Clinical Mycobacteriology (Tuberculosis) Laboratory: Services and Methods

Leonid Heifets; Edward Desmond

doi:10.1128/9781555817657.ch4

Chapter 4 : Clinical Mycobacteriology (Tuberculosis) Laboratory: Services and Methods

Authors: Leonid Heifets, Edward Desmond

Content Type: Monograph

Publication Year: 2005

Category: Bacterial Pathogenesis; Clinical Microbiology

Book DOI: 10.1128/9781555817657

Chapter DOI: 10.1128/9781555817657.ch4

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Abstract:

This chapter presents a broad picture of activities in a diagnostic mycobacteriology laboratory with a focus on Mycobacterium tuberculosis detection, identification, and drug susceptibility testing. Development of rapid methods for mycobacterial species identification and drug susceptibility testing became especially important because of the extremely rapid progression of tuberculosis in human immunodeficiency virus (HIV)-positive individuals and because of the growing rates of drug resistance. In the modern worldwide systems of information networks and effective transportation systems, laboratory service does not have to be in close proximity to the patient or physician. Quality assurance (QA) is a system targeting continuous improvement of quality of laboratory services. Three chromatographic methods have been proposed for the determination of mycobacterial species on the basis of their cell wall lipids: gas-liquid chromatography, high-pressure liquid chromatography (HPLC), and thin-layer chromatography. Of these approaches, HPLC is now the most popular in clinical mycobacteriology. The major problem was that HPLC, which is based on UV spectrophotometry to separate the ester peaks, required a large harvest of bacteria. Examples of some phenotypic methods which require bacterial growth but allow detection more rapidly by means other than conventional growth inhibition are described in the chapter. The future of tuberculosis laboratories and their ability to play a significant role in National Tuberculosis Programs in countries with a high burden of tuberculosis will depend on their ability to detect drug-resistant bacteria in a timely manner while taking financial constraints into account.

Citation: Heifets L, Desmond E. 2005. Clinical Mycobacteriology (Tuberculosis) Laboratory: Services and Methods, p 49-68. In Cole S, Eisenach K, McMurray D, Jacobs, Jr. W (ed), Tuberculosis and the Tubercle Bacillus. ASM Press, Washington, DC. doi: 10.1128/9781555817657.ch4

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References

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1. Albert, H.,, A. Heydenrych,, R. Mole,, A. Trollip,, and L. Blumberg. 2001. Evaluation of FASTPlaque TB-RIFTM, a rapid, manual test for the determination of rifampicin resistance from Mycobacterium tuberculosis cultures . Int. J. Tuberc. Lung Dis. 5: 906– 911.

2. Albert, H.,, A. P. Trollip,, R. J. Mole,, J. B. Hatch,, and L. Blumberg. 2002. Rapid indication of multidrug-resistant tuberculosis from liquid cultures using FASTPlaque TB-RIFTM, a manual phage-based test. Int. J. Tuberc. Lung Dis. 6: 523– 528.

3.American Thoracic Society. 1974. Policy statement: quality of the laboratory for mycobacterial disease. Am. Rev. Respir. Dis. 110: 376– 377.

4.American Thoracic Society. 1983. Levels of laboratory services . Am. Rev. Respir. Dis. 128: 213– 215.

5. Arnold, L. J.,, P. W. Hammond,, W. A. Wiese,, and N. C. Nelson. 1989. Assay formats involving acridinium-esterlabeled DNA probes. Clin. Chem. 35: 1588– 1594.

6.Association of State and Territorial Public Health Directors and U.S. Department of Health and Human Services. 1995. Mycobacterium tuberculosis: Assessing Your Laboratory. Centers for Disease Control and Prevention, Atlanta, Ga..

7. Bailey, G. V. J.,, D. Savic,, G. D. Gothi,, V. B. Naidu,, and S. S. Nair. 1967. Potential yield of pulmonary tuberculosis cases by direct microscopy of an acid-fast bacilli. Rev. Infect. Dis. 6: 214– 222.

8. Banaiee, N.,, S. Bobadilla-de-Valle,, S. Bardarov, Jr.,, P. Riska,, P. M. Small,, A. Ponce-de-Leon,, W. R. Jacobs, Jr.,, G. F. Hatfull,, and J. Sifuentes-Osornio. 2001. Luciferase reporter mycobacteriophages for detection, identification and antibiotic susceptibility testing of Mycobacterium tuberculosis in Mexico. J. Clin. Microbiol. 39: 3883– 3888.

9. Banfi, E.,, G. Scialino,, and C. Monti-Bragadin. 2003. Development of a microdilution method to evaluate mycobacterium tuberculosis drug susceptibility. J. Antimicrob. Chemother. 5: 796– 800.

10. Bardarov, S., Jr.,, H. Dou,, K. D. Eisenach,, N. Banaiee,, S. Ya,, J. Chan,, W. R. Jacobs Jr., , and P. F. Riska. 2003. Detection and drug-susceptibility testing of M. tuberculosis from sputum samples using luciferase reporter phage: comparison with the Mycobacteria Growth Indicator Tube (MGIT) system. Diagn. Microbiol. Infect. Dis. 45: 53– 61.

11. Bemer, P.,, F. Palicova,, S. Rusch-Gerdes,, H. Drugeon,, and G. Pfyffer. 2002. Multicenter evaluation of fully automated BACTEC Mycobacteria Growth Indicator Tube 960 system for susceptibility testing of Mycobacterium tuberculosis. J. Clin. Microbiol. 40: 150– 154.

12. Bönicke, R. 1958. Die differenzierung humaner and boviner tuberkelterien mit hilfe von thiophen-2-carbonsaure-hydrazid. Naturwissenschaften 46: 392– 393.

13. Butler, W. R.,, and J. O. Kilburn. 1988. Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids. J. Clin. Microbiol. 26: 50– 53.

14. Butler, W. R.,, K. C. Jost,, and J. O. Kilburn. 1991. Identification of mycobacteria by high-performance liquid chromatography. J. Clin. Microbiol. 29: 2468– 2472.

15. Butler, W. R.,, and L. S. Guthertz. 2001. Mycolic acid analysis by high-performance liquid chromatography for identification of Mycobacterium species . Clin. Microbiol. Rev. 14: 704– 726.

16. Canetti, G.,, W. Fox,, A. Khomenko,, H. T. Mahler,, M. K. Menon,, D. A. Mitchison,, M. Rist,, and M. A. Smelev. 1969. Advances in techniques of testing mycobacterial drug sensitivity and the use of sensitivity tests in tuberculosis control programs. Bull. W. H. O. 41: 21– 43.

17. Canetti, G.,, S. Froman,, J. Grosset,, P. Hauduroy,, M. Langerova,, H. T. Mahler,, G. Meissner,, D. A. Mitchison,, and L. Sula. 1963. Mycobacteria: laboratory methods for testing drug sensitivity and resistance. Bull. W. H. O. 29: 565– 578.

18. Carriere, C.,, P. F. Riska,, O. Zimhony,, J. Kriakov,, S. Bardarov,, J. Burns,, J. Chan,, and W. R. Jacobs, Jr. 1997 Conditionally replicating luciferase reporter phages: improves sensitivity for rapid detection and assessment of drug susceptibility of mycobacterium tuberculosis. J. Clin. Microbiol. 35: 3232– 3239.

19. Caviedes, L.,, J. Delgado,, and R. H. Gilman. 2002. Tetrazolium microplate assay as a rapid and inexpensive colorimetric method for determination of antibiotic susceptibility of Mycobacterium tuberculosis. J. Clin. Microbiol. 40: 1873– 1874.

20. Caviedes, L.,, T. Lee,, R. H. Gilman,, P. Sheen,, E. Spellman,, E. H. Lee,, D. E. Berg,, S. Montenegro-James, and the Tuberculosis Group in Peru. 2000. Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. J. Clin. Microbiol. 38: 1203– 1208.

21. Cohn, M. L.,, R. F. Waggoner,, and J. K. McClatchy. 1968. The 7H11 medium for the culture of mycobacteria. Am. Rev. Respir. Dis. 98: 295– 296.

22. Desmond, E.,, and K. Loretz. 2001. Use of the Gen-Probe amplified Mycobacterium Tuberculosis Direct Test for early de- tection of Mycobacterium tuberculosis in BACTEC 12B medium. J. Clin. Microbiol. 39: 1993– 1995.

23. Dixon, D.,, and E. H. Gutherberg. 1967. Isolation of tubercle bacilli from uncentrifuged sputum on pyruvic acid medium. Am. Rev. Respir. Dis. 96: 119– 122.

24. Eisenach, K. D.,, M. D. Sifford,, M. D. Cave,, J. H. Bates,, and J. T. Crawford. 1991. Detection of Mycobacterium tuberculosis in sputum samples using polymerase chain reaction . Am. Rev. Respir. Dis. 144: 1160– 1163.

25. Enarson, D. A. 1995. The International Union Against Tuberculosis and Lung Disease. Model national tuberculosis programmes. Tubercle Lung Dis. 76: 95– 99.

26. Fleming, D. O.,, J. H. Richardson,, J. J. Tulis,, and D. Vesley (ed.). 1995. Laboratory Safety, 2nd ed. ASM Press, Washington, D.C..

27. Forbes, B. A.,, and K. E. S. Hicks. 1993. Direct detection of Mycobacterium tuberculosis in respiratory specimens in a clinical laboratory by polymerase chain reaction. J. Clin. Microbiol. 31: 1688– 1694.

28. Foulds, J.,, and R. O’Brien. 1998. New tools of the diagnosis of tuberculosis: the perspective for developing countries. Int. J. Tuberc. Lung Dis. 3: 1– 18.

29. Franzblau, S. G.,, R. S. Gitzig,, J. C. McLaughlin,, P. Torres,, G. Madico,, A. Hernandez,, M. T. Degnan,, M. B. Cook,, V. K. Quenzer,, R. M. Ferguson,, and R. H Gilman. 1998. Rapid, Low-technology MIC determination with clinical Mycobacterium tuberculosis isolates by using the microplate Alamar Blue assay. J. Clin. Microbiol. 36: 362– 366.

30. Glickman, S. E.,, S. O. Kilburn,, and W. R. Butler. 1994. Rapid identification of mycolic acid patterns of mycobacteria by high-performance liquid chromatography using pattern recognition software and a Mycobacterium library. J. Clin. Microbiol. 32: 740– 749.

31. Griffith, D. E.,, H. L. Barrett,, H. L. Bodily,, and R. M. Wood. 1967. Drug susceptibility tests for tuberculosis using drug impregnated discs. Am. J. Clin. Pathol. 47: 812– 817.

32. Griffith, M.,, and H. Bodily. 1992. Stability of antimycobacterial drugs in susceptibility testing. Antimicrob. Agents Chemother. 36: 2398– 2402.

33. Guthertz, L. S.,, S. D. Lim,, Y. Jang,, and P. S. Duffey. 1993. Curvilinear-gradient high-performance liquid chromatography for identification of mycobacteria. J. Clin. Microbiol. 31: 1876-– 881.

34. Hall, G. S. 1996. Primary processing of specimens, isolation and cultivation of mycobacteria. Clin. Lab. Med. 16: 551– 558.

35. Harrington, J. M.,, and H. S. Shannon. 1976. Incidence of tuberculosis, hepatitis, brucellosis, and shigellosis in British medical laboratory workers. Br. Med. J. 1: 759– 762.

36. Hazbon, M. H.,, N. Guarin,, B. E. Ferro,, A. L. Rodriguez,, L. A. Labrada,, R. Tovar,, P. F. Riska,, and W. R. Jacobs, Jr. 2003. Photographic and luminometric detection of luciferase reporter phages for drug susceptibility testing of clinical Mycobacterium tuberculosis isolates. J. Clin. Microbiol. 41: 4865– 4869.

37. Heifets, L. 1986. Rapid automated method (BACTEC system) in clinical mycobacteriology . Semin. Respir. Med. 1: 242– 249.

38. Heifets, L. 2001. False diagnosis of tuberculosis . Int. J. Tuberc. Lung Dis. 5: 789– 790.

39. Heifets, L.,, and G. A. Cangelosi. 1999. Drug susceptibility testing of Mycobacterium tuberculosis—a neglected problem at the turn of the century. Int. J. Tuberc. Lung Dis. 3: 1– 18.

40. Heifets, L.,, and P. A. Jenkins,. 1998. Speciation of mycobacteria in clinical laboratories, p. 308– 350. In P. R. Gangadharam, and P. Jenkins (ed.), Mycobacteria, I. Basic Aspects. Chapman and Hall, Thompson Science, New York, NY..

41. Heifets, L. 1996. Drug susceptibility testing in mycobacteriology. Clin. Lab. Med. 16: 641– 656.

42. Heifets, L., 1991. Drug susceptibility tests in the management of chemotherapy of tuberculosis, p. 89– 121. In L. B. Heifets (ed.), Drug Susceptibility in the Chemotherapy of Mycobacterial Infections. CRC Press, Inc., Boca Raton, Fl..

43. Heifets, L.,, T. Lindler,, T. Sanchez,, D. Spencer,, and J. Brennan. 2000. Two liquid medium systems, Mycobacterial Growth Indicator Tube and MB Redox Tube, for Mycobacterium tuberculosis isolation from sputum specimens. J. Clin. Microbiol. 38: 1227– 1230.

44. Heifets, L.,, T. Sanchez,, J. Vanderkolk,, and V. Pham. 1999. Development of rifapentine susceptibility tests for Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 43: 25– 28.

45. Heifets, L. B., 1999. Pyrazinamide, p. 668– 676. In V. Yu,, T. C. Merigan,, and S. L. Barriere (ed.), Antimicrobial Therapy and Vaccines. The Williams & Wilkins Co., Baltimore, Md..

46. Hoffner, S. E.,, M. Haile,, and G. Kallenius. 1992. A biphasic system for primary isolation of mycobactia compared to solid medium and broth culture. J. Med. Microbiol. 37: 332– 334.

47. Hultmann, T.,, S. Stahl,, and E. Hornes. 1980. Direct solid phase sequencing of genomic and plasmlid DNA using magnetic beads as support. Nucleic Acids Res. 17: 4939– 4946.

48. Isenberg, H. D. (ed.). 1992. Mycobacteriology, 3.1.1– 3.16.4. In Clinical Microbiology Procedure Handbook. ASM Press, Washington, D.C..

49. Isenberg, H. D.,, R. F. D’Amato,, L. Heifets,, P. R. Murray,, M. Scardamaglia,, M. C. Jacobs,, P. Alperstein,, and A. Niles. 1991. Collaborative feasibility study of a biphasic system (Roche Septi-Chek AFB) for rapid detection and isolation of mycobacteria. J. Clin. Microbiol. 29: 1719– 22.

50. Jonas, V.,, M. J. Alden,, J. I. Curri,, K. Kamisango,, C. A. Knott,, R. Lankford,, J. M. Wolfe,, and D. F. Moore. 1993. Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplification of rRNA. J. Clin. Microbiol. 31: 2410– 2416.

51. Jost, K. C., Jr.,, D. F. Dunbar,, S. S. Barth,, V. L. Headley,, and L. B. Elliot. 1995. Identification of Mycobacterium tuberculosis and M. avium complex directly from smear-positive sputum specimens and BACTEC 12B cultures by highperformance liquid chromatography with fluorescence detection and computer-driven pattern recognition models. J. Clin. Microbiol. 33: 1270– 1277.

52. Kent, P. T.,, and G. P. Kubica. 1985. Public Health Mycobacteriology: a Guide for the Level III Laboratory. Centers for Disease Control and Prevention, Atlanta, Ga..

53. Kilburn, J. O.,, and G. P. Kubica. 1968. Reagent impregnated paper strips for detection of niacin. Am. J. Clin. Pathol. 50: 530– 532.

54. Kirschner, P.,, A. Meier,, and E. C. Böttger,. 1993. Genotypic identification and detection of mycobacteria facing novel and uncultured pathogens, p. 173– 190. In D. H. Persing,, T. F. Smith,, F. C. Tenover,, and T. J. White (ed.), Diagnostic Molecular Microbiology. American Society for Microbiology, Washington, D.C..

55. Kirschner, P.,, B. Springer,, and U. Vogel. 1993. Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a two year experience in a clinical laboratory. J. Clin. Microbiol. 31: 2882– 2889.

56. Kisa, O.,, A. Albay,, O. Bedir,, O. Baylan,, and L. Doganci. 2003. Evaluation of FASTPlaqueTB-RIFTM for determination of rifampicin resistance in Mycobacterium tuberculosis complex isolates. Int. J. Tuberc. Lung Dis. 7: 284– 288.

57. Kleeberg, H. H. 1985. A simple method for testing the drug susceptibility of M. tuberculosis, a report of an international collaborative study. Bull. Int. Union Tuberc. 60: 147– 153.

58. Konno, K. 1956. New chemical method to differentiate human- type tubercle bacilli from other mycobacteria. Science 124: 985.

59. Kubica, G. P. 1964. A combined niacin-nitrate reduction test for use in the identification of mycobacteria. Acta Tuberc. Scand. 45: 161– 167.

60. Kubica, G. P.,, W. E. Dye,, M. L. Cohn,, and G. Middlebrook. 1963. Sputum digestion and decontamination with N-acetyl- L-cysteine-sodium hydroxide for culture of mycobacteria. Am. Rev. Respir. Dis. 87: 775– 779.

61. Kubica, G. P.,, W. M. Gross,, J. E. Hawkins,, A. Sommers,, L. Vestal,, and L. G. Wayne. 1975. Laboratory services for mycobacterial diseases. Am. Rev. Respir. Dis. 112: 773– 787.

62. Kubica, G. P.,, A. J. Kauffmann,, and W. E. Dye. 1964. Comments on the use of the new mucolytic agent N-acetyl-L-cysteine as a sputum digestant for the isolation of mycobacteria. Am. Rev. Respir. Dis. 89: 284– 286.

63. Laszlo, A. 1996. Tuberculosis bacteriology laboratory services and incremental protocols for developing countries. Clin. Lab. Med. 16: 697– 716.

64. McClatchy, J. K.,, R. F. Waggoner,, W. Kanes,, M. S. Cernich,, and T. L. Bolton. 1976. Isolation of mycobacteria from clinical specimens by use of selective 7H11 medium. Am. J. Clin. Pathol. 65: 412– 415.

65. Middlebrook, G.,, and M. L. Cohn. 1958. Bacteriology of tuberculosis: laboratory methods . Am. J. Public Health 48: 844– 853.

66. Middlebrook, G.,, L. Cohn,, W. E. Dye,, W. F. Russell,, and D. Levy. 1960. Microbiologic procedures of value in tuberculosis. Acta Tuberc. Scand. 38: 66– 81.

67. Middlebrook, G.,, Z. Reggiardo,, and W. D. Tigertt. 1977. Automatable radiometric detection of growth of M. tuberculosis in selective media. Am. Rev. Respir. Dis. 115: 1066– 1069.

68. Nolte, F. S.,, and B. Metchock,. 1995. Mycobacterium, p. 400– 449. In P. R. Murray,, E. J. Baron,, M. A. Pfaller,, F. C. Tenover,, and R. H. Yolken (ed.), Manual of Clinical Microbiology. ASM Press, Washington, D.C..

69. Palomono, J. C., 2000. Novel rapid antimicrobial susceptibility tests for M. tuberculosis, p. 145– 162. In I. Bastian, and F. Portaels (ed.), Multi-drug Resistant Tuberculosis, Kluwer Academic Publishing, Dordrecht, The Netherlands.

70. Palomino, J. C.,, A. Martin,, H. Camacho,, J. Guerra,, J. Swings,, and F. Portaels. 2002. Resazurin microtiter assay plate: simple and inexpensive methods for detection of drug resistance in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 46: 2720– 2722.

71. Palomino, J. C.,, and F. Portaels. 1999. Simple procedure for drug susceptibility testing of Mycobacterium tuberculosis using a commercial colorimetic assay. Eur. J. Clin. Microbiol. Infect. Dis. 18: 380– 383.

72. Park, W. G.,, W. R. Bishai,, R. E. Chaisson,, and S. E. Dorman. 2002. Performance of the microscopic observation drug susceptibility assay in drug susceptibility testing for Mycobacterium tuberculosis. J. Clin. Microbiol. 40: 4750– 4752.

73. Pfyffer, G. E.,, P. Kissling,, R. Wirth,, and R. Weber. 1994. Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by a target-amplified test system. J. Clin. Microbiol. 32: 918– 923.

74. Pike, R. M. 1976. Laboratory-associated infections: summary and analysis of 3,921 cases. Health Lab. Sci. 13: 105– 114.

75. Pike, R. M.,, S. E. Sulkin,, and M. L. Schulze. 1965. Continuing importance of laboratory-acquired infections. Am. J. Public Health 55: 190– 199.

76. Quigley, H. S., and H. R. Elston. 1970. Nitrite strips for detection of nitrite reduction by mycobacteria. Am. J. Clin. Pathol. 53: 663– 665.

77. Reid, D. D. 1957. Incidence of tuberculosis among workers in medical laboratories . Br. Med. J. 2: 10– 14.

78. Richmond, J. Y.,, and R. W. Mckinney (ed.). 1993. Biosafety in Microbiological and Biomedical Laboratories, 3rd ed. Publication 93-8395. Centers for Disease Control, Atlanta, Ga..

79. Richmond, J. Y.,, R. C. Knudsen,, and R. C. Good. 1996. Biosafety in the clinical mycobacteriology laboratory. Clin. Lab. Med. 6: 527– 550.

80. Riska, P. F.,, and W. R. Jacobs, Jr. 1998. The use of luciferasereporter phage for antibiotic-susceptibility testing of mycobacteria. Methods Mol. Biol. 101: 431– 453.

81. Riska, P. F.,, W. R. Jacobs, Jr.,, B. R. Bloom,, J. McKitrick,, and J. Chan. 1997. Specific identification of Mycobacterium tuberculosis with the luciferase reporter mycobacteriophage: use of p-nitro-α-acetylamino-β-hydroxy propiophenone. J. Clin. Microbiol. 35: 3225– 3231.

82. Riska, P. F.,, Y. Su,, S. Bardarov,, L. Freundlich,, G. Sarkie,, G. Hatfull,, C. Carriere,, V. Kumar,, J. Chan,, and W. R. Jacobs, Jr. 1999. Rapid film-based determination of antibiotic susceptibilities of Mycobacterium tuberculosis strains by using a luciferase reporter phage and the Bronx Box. J. Clin. Microbiol. 37: 1144– 1149.

83. Roberts, G.,, N. L. Goodman,, L. Heifets,, H. W. Larsh,, T. H. Lindner,, J. K. McClatchy,, M. R. McGinnis,, S. H. Siddiqi,, and P. Wright. 1983. Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear-positive specimens. J. Clin. Microbiol. 18: 689– 696.

84. Roberts, G. D.,, E. C. Böttger,, and L. Stockman. 1996. Rapid methods for the identification of mycobacterial species. Clin. Lab. Med. 16: 603– 616.

85. Roberts, G. D.,, E. W. Koneman,, and Y. K. Kim,. 1991. Mycobacterium, p. 304– 339. In A. Balows,, W. J. Hausles,, K. L. Herrmann,, H. D. Isenberg,, and H. J. Shadomy (ed.), Manual of Clinical Microbiology, 5th ed. American Society for Microbiology, Washington, D.C..

86. Rogall, T.,, T. Flohr,, and E. C. Böttger. 1990. Differentiation of Mycobacterium species by direct sequencing of amplified DNA. J. Gen. Microbiol. 136: 1915– 1920.

87. Sanchez, T.,, J. Vanderkolk,, S. Seay,, and L. Heifets. 1994. Quantitation of mycobacteria in blood specimens from patients with AIDS. Tubercle Lung Dis. 75: 386– 390.

88. Sewell, D. L.,, and R. B. Schifman,. 1995. Quality assurance: quality improvement, quality control, and test validation, p. 55– 85. In P. R. Murray,, E. J. Baron,, M. A. Pfaller,, F. C. Tenover,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 6th ed. ASM Press, Washington, D.C..

89. Shinnick, T. M.,, and V. Jonas,. 1994. Molecular approach to the diagnosis of tuberculosis, p. 517– 530. In B. R. Bloom, (ed.), Tuberculosis: Pathogenesis, Protection, and Control. ASM Press, Washington, D.C..

90. Siddiqi, S. H.,, J. P. Libonati,, and G. Middlebrook. 1981. Evaluation of rapid radiometric method for drug susceptibility testing of Mycobacterium tuberculosis. J. Clin. Microbiol. 13: 908– 912.

91. Sjobring, U.,, M. Mecklenburg,, A. B. Andersen,, and H. Miorner. 1990. Polymerase chain reaction for detection of Mycobacterium tuberculosis. J. Clin. Microbiol. 28: 2200– 2204.

92. Skinholj, P. 1974. Occupational risks in Danish clinical chemical laboratories. II. Infections. Scand. J. Clin. Lab. Investig. 33: 27– 29.

93. Smithwick, R. W. 1976. Laboratory Manual for Acid Fast Microscopy. Centers for Disease Control, Atlanta, Ga..

94. Sommers, H. M.,, and J. K. McClatchy,. 1983. Cumitech 16, Laboratory Diagnosis of the Mycobacterioses. Josephine A. Morello, Coordinating ed. American Society for Microbiology, Washington, D.C..

95. Spargo, C. A.,, P. D. Haaland,, S. R. Jurgensen,, D. D. Shank,, and G. T. Walker. 1993. Chemiluminescent detection of strand displacement amplified DNA from species comprising the Mycobacterium tuberculosis complex. Mol. Cell. Probes 7: 395– 404.

96. Sritharan, V.,, and R. H. Barker, Jr. 1991. A simple method for diagnosing M. tuberculosis infection in clinical samples using PCR. Mol. Cell. Probes 5: 385– 395.

97. Takiff, H. E., 2000. The molecular mechanisms of drug resistance in M. tuberculosis, p. 77– 114. In I. Bastian, and F. Portaels (ed.), Multi-Drug Resistant Tuberculosis. Kluwer Academic Publishing, Dordrecht, The Netherlands.

98. Telenti, A. 1997. Genetics of drug resistance in tuberculosis. Clin. Chest Med. 18: 55– 64.

99. Tenover, F. C.,, J. T. Crawford,, R. E. Heubner,, L. J. Geiter,, C. R. Horsburgh,, and R. C. Good. 1993. The resurgence of tuberculosis: is your laboratory ready? J. Clin. Microbiol. 31: 767– 770.

100. Thibert, L.,, and S. Lapierre. 1993. Routine application of high-performance liquid chromatography for identification of mycobacteria. J. Clin. Microbiol. 31: 1759– 1763.

101. Tortoli, E.,, F. Mandler,, and M. Bartolucci. 1993. Multicenter evaluation of a biphasic culture system for recovery of mycobacteria from clinical specimens. Eur. J. Clin. Microbiol. Infect. Dis. 12: 425– 429.

102. Vestal, A. L. 1977. Procedures for the Isolation and Identification of Mycobacteria. HEW publication (CDC) 77-8230. Centers for Disease Control, Atlanta, Ga..

103. Vestal, A. L.,, and G. P. Kubica. 1967. Differential identification of mycobacteria. III. Use of thiacetazone, thiophen-2- carboxylic acid hydrozide and triphenyltetrazolim chloride. Scand. J. Respir. Dis. 48: 142– 148.

104. Virtanen, S. 1960. A study of nitrate reduction by mycobacteria. Acta Tuberc. Scand. Suppl. 48: 111– 119.

105. Walker, G. T.,, M. C. Little,, J. G. Nadeau,, and D. D. Shank. 1992. Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. Proc. Natl. Acad. Sci. USA 89: 392– 396.

106. Warren, N. G.,, and J. R. Cord. 1996. Activities and recommendations by the Association of State and Territorial Public Health Laboratory Directors. Clin. Lab. Med. 16: 551– 568.

107. Wayne, L. G. 1974. Simple pyrazinamide and urease tests for routine identification of mycobacteria. Am. Rev. Respir. Dis. 109: 147– 151.

108. Wayne, L. G.,, and I. Krasnow. 1966. Preparation of tuberculosis susceptibility testing medium by means of impregnated discs. Am. J. Clin. Pathol. 45: 769– 771.

109. Welch, D. F.,, A. P. Guruswamy,, S. J. Sides,, C. H. Shaw,, and M. J. R. Gilchrist. 1993. Timely culture for mycobacteria which utilizes a microcolony method. J. Clin. Microbiol. 31: 2178– 2184.

110. Wilson, S. M.,, Z. al-Suwaidi,, R. McNerney,, J. Porter,, and F. Drobniewski. 1997. Evaluation of a new rapid bacteriophage- based method for the drug susceptibility testing of Mycobacterium tuberculosis. Nat. Med. 3: 465– 468.

111. Witebsky, F. G.,, and P. Kruczak-Filipov. 1996. Identification of mycobacteria by conventional methods. Clin. Lab. Med. 16: 569– 602.

112. Woods, G. L.,, and J. C. Ridderhof. 1996. Quality assurance in the mycobacteriology laboratory. Clin. Lab. Med. 16: 657– 675.

113. Woods, G. L.,, and J. A. Washington. 1987. Mycobacteria other than M. tuberculosis: a review of microbiologic and clinical aspects. Rev. Infect. Dis. 9: 275– 294.

114. Woods, G. L.,, and F. G. Witebsky. 1995. College of American Pathologists mycobacteriology E Proficiency Testing Survey. Summary of participant performance, 1979-1992. Arch. Pathol. Lab. Med. 119: 17– 22.

115. Young, W. D., Jr., A. Maslansky, M. S. Lefar, and D. P. Kronish. 1970. Development of a paper strip test for detection of niacin produced by mycobacteria. Appl. Microbiol. 20: 939– 945.

Tables

Clinical Mycobacteriology (Tuberculosis) Laboratory: Services and Methods

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Table 1

Differentiation among the members of the M. tuberculosis complex

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Table 1

Differentiation among the members of the M. tuberculosis complex

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Table 2

Critical concentrations for M. tuberculosis in different media

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Table 2

Critical concentrations for M. tuberculosis in different media