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Chapter 5 : The Transcriptome of the Malaria Parasite

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Abstract:

This chapter explains how such data, when combined, can give a comprehensive overview of the transcriptome of the malaria parasite throughout its life cycle. These data are expected to lead to the identification of key -specific regulatory targets, a fundamental step for the development of new antimalarials. Gene expression throughout the cell cycle is usually regulated by complex interactions between DNA regulatory motifs and transcription factors that bind to these motifs, as well as regulation by distinct chromatin structures. Parasite isolates possess a high degree of diversity within their genes because of duplications, deletions, or recombination events that provide the parasite with a huge repertoire of antigenic determinants. Before the complete release of the genome sequence, several genomic efforts were accomplished to investigate the transcriptome of the malaria parasite. The first large-scale expression study of was accomplished with a DNA microarray platform constructed using a nuclease-generated genomic library. With the completion of the genome, two transcriptional analyses covering the entire genome were published. Both techniques allow effective and complete genome design. The 70-mer oligonucleotide array study was confined to a high-resolution analysis of the erythrocytic cell cycle. Gene expression in parasitic protozoa has shown itself to be unique when it comes to mechanisms of regulation. For instance, and other members of the Trypanosomatidae have polycistronic transcription with maturation of their mRNA by transplicing events.

Citation: Le Roch K, Winzeler E. 2005. The Transcriptome of the Malaria Parasite , p 68-84. In Sherman I (ed), Molecular Approaches to Malaria. ASM Press, Washington, DC. doi: 10.1128/9781555817558.ch5

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Gene Expression and Regulation
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Transcription Start Site
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Bacteria and Archaea
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DNA Synthesis
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Fatty Acid Biosynthesis
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