vrang,, E. B. Hansen,, and E. Johansen. 1995. Cloning and partial characterization of regulated promoters from Lactococcus lactis Tn 917- lacZ integrants with the new promoter probe vector, pAK80. Appl. Environ. Microbiol. 61: 2540 2547.
180. Jacobs, W. R., Jr.,, G. V. Kalpana,, J. D. Cirillo,, L. Pascopella,, S. B. Snapper,, R. A. Udani,, W. Jones,, R. G. Barletta,, and B. R. Bloom. 1991. Genetic systems for mycobacteria. Methods Enzymol. 204: 537 555.
181. Janniere, L.,, A. Gruss,, and D. Ehrlich,. 1993. Plasmids, p. 625 644. In A. L. Sonenshein,, J. A. Hoch,, and R. Losick (ed.), Bacillus subtilis and other Gram-Positive Bacteria: Biochemistry, Physiology, and Molecular Genetics. American Society for Microbiology, Washington, DC.
182. Jensen, P. R.,, and K. Hammer. 1998. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Appl. Environ. Microbiol. 64: 82 87.
183. Ji, Y.,, A. Marra,, M. Rosenberg,, and G. Woodnutt. 1999. Regulated antisense RNA eliminates alpha-toxin virulence in Staphylococcus aureus infection. J. Bacteriol. 181: 6585 6590.
184. Ji, Y.,, L. McLandsborough,, A. Kondagunta,, and P. P. Cleary. 1996. C5a peptidase alters clearance and trafficking of group A streptococci by infected mice. Infect. Immun. 64: 503 510.
185. Johnson, E., 1997. Extrachromosomal virulence determinants in the clostridia, p. 35 48. In J. I. Rood,, B. A. McClane,, J. G. Songer,, and R. W. Titball (ed.), The Clostridia: Molecular Biology and Pathogenesis. Academic Press, Inc., London, United Kingdom.
186. Johnson, L. P.,, and P. M. Schlievert. 1984. Group A streptococcal phage T12 carries the structural gene for pyrogenic exotoxin type A. Mol. Gen. Genet. 194: 52 56.
187. Johnson, L. P.,, P. M. Schlievert,, and D. W. Watson. 1980. Transfer of group A streptococcal pyrogenic exotoxin production to nontoxigenic strains of lysogenic conversion. Infect. Immun. 28: 254 257.
188. Jones, J. M.,, S. C. Yost,, and P. A. Pattee. 1987. Transfer of the conjugal tetracycline resistance transposon Tn 916 from Streptococcus faecalis to Staphylococcus aureus and identification of some insertion sites in the staphylococcal chromosome. J. Bacteriol. 169: 2121 2131.
189. Kadioglu, A.,, J. A. Sharpe,, I. Lazou,, C. Svanborg,, C. Ockleford,, T. J. Mitchell,, and P. W. Andrew. 2001. Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo. FEMS Microbiol. Lett. 194: 105 110.
190. Katayama, Y.,, T. Ito,, and K. Hiramatsu. 2000. A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 44: 1549 1555.
191. Kendall, K. J.,, and S. N. Cohen. 1987. Plasmid transfer in Streptomyces lividans: identification of a kil- kor system associated with the transfer region of pIJ101. J. Bacteriol. 169: 4177 4183.
192. Kendall, K. J.,, and S. N. Cohen. 1988. Complete nucleotide sequence of the Streptomyces lividans plasmid pIJ101 and correlation of the sequence with genetic properties. J. Bacteriol. 170: 4634 4651.
193. Khan, S. A. 1997. Rolling-circle replication of bacterial plasmids. Microbiol. Mol. Biol. Rev. 61: 442 455.
194. Khan, S. A. 2000. Plasmid rolling-circle replication: recent developments. Mol. Microbiol. 37: 477 484.
195. Khosla, C.,, S. Ebertkhosla,, and D. A. Hopwood. 1992. Targeted gene replacements in a Streptomyces polyketide synthase gene cluster: role for the acyl carrier protein. Mol. Microbiol. 6: 3237 3249.
196. Khosla, C.,, R. McDaniel,, S. Ebertkhosla,, R. Torres,, D. H. Sherman,, M. J. Bibb,, and D. A. Hopwood. 1993. Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins. J. Bacteriol. 175: 2197 2204.
197. Kieser, T.,, M. J. Bibb,, M. J. Buttner,, K. F. Chater,, and D. A. Hopwood. 2000. Practical Streptomyces Genetics. The John Innes Foundation, Norwich, England.
198. Kieser, T.,, D. A. Hopwood,, H. M. Wright,, and C. J. Thompson. 1982. pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Mol. Gen. Genet. 185: 223 238.
199. Kieser, T.,, and R. E. Melton. 1988. Plasmid pIJ699, a multi-copy positive-selection vector for Streptomyces. Gene 65: 83 91.
200. Kim, B. S.,, D. H. Sherman,, and K. A. Reynolds. 2004. An efficient method for creation and functional analysis of libraries of hybrid type I polyketide synthases. Protein Eng. Des. Sel. 17: 277 284.
201. Kim, E. S.,, D. A. Hopwood,, and D. H. Sherman. 1994. Analysis of type-II polyketide β-ketoacyl synthase specificity in Streptomyces coelicolor A3(2) by trans complementation of actinorhodin synthase mutants. J. Bacteriol. 176: 1801 1804.
202. Kinashi, H.,, M. Shimaji,, and A. Sakai. 1987. Giant linear plasmids in Streptomyces which code for antibiotic biosynthesis genes. Nature 328: 454 456.
203. Kirby, C.,, A. Waring,, T. J. Griffin,, J. O. Falkinham III,, N. D. Grindley,, and K. M. Derbyshire. 2002. Cryptic plasmids of Mycobacterium avium: Tn 552 to the rescue. Mol. Microbiol. 43: 173 186.
204. Klaenhammer, T. R.,, and R. B. Sanozky. 1985. Conjugal transfer from Streptococcus lactis ME2 of plasmids encoding phage resistance, nisin resistance and lactose-fermenting ability: evidence for a high-frequency conjugative plasmid responsible for abortive infection of virulent bacteriophage. J. Gen. Microbiol. 131: 1531 1541.
205. Kleerebezem, M.,, M. M. Beerthuyzen,, E. E. Vaughan,, W. M. de Vos,, and O. P. Kuipers. 1997. Controlled gene expression systems for lactic acid bacteria: transferable nisin-inducible expression cassettes for Lactococcus, Leuconostoc, and Lactobacillus spp. Appl. Environ. Microbiol. 63: 4581 4584.
206. Koehler, T. M.,, Z. Dai,, and M. Kaufman-Yarbray. 1994. Regulation of the Bacillus anthracis protective antigen gene: CO 2 and a trans-acting element activate transcription from one of two promoters. J. Bacteriol. 176: 586 595.
207. Kok, J.,, J. M. van der Vossen,, and G. Venema. 1984. Construction of plasmid cloning vectors for lactic streptococci which also replicate in Bacillus subtilis and Escherichia coli. Appl. Environ. Microbiol. 48: 726 731.
208. Kondo, I.,, and K. Fujise. 1977. Serotype B staphylococcal bacteriophage singly converting staphylokinase. Infect. Immun. 18: 266 272.
209. Konings, W. N.,, J. Kok,, O. P. Kuipers,, and B. Poolman. 2000. Lactic acid bacteria: the bugs of the new millennium. Curr. Opin. Microbiol. 3: 276 282.
210. Kreikemeyer, B.,, M. D. Boyle,, B. A. Buttaro,, M. Heinemann,, and A. Podbielski. 2001. Group A streptococcal growth phase-associated virulence factor regulation by a novel operon (Fas) with homologies to twocomponent- type regulators requires a small RNA molecule. Mol. Microbiol. 39: 392 406.
210a.. Kristich, C. J.,, J. R. Chandler,, and G. M. Dunny. 2007. Development of a host-genotype-independent counterselectable marker and a high-frequency conjugative delivery system and their use in genetic analysis of Enterococcus faecalis. Plasmid 57: 133 144.
211. Kristich, C. J.,, D. A. Manias,, and G. M. Dunny. 2005. Development of a method for markerless genetic exchange in Enterococcus faecalis and its use in construction of a srtA mutant. Appl. Environ. Microbiol. 71: 5837 5849.
212. Kuipers, O. P.,, H. S. Rollema,, R. J. Siezen,, and W. M. De Vos. 1995. Lactococcal expression systems for protein engineering of nisin. Dev. Biol. Stand. 85: 605 613.
213. Kurenbach, B.,, C. Bohn,, J. Prabhu,, M. Abudukerim,, U. Szewzyk,, and E. Grohmann. 2003. Intergeneric transfer of the Enterococcus faecalis plasmid pIP501 to Escherichia coli and Streptomyces lividans and sequence analysis of its tra region. Plasmid 50: 86 93.
214. Kuroda, M.,, T. Ohta,, I. Uchiyama,, T. Baba,, H. Yuzawa,, I. Kobayashi,, L. Cui,, A. Oguchi,, K. Aoki,, Y. Nagai,, J. Lian,, T. Ito,, M. Kanamori,, H. Matsumaru,, A. Maruyama,, H. Murakami,, A. Hosoyama,, Y. Mizutani- Ui,, N. K. Takahashi,, T. Sawano,, R. Inoue,, C. Kaito,, K. Sekimizu,, H. Hirakawa,, S. Kuhara,, S. Goto,, J. Yabuzaki,, M. Kanehisa,, A. Yamashita,, K. Oshima,, K. Furuya,, C. Yoshino,, T. Shiba,, M. Hattori,, N. Ogasawara,, H. Hayashi,, and K. Hiramatsu. 2001. Whole genome sequencing of meticillin-resistant Staphylococcus aureus. Lancet 357: 1225 1240.
215. Landman, O. E.,, D. J. Badkin,, C. W. J. Finn,, and R. A. Pepin. 1980. Presented at the Proceedings of the Fifth European Meeting on Bacterial Transformation and Transfection.
216. Langella, P.,, and A. Chopin. 1989. Effect of restrictionmodification systems on transfer of foreign DNA into Lactococcus lactis subsp. lactis. FEMS Microbiol. Lett. 59: 301 306.
217. Lau, P. C.,, C. K. Sung,, J. H. Lee,, D. A. Morrison,, and D. G. Cvitkovitch. 2002. PCR ligation mutagenesis in transformable streptococci: application and efficiency. J. Microbiol. Methods 49: 193 205.
218. Law, J.,, G. Buist,, A. Haandrikman,, J. Kok,, G. Venema,, and K. Leenhouts. 1995. A system to generate chromosomal mutations in Lactococcus lactis which allows fast analysis of targeted genes. J. Bacteriol. 177: 7011 7018.
219. LeBlanc, D. J.,, L. N. Lee,, and A. Abu-Al-Jaibat. 1992. Molecular, genetic, and functional analysis of the basic replicon of pVA380-1, a plasmid of oral streptococcal origin. Plasmid 28: 130 145.
219a.. Le Breton, Y.,, N. P. Mohapatra,, and W. G. Haldenwang. 2006. In vivo random mutagenesis of Bacillus subtilis by use of TnYLB-1, a mariner-based transposon. Appl. Environ. Microbiol. 72: 327 333.
220. Lederberg, J. 1951. Streptomycin resistance: a genetically recessive mutation. J. Bacteriol. 61: 549 550.
221. Lee, C. Y.,, S. L. Buranen,, and Z. H. Ye. 1991. Construction of single-copy integration vectors for Staphylococcus aureus. Gene 103: 101 105.
222. Lee, C. Y.,, and J. J. Iandolo. 1986. Lysogenic conversion of staphylococcal lipase is caused by insertion of the bacteriophage L54a genome into the lipase structural gene. J. Bacteriol. 166: 385 391.
223. Lee, M. H.,, A. Nittayajarn,, R. P. Ross,, C. B. Rothschild,, D. Parsonage,, A. Claiborne,, and C. E. Rubens. 1999. Characterization of Enterococcus faecalis alkaline phosphatase and use in identifying Streptococcus agalactiae secreted proteins. J. Bacteriol. 181: 5790 5799.
224. Lee, M. S.,, B. A. Dougherty,, A. C. Madeo,, and D. A. Morrison. 1999. Construction and analysis of a library for random insertional mutagenesis in Streptococcus pneumoniae: use for recovery of mutants defective in genetic transformation and for identification of essential genes. Appl. Environ. Microbiol. 65: 1883 1890.
225. Lee, M. S.,, C. Seok,, and D. A. Morrison. 1998. Insertion-duplication mutagenesis in Streptococcus pneumoniae: targeting fragment length is a critical parameter in use as a random insertion tool. Appl. Environ. Microbiol. 64: 4796 4802.
226. Leelaporn, A.,, N. Firth,, I. T. Paulsen,, and R. A. Skurray. 1996. IS 257-mediated cointegration in the evolution of a family of staphylococcal trimethoprim resistance plasmids. J. Bacteriol. 178: 6070 6073.
227. Leenhouts, K. J.,, B. Tolner,, S. Bron,, J. Kok,, G. Venema,, and J. F. Seegers. 1991. Nucleotide sequence and characterization of the broad-host-range lactococcal plasmid pWVO1. Plasmid 26: 55 66.
228. Lewis, P. J.,, and A. L. Marston. 1999. GFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis. Gene 227: 101 110.
229. Lin, M. Y.,, S. Harlander,, and D. Savaiano. 1996. Construction of an integrative food-grade cloning vector for Lactobacillus acidophilus. Appl. Microbiol. Biotechnol. 45: 484 489.
230. Lin, W. S.,, T. Cunneen,, and C. Y. Lee. 1994. Sequence analysis and molecular characterization of genes required for the biosynthesis of type 1 capsular polysaccharide in Staphylococcus aureus. J. Bacteriol. 176: 7005 7016.
231. Lithgow, J. K.,, E. Ingham,, and S. J. Foster. 2004. Role of the hprT-ftsH locus in Staphylococcus aureus. Microbiology 150: 373 381.
232. Liu, C. Q.,, P. Su,, N. Khunajakr,, Y. M. Deng,, S. Sumual,, W. S. Kim,, J. E. Tandianus,, and N. W. Dunn. 2005. Development of food-grade cloning and expression vectors for Lactococcus lactis. J. Appl. Microbiol. 98: 127 135.
233. Liu, S.,, S. Sela,, G. Cohen,, J. Jadoun,, A. Cheung,, and I. Ofek. 1997. Insertional inactivation of streptolysin S expression is associated with altered riboflavin metabolism in Streptococcus pyogenes. Microb. Pathog. 22: 227 234.
234. Löfdahl, S.,, J. E. Sjöström,, and L. Philipson. 1981. Cloning of restriction fragments of DNA from staphylococcal bacteriophage ϕ11. J. Virol. 37: 795 801.
235. Lopez, R. 2004. Streptococcus pneumoniae and its bacteriophages: one long argument. Int. Microbiol. 7: 163 171.
236. Luchansky, J. B.,, E. G. Kleeman,, R. R. Raya,, and T. R. Klaenhammer. 1989. Genetic transfer systems for delivery of plasmid deoxyribonucleic acid to Lactobacillus acidophilus ADH: conjugation, electroporation, and transduction. J. Dairy Sci. 72: 1408 1417.
237. Luchansky, J. B.,, P. M. Muriana,, and T. R. Klaenhammer. 1988. Application of electroporation for transfer of plasmid DNA to Lactobacillus, Lactococcus, Leuconostoc, Listeria, Pediococcus, Bacillus, Staphylococcus, Enterococcus and Propionibacterium. Mol. Microbiol. 2: 637 646.
238. Luo, P.,, H. Li,, and D. A. Morrison. 2003. ComX is a unique link between multiple quorum sensing outputs and competence in Streptococcus pneumoniae. Mol. Microbiol. 50: 623 633.
239. Luo, P.,, H. Li,, and D. A. Morrison. 2004. Identification of ComW as a new component in the regulation of genetic transformation in Streptococcus pneumoniae. Mol. Microbiol. 54: 172 183.
240. Lydiate, D. J.,, F. Malpartida,, and D. A. Hopwood. 1985. The Streptomyces plasmid SCP2*: its functional analysis and development into useful cloning vectors. Gene 35: 223 235.
241. Lyon, W. R.,, C. M. Gibson,, and M. G. Caparon. 1998. A role for trigger factor and an rgg-like regulator in the transcription, secretion and processing of the cysteine proteinase of Streptococcus pyogenes. EMBO J. 17: 6263 6275.
242. Lyras, D.,, and J. I. Rood. 1998. Conjugative transfer of RP4- oriT shuttle vectors from Escherichia coli to Clostridium perfringens. Plasmid 39: 160 164.
243. Lyras, D.,, and J. I. Rood,. 2000. Clostridial genetics, p. 529 539. In V. A. Fischetti,, R. P. Novick,, J. J. Ferretti,, D. A. Portnoy,, and J. I. Rood (ed.), Gram-Positive Pathogens. ASM Press, Washington, DC.
244. Maas, R.-M.,, J. Gotz,, W. Wohlleben,, and G. Muth. 1998. The conjugative plasmid pSG5 from Streptomyces ghanaensis DSM 2932 differs in its transfer functions from other Streptomyces rolling-circle-type plasmids. Microbiology 144: 2809 2817.
245. Macneil, D. J. 1987. Introduction of plasmid DNA into Streptomyces lividans by electroporation. FEMS Microbiol. Lett. 42: 239 244.
246. Macneil, D. J.,, K. M. Gewain,, C. L. Ruby,, G. Dezeny,, P. H. Gibbons,, and T. Macneil. 1992. Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector. Gene 111: 61 68.