Chapter 2 : Tetracycline Resistance Due to Ribosomal Protection Proteins

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There have been 11 ribosomal protection genes identified which code for cytoplasmic proteins that protect the ribosomes from the action of tetracycline in vitro and in vivo and confer resistance to tetracycline, doxycycline, and minocycline to the host. The ribosomal protection proteins are thought to interact with the base of h34 protein, within the ribosome, causing an allosteric disruption of the primary tetracycline binding site(s). This releases the tetracycline molecules from the ribosome and allows the ribosome to return to its normal posttranslocational conformational state, which was altered by the binding of tetracycline. Glycylcyclines compete with tetracycline for ribosomal binding sites because they have either identical or overlapping sites on the ribosome and have the same mode of action as tetracycline. The differences in host range may be partially due to the type of element that each gene is associated with. This may be relevant because conjugative transposons, in general, have less host specificity than do plasmids and are able to be transferred to unrelated species and genera. The ribosomal protection genes are associated with plasmids, transposons, and conjugative transposons. Conjugative transfer appears to be influenced by the flanking sequences which correlate with the location of the Tn-Tn family inserted into the chromosome.

Citation: Roberts M. 2005. Tetracycline Resistance Due to Ribosomal Protection Proteins, p 19-28. In White D, Alekshun M, McDermott P (ed), Frontiers in Antimicrobial Resistance. ASM Press, Washington, DC. doi: 10.1128/9781555817572.ch2

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Generic image for table
Table 1

G+C content of ribosomal protection genes

Percentages are rounded off to the next whole number.

Citation: Roberts M. 2005. Tetracycline Resistance Due to Ribosomal Protection Proteins, p 19-28. In White D, Alekshun M, McDermott P (ed), Frontiers in Antimicrobial Resistance. ASM Press, Washington, DC. doi: 10.1128/9781555817572.ch2
Generic image for table
Table 2

Distribution of ribosomal protection genes

Data from references .

Citation: Roberts M. 2005. Tetracycline Resistance Due to Ribosomal Protection Proteins, p 19-28. In White D, Alekshun M, McDermott P (ed), Frontiers in Antimicrobial Resistance. ASM Press, Washington, DC. doi: 10.1128/9781555817572.ch2

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