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Chapter 43 : Physiology, Metabolism, and Molecular Aspects of Filamentous Fungi

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Abstract:

The filamentous fungi display remarkable and diverse metabolic pathways and show great versatility in the utilization of sources of carbon, nitrogen, phosphorus, sulfur, and other metabolites and in acquiring essential elements, e.g., iron and potassium. This chapter emphasizes practical aspects, methods, and simple assays for investigating fungal physiology and metabolism and introduces some of the specialized molecular techniques that have proved invaluable in studying this fascinating group of eukaryotic microorganisms. In studies of metabolism and gene expression, it is often necessary to examine RNA and protein synthesis. A number of useful methods for RNA isolation from filamentous fungi have been devised. The ability to readily transform filamentous fungi with exogenous DNA has been revolutionary and provided the essential step required in cloning, characterization, and sophisticated manipulation of individual genes. The fungi have many remarkable characteristics and promise a wealth of exciting new phenomena and discoveries as their biology and genetics are further explored.

Citation: Marzluf G. 2007. Physiology, Metabolism, and Molecular Aspects of Filamentous Fungi, p 952-964. In Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L (ed), Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817497.ch43

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Enzymes and Coenzymes
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Neurospora crassa
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Protein Synthesis RNAs
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Cellular Processes
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References

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Tables

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TABLE 1

GATA factors of filamentous fungi

+, positive regulation; −, negative regulation.

Citation: Marzluf G. 2007. Physiology, Metabolism, and Molecular Aspects of Filamentous Fungi, p 952-964. In Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L (ed), Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817497.ch43
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TABLE 2

Pathway-specific regulatory factors which mediate induction of catabolic genes in filamentous fungi

Citation: Marzluf G. 2007. Physiology, Metabolism, and Molecular Aspects of Filamentous Fungi, p 952-964. In Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L (ed), Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817497.ch43
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TABLE 3

and genes which function in nitrate assimilation

Wild-type strains grown on all of these nitrogen sources. Wild-type and all mutant strains grow using glutamine as the sole nitrogen source. Nitrate transporter mutants (and mutants) cannot grow with low concentrations of nitrate.

Citation: Marzluf G. 2007. Physiology, Metabolism, and Molecular Aspects of Filamentous Fungi, p 952-964. In Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L (ed), Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817497.ch43

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