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Chapter 18 : Ribosomes and Opportunities for Drug Intervention

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Abstract:

The translation of mRNA, a fundamental property of all organisms, is carried out by the ribosome. Although the ribosomes of sp. are typically eukaryotic in their sedimentation properties, they do differ from the host by the rRNA having a low G+C base composition. Indeed, antibiotics that selectively disable protein synthesis over defined periods of the developmental cycle or organellar function could play an important role in a better understanding of the molecular events during the developmental cycle of the malaria parasite. Our most detailed knowledge of ribosomal chemistry comes from the study of prokaryotic ribosomes. Within the ribosomal complex lies the machinery that decodes information from the messenger RNA and catalyzes the ordered assembly of amino acids into proteins. Regulation of ribosome production is essential to all cells. The number of ribosomes present in a cell is directly related to the protein synthesizing activity and to the size of the cell. The crystal structure of and 70S ribosome, along with biochemical data, suggests that ~80% of intersubunit bridges are contributed by RNA-RNA interactions. The functional role of the pseudoknot varies considerably depending on its source. The development and spread of drug resistance are unquestionably tied to the population dynamics of parasite, host, and vector.

Citation: Sharma I, McCutchan T. 2005. Ribosomes and Opportunities for Drug Intervention, p 353-364. In Sherman I (ed), Molecular Approaches to Malaria. ASM Press, Washington, DC. doi: 10.1128/9781555817558.ch18

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Bacterial Proteins
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Gene Expression and Regulation
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Figures

Image of FIGURE 1
FIGURE 1

Life cycle of Changes in rRNA transcription correlate with the development cycle.Active transcription periods for three different rRNA gene units are indicated.

Citation: Sharma I, McCutchan T. 2005. Ribosomes and Opportunities for Drug Intervention, p 353-364. In Sherman I (ed), Molecular Approaches to Malaria. ASM Press, Washington, DC. doi: 10.1128/9781555817558.ch18
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Image of FIGURE 2
FIGURE 2

Two-dimensional structure of the rRNA GTPase site, showing the location of sequence differences between the A-type and S-type RNAs.

Citation: Sharma I, McCutchan T. 2005. Ribosomes and Opportunities for Drug Intervention, p 353-364. In Sherman I (ed), Molecular Approaches to Malaria. ASM Press, Washington, DC. doi: 10.1128/9781555817558.ch18
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Image of FIGURE 3
FIGURE 3

Schematic presentation of the classical pseudoknot configuration.

Citation: Sharma I, McCutchan T. 2005. Ribosomes and Opportunities for Drug Intervention, p 353-364. In Sherman I (ed), Molecular Approaches to Malaria. ASM Press, Washington, DC. doi: 10.1128/9781555817558.ch18
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References

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Tables

Generic image for table
TABLE 1

Antibiotics interacting with 16S rRNA

Citation: Sharma I, McCutchan T. 2005. Ribosomes and Opportunities for Drug Intervention, p 353-364. In Sherman I (ed), Molecular Approaches to Malaria. ASM Press, Washington, DC. doi: 10.1128/9781555817558.ch18
Generic image for table
TABLE 2

Antibiotics interacting with 23S rRNA

Citation: Sharma I, McCutchan T. 2005. Ribosomes and Opportunities for Drug Intervention, p 353-364. In Sherman I (ed), Molecular Approaches to Malaria. ASM Press, Washington, DC. doi: 10.1128/9781555817558.ch18

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