1887

Color Plates

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.
  • XML
    20.58 Kb
  • HTML
    23.01 Kb
Add to My Favorites
You must be logged in to use this functionality
Loading full text...

Full text loading...

/deliver/fulltext/10.1128/9781555817497/cplates.html?itemId=/content/book/10.1128/9781555817497.cplates&mimeType=html&fmt=ahah

Figures

Image of COLOR PLATE 1 (chapter 2)

Click to view

COLOR PLATE 1 (chapter 2)

Dually fluorescently labeled cells where the DNA has been stained with DAPI (blue) and the P1 adhesion protein has been stained with anti-P1/Alexa 488 (red) to show their respective cellular positions. Scale bar, 1 μm. (Reprinted from reference 48 with the authors' permission.)

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 2 (chapter 2)

Click to view

COLOR PLATE 2 (chapter 2)

Gray-scale image of negatively stained with India ink. The spiral shape of this gram-negative bacterium can be seen as well as black aggregates of ink. Scale bar, 5 μm. (Kindly prepared and supplied by R. Van Twest, University of Guelph, Guelph, Ontario, Canada.)

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 3 (chapter 2)

Click to view

COLOR PLATE 3 (chapter 2)

Gram stain of that has been incubated with egg white lysozyme to digest the peptidoglycan in the cell wall. Some cells have lysed and therefore stain as gram negative (red), whereas others remain intact and stain as gram positive (purple). Scale bar, 5 μm. (Kindly prepared and supplied by R. Van Twest.)

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 4 (chapter 3)

Click to view

COLOR PLATE 4 (chapter 3)

CLSM images of 6-μm-diameter Focal Check beads stained with green, red, and blue fluors. (A) Beads imaged with a 60×, 1.4-NA lens. The image shows a white ring indicating good alignment of the LSM and correction of the lens. (B) Loss of resolution in the plane due to light wavelength and point spread function error. (C) Effect of using a 63×, 0.9-NA lens on the appearance of the same beads in the plane. (D) Further reduced quality of the image (dashed lines indicate location of image). (E) Effect of imaging with the outer edge of the 63× lens. (F) Loss of confocality (presence of multiple planes and bead colors) and chromatic correction in a 20×, 0.4-NA lens.

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 5 (chapter 3)

Click to view

COLOR PLATE 5 (chapter 3)

(A) Three-channel 1P-LSM Z-series projected as a stereo pair showing the colocalization of binding sites for three lectins, -TRITC (red), -FITC (green), and -Cy5 (blue), in a river biofilm. A variety of microcolonies and individual cells can be seen to bind single or combinations of the lectins as indicated by the color coding of the area. (B) High-resolution imaging of a microcolony stained with the lectin conjugates -FITC (green), -Alexa568 (red), and -Cy5 (blue). (C) -FITC (green), -TRITC (red), and -Cy5 (blue). These three-channel images show the details of binding and structure of the multicomponent polymer surrounding individual bacterial cells in a microcolony. (A color wheel is included to allow interpretation of stain combinations.)

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 6 (chapter 3)

Click to view

COLOR PLATE 6 (chapter 3)

Multiparameter image showing the distribution of bacteria (green), algae (autofluorescence red), and exopolymeric substances stained with lectin (blue). The image stack was projected with one version of the stack offset and aligned to form a stereo pair.

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 7 (chapter 4)

Click to view

COLOR PLATE 7 (chapter 4)

An example of the use of ESI, TEM, and three-dimensional reconstruction (see chapter 5) to determine the location of rRNA (by following the phosphorus line) on the surface of the small (30S) ribosomal subunit of . A 150- ±9-eV loss (green) and NetP (orange) are shown in four views which are related to one another by 90° rotations about the long axis of the subunit. In panel a the reconstructions are represented as solid surfaces, in panel b only the NetP reconstructions are shown, and in panel c both reconstructions are shown as wire mesh models. Spheres with numbers indicate positions of proteins mapped by neutron diffraction in studies by other researchers. In the first column of images, the intersubunit face of the small subunit is facing the viewer. The bottom of the subunit is shown to be phosphorus rich (i.e., rRNA rich). (From D. R. Beniac, G. J. Czarnota, B. L. Rutherford, F. P. Ottensmeyer, and G. Harauz, . 188:24–35, 1997.)

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 8 (chapter 5)

Click to view

COLOR PLATE 8 (chapter 5)

As described in reference 23, an atomic model of the phosphoenolpyruvate synthase subunit was constructed by using Insight software (i) and 24 subunits were computationally matched to the 3-D reconstruction in Fig. 8d. Here, the view down the three-fold axis of rotational symmetry shows the enzymatically active sites of each subunit clustered around a putative channel. This figure was provided by F. P. Ottensmeyer, Ontario Cancer Institute, Toronto, Canada.

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 9 (chapter 16)

Click to view

COLOR PLATE 9 (chapter 16)

Diversity of symbiosis. (A) Anaerobic methane-oxidizing consortium (FISH image; red, archaeal group; green, sulfate-reducing bacteria (credit: V. Orphan, Caltech). Scale bar, 10 μm. (B) Nematode () with epibionts (credit: M. Polz, MIT, and M. Bright, University of Vienna). Scale bar, 100 μm. (C) tube worms living near a deep-sea hydrothermal vent (credit: S. Goffredi, Monterey Bay Aquarium Research Institute). (D)Termite, (credit: Amy Vu, Caltech). (E) Hawaiian bobtail squid, -containing light organ is located on the ventral side of mantle cavity (not shown) (credit: M. Mcfall-Ngai, University of Wisconsin at Madison). (F) GFP- and dsRed-labeled inside infection threads of alfalfa. Clonal selection during the development of the root nodule is evident due to distinct GFP and dsRed- in each infection thread. Scale bar, 50 μm. [credit: D. Gage, University of Connecticut (61)]. (G) GFP-labeled in the intestine of an infective juvenile nematode, . The nematode and bacteria cooperate in insect pathogenesis (credit: T. Ciche, Michigan State University), (H) The gutless marine annelid (credit: A. Blazejak, Max Planck Institute).

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 10 (chapter 16)

Click to view

COLOR PLATE 10 (chapter 16)

(A) Transmission electron micrograph of the symbiont-containing region of . Smaller and larger arrows indicate smaller and larger symbiont morphotypes, respectively. Scale bar, 2 μm. cu, cuticle (44). (B) In situ epifluorescence identification of bacterial symbionts in . Cross sections through the entire worm. Dual hybridization with the GAM42a and DSS658/DSR651 probes, showing γ-proteobacterial symbionts (red) and δ-proteobacterial symbionts (green). Scale bar, 20 (μm (12).

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 11 (chapter 16)

Click to view

COLOR PLATE 11 (chapter 16)

mRNA FISH on gill filament of the mussel . Blue, thiotrophic symbiont labeled with a specific 16S rRNA probe; red, methanotrophic symbiont labeled with a specific 16S rRNA probe; green, activity of particulate methane monooxygenase of the symbiont (subunit A; ), a key enzyme of aerobic methane oxidation (credit: Annelie Pernthaler, Max Planck Institute).

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 12 (chapter 27)

Click to view

COLOR PLATE 12 (chapter 27)

DNA sequence chromatogram tracings. Screen shots were taken during the running of Sequencher software (Gene Codes Corp., Ann Arbor, MI). The sequence identified by the computer program is given above each tracing. (A) Normal sequence; (B) double peaks from a mixed clone; (C) failure with dirty template; (D) heterozygote template; (E) failure with high salt contamination; (F) blockage possibly due to hairpin formation.

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 13 (chapter 33)

Click to view

COLOR PLATE 13 (chapter 33)

sp. NRC-1. (A) Colonies of the model haloarchaeal strain sp. NRC-1. Among pink wild-type (NRC-1) colonies, an orange gas vesicle-minus (Vac) mutant is visible. (B) Liquid cultures of NRC-1 (tube 1), Vac mutant SD109 (tube 2), and a SD109 transformant containing the gas vesicle gene cluster on pFL2 (tube 3). Vac floating cells at the top of tubes 1 and 3 and Vac nonfloating cells at the bottom of tube 2 are visible. The meniscus is visible at the top of tube 2.

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 14 (chapter 39)

Click to view

COLOR PLATE 14 (chapter 39)

General protocol of whole-cell fluorescence in situ hybridization.

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 15 (chapter 39)

Click to view

COLOR PLATE 15 (chapter 39)

In situ accessibility of the 16S rRNA of (), sp. strain 1 (), (Archaea), and () for fluorescently labeled oligonucleotide probes. Different colors indicate differences in accessibility. From reference 8 with permission.

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 16 (chapter 39)

Click to view

COLOR PLATE 16 (chapter 39)

In situ accessibility of the 23S rRNA of . Different colors indicate differences in accessibility for oligonucleotide probes. From reference 17 with permission.

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 17 (chapter 41)

Click to view

COLOR PLATE 17 (chapter 41)

An example of analysis of T-RFLP data from three soils. Each soil type (H, N, and T) is presented with four replicates. (A) Aligned profiles from 12 samples taken into Excel™. Each profile includes the estimated size for each detected fragment. The checks indicate that a fragment was detected by visual inspection of the electropherogram but was not scored by the software because the peak amplitude value was below the preset limit. These are recorded as “present.” (B) The data set from panel A converted into binary format (presence or absence) for similarity comparisons. (C) Similarity analysis of the 12 samples with the T-RFLP function of the RDP. (D) UPGMA analysis of the 12 samples with PAUP (http://paup.csit.fsu.edu/).

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of COLOR PLATE 18 (chapter 44)

Click to view

COLOR PLATE 18 (chapter 44)

(Top left) Replica plating block used for replication of colonies. (Top right) cleistothecia appearing on mycelium near the mycelial frontier. (Bottom left) cleistothecia. The needle shaft is about 1 mm wide. The cleistothecium to its immediate left is uncleaned, with adhering Hülle cells and other debris. The cleistothecium to the far left has been cleaned by rolling on the agar. (Bottom right) Multipin replicator (to the right) used for rapid transfer of arrays of colonies. The colonies on the upper plate have grown after spreading conidia. Colonies are sampled individually from this plate and grown in the array shown in the lower plate. This will be the master plate used for transfers made with the multipin replicator.

Citation: Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T, Snyder L. 2007. Color Plates, In Methods for General and Molecular Microbiology, Third Edition. ASM Press, Washington, DC.
Permissions and Reprints Request Permissions
Download as Powerpoint

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error