Chapter 20 : Pathogen Gene Expression during Intestinal Infection

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This chapter discusses what is known about bacterial gene expression during infection of the intestinal tract in animal models of disease. Despite the publication of numerous studies investigating gene expression by pathogens that colonize the intestinal tract, many of these studies were performed in vitro or in cell culture models of infection. While the importance of these investigations cannot be disputed, these systems do not fully represent the complex milieu of the intestinal tract, on which the chapter is focused. In addition, since many intestinal pathogens have the ability to cause serious systemic disease, bacterial gene expression has been examined at sites of systemic infection. DNA microarrays have been recently used to examine gene expression in rabbit ligated ileal loops. Genes encoding transcriptional regulatory factors, including , were absent from the set of serovar Typhimurium genes induced specifically in the small intestine. Of the three species that infect humans, and are enteric pathogens. harbors a virulence plasmid, known as pYV, that carries many genes required for virulence. Using a strain lacking this plasmid, chromosomal intestinal genes were identified in the PP of mice 24 h after oral infection. The examination of gene expression in diverse bacterial pathogens during intestinal infection has revealed much about the conditions experienced during this process.

Citation: Butler S, Tischler A, Camilli A. 2005. Pathogen Gene Expression during Intestinal Infection, p 283-299. In Nataro J, Cohen P, Mobley H, Weiser J (ed), Colonization of Mucosal Surfaces. ASM Press, Washington, DC. doi: 10.1128/9781555817619.ch20

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Toxin Coregulated Pilus
Type IV Pili
Peyer's Patches
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Table 1

Genes induced during intestinal infection by bacterial pathogens

Citation: Butler S, Tischler A, Camilli A. 2005. Pathogen Gene Expression during Intestinal Infection, p 283-299. In Nataro J, Cohen P, Mobley H, Weiser J (ed), Colonization of Mucosal Surfaces. ASM Press, Washington, DC. doi: 10.1128/9781555817619.ch20
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Table 2

Genes whose spatial and temporal expression patterns in the intestine have been studied

Citation: Butler S, Tischler A, Camilli A. 2005. Pathogen Gene Expression during Intestinal Infection, p 283-299. In Nataro J, Cohen P, Mobley H, Weiser J (ed), Colonization of Mucosal Surfaces. ASM Press, Washington, DC. doi: 10.1128/9781555817619.ch20
Generic image for table
Table 3

In vivo regulators of and genes identified using RIVET

Citation: Butler S, Tischler A, Camilli A. 2005. Pathogen Gene Expression during Intestinal Infection, p 283-299. In Nataro J, Cohen P, Mobley H, Weiser J (ed), Colonization of Mucosal Surfaces. ASM Press, Washington, DC. doi: 10.1128/9781555817619.ch20

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