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Chapter 5 : Immune-Based Methods

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Abstract:

On infection with , antigen-specific humoral and cell-mediated immune responses are elicited in the host. This chapter reviews the recent advances toward the development of diagnostic tests for clinical and latent tuberculosis (TB) based on the identification of immunodominant antigens of and characterization of both the antibody and T-cell responses mounted by the host. The local in situ environment in the lung is different at these different stages; the bacterial loads vary, being significantly higher in individuals with cavitary lesions where the opportunity to replicate extracellularly exists. In recent years, several new antigens of have been cloned and evaluated for their serodiagnostic potential. The proteins present in culture filtrates of replicating in bacteriological media have been extensively evaluated based on the presumption that the same antigens would also be expressed by the in vivo replicating bacteria. The immune dysfunction caused by progressive human immunodeficiency virus (HIV) infection results in high rates of reactivation of latent TB, an increased incidence of progression of infection to primary TB, and an accelerated course of disease progression. Cellular immune responses elicited on infection with involve the generation of both CD4 and CD8 T-cell subsets.

Citation: Laal S, Skeiky Y. 2005. Immune-Based Methods, p 71-83. In Cole S, Eisenach K, McMurray D, Jacobs, Jr. W (ed), Tuberculosis and the Tubercle Bacillus. ASM Press, Washington, DC. doi: 10.1128/9781555817657.ch5

Key Concept Ranking

Major Histocompatibility Complex
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Cell-Mediated Immune Response
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Humoral Immune Response
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References

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1. Alderson, M. R.,, T. Bement,, C. H. Day,, L. Zhu,, D. Molesh,, Y. A. Skeiky,, R. Coler,, D. M. Lewinsohn,, S. G. Reed,, and D. C. Dillon. 2000. Expression cloning of an immunodominant family of Mycobacterium tuberculosis antigens using human CD4(+) T cells. J. Exp. Med. 191:551560.
2. Andersen, A. B.,, and E. B. Hansen. 1989. Structure and mapping of antigenic domains of protein antigen b, a 38,000- molecular-weight protein of Mycobacterium tuberculosis. Infect. Immun. 57:24812488.
3. Andersen, P.,, A. B. Andersen,, A. L. Sorensen,, and S. Nagai. 1995. Recall of long-lived immunity to Mycobacterium tuberculosis infection in mice. J. Imunnol. 154:33593372.
4. Ansari, N. A.,, A. H. Kombe,, T. A. Kenyon,, N. M. Hone,, J. W. Tappero,, S. T. Nyirenda,, N. J. Binkin,, and S. B. Lucas. 2002. Pathology and causes of death in a group of 128 predominantly HIV-positive patients in Botswana, 1997-1998. Int. J. Tuberc. Lung Dis. 6:5563.
5. Arend, S. M.,, P. Andersen,, K. E. van Meijgaarden,, R. L. Skjot,, Y. W. Subronto,, J. T. van Dissel,, and T. H. Ottenhoff. 2000. Detection of active tuberculosis infection by T cell responses to early-secreted antigenic target 6-kDa protein and culture filtrate protein 10. J. Infect. Dis. 181:18501854.
6. Arend, S. M.,, A. Geluk,, K. E. van Meijgaarden,, J. T. van Dissel,, M. Theisen,, P. Andersen,, and T. H. Ottenhoff. 2000. Antigenic equivalence of human T-cell responses to Mycobacterium tuberculosis-specific RD1-encoded protein antigens ESAT-6 and culture filtrate protein 10 and to mixtures of synthetic peptides. Infect. Immun. 68:33143321.
7. Behr, M. A.,, M. A. Wilson,, W. P. Gill,, H. Salamon,, G. K. Schoolnik,, S. Rane,, and P. M. Small. 1999. Comparative genomics of BCG vaccines by whole-genome DNA microarray. Science 284:15201523.
8. Binkin, N. J.,, A. A. Vernon,, P. M. Simone,, E. McCray,, B. I. Miller,, C. W. Schieffelbein,, and K. G. Castro. 1999. Tuberculosis prevention and control activities in the United States: an overview of the organization of tuberculosis services. Int. J. Tuberc. Lung. Dis. 3:663674.
9. Bothamley, G. H.,, R. Rudd,, F. Festenstein,, and J. Ivanyi. 1992. Clinical value of the measurement of Mycobacterium tuberculosis specific antibody in pulmonary tuberculosis. Thorax 47:270275.
10. Brock, I.,, M. E. Munk,, A. Kok-Jensen,, and P. Andersen. 2001. Performance of whole blood IFN-gamma test for tuberculosis diagnosis based on PPD or the specific antigens ESAT-6 and CFP-10. Int. J. Tuberc. Lung Dis. 5:462467.
11. Brusasca, P. N.,, R. Colangeli,, K. P. Lyashchenko,, X. Zhao,, M. Vogelstein,, J. S. Spencer,, D. N. McMurray,, and M. L. Gennaro. 2001. Immunological characterization of antigens encoded by the RD1 region of the Mycobacterium tuberculosis genome. Scand. J. Immunol. 54:448452.
12. Campos-Neto, A.,, V. Rodrigues-Junior,, D. B. Pedral-Sampaio,, E. M. Netto,, P. J. Ovendale,, R. N. Coler,, Y. A. Skeiky,, R. Badaro,, and S. G. Reed. 2001. Evaluation of DPPD, a single recombinant Mycobacterium tuberculosis protein as an alternative antigen for the Mantoux test. Tuberculosis (Edinburgh) 81:353358.
13. Colangeli, R.,, A. Antinori,, A. Cingolani,, L. Ortona,, K. Lyashchenko,, G. Fadda,, and M. L. Gennaro. 1999. Humoral immune responses to multiple antigens of Mycobacterium tuberculosis in tuberculosis patients co-infected with the human immunodeficiency virus. Int. J. Tuberc. Lung Dis. 3:11271131.
14. Colangeli, R.,, J. S. Spencer,, P. Bifani,, A. Williams,, K. Lyashchenko,, M. A. Keen,, P. J. Hill,, J. Belisle,, and M. L. Gennaro. 2000. MTSA-10, the product of the Rv3874 gene of Mycobacterium tuberculosis, elicits tuberculosis-specific, delayed-type hypersensitivity in guinea pigs. Infect. Immun. 68:990993.
15. Cole, S. T.,, R. Brosch,, J. Parkhill,, T. Garnier,, C. Churcher,, D. Harris,, S. V. Gordon,, K. Eiglmeier,, S. Gas,, C. E. Barry III,, F. Tekaia,, K. Badcock,, D. Basham,, D. Brown,, T. Chillingworth,, R. Connor,, R. Davies,, K. Devlin,, T. Feltwell,, S. Gentles,, N. Hamlin,, S. Holroyd,, T. Hornsby,, K. Jagels,, A. Krogh,, J. McLean,, S. Moule,, L. Murphy,, K. Oliver,, J. Osborne,, M. A. Quail,, M. A. Rajandream,, J. Rogers,, S. Rutter,, K. Seegar,, J. Skelton,, R. Squares,, S. squares,, J. E. Sulston,, K. Taylor,, S. Whitehead,, and B. G. Barell. 1998. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537544.
16. Coler, R. N.,, Y. A. Skeiky,, P. J. Ovendale,, T. S. Vedvick,, L. Gervassi,, J. Guderian,, S. Jen,, S. G. Reed,, and A. Campos- Neto. 2000. Cloning of a Mycobacterium tuberculosis gene encoding a purifed protein derivative protein that elicits strong tuberculosis-specific delayed-type hypersensitivity. J. Infect. Dis. 182:224233.
17. Coler, R. N.,, Y. A. W. Skeiky,, T. Vedvick,, T. Bement,, P. Ovendale,, A. Campos-Neto,, M. R. Alderson,, and S. G. Reed. 1998. Moleular cloning and Immunologic reactivity of a novel low molecular mass antigen of Mycobacterium tuberculosis. J. Immunol. 161:23562364.
18. Covert, B. A.,, J. S. Spencer,, I. M. Orme,, and J. T. Belisle. 2001. The application of proteomics in defining the T cell antigens of Mycobacterium tuberculosis. Proteomics 1:574586.
19. Daniel, T. M.,, and P. A. Anderson. 1978. The isolation by immunoabsorbent affinity chromatography and physiochemical characterization of Mycobacterium tuberculosis antigen. Am. Rev. Respir. Dis. 117:533539.
20. Daniel, T. M.,, and B. W. Janicki. 1978. Mycobacterial antigens: a review of their isolation, chemistry and immunological properties. Microbiol. Rev. 42:84113.
21. Daniel, T. M. 1996. Immunodiagnosis of tuberculosis, p. 223231. In S. Garay (ed.), Tuberculosis. Little, Brown & Co. Inc., Boston, Mass..
22. Dillon, D. C.,, M. R. Alderson,, C. H. Day,, T. Bement,, A. Campos- Neto,, Y. A. Skeiky,, T. Vedvick,, R. Badaro,, S. G. Reed,, and R. Houghton. 2000. Molecular and immunological characterization of Mycobacterium tuberculosis CFP-10, an immunodiagnostic antigen missing in Mycobacterium bovis BCG. J. Clin. Microbiol. 38:32853290.
23. Dillon, D. C.,, M. R. Alderson,, C. H. Day,, D. Lewinsohn,, R. Coler,, T. Bement,, A. Campos-Neto,, Y. A. W. Sheiky,, I. M. Orme,, A. Roberts,, S. Steen,, W. Dalemans,, R. Badaro,, and S. G. Reed. 1999. Molecular characterization and human T-cell responses to a member of a novel Mycobacterium tuberculosis mtb39 gene family. Infect. Immun. 67:29412950.
24. DiPerri, G.,, A. Cazzadori,, S. Vento,, M. Malena,, L. Bontempini,, M. Lanzafame,, B. Allegranzi,, and E. Concia. 1996. Comparative histopathological study of pulmonary tuberculosis in human immunodeficiency virus-infected and non-infected patients. Tubercle Lung Dis. 77:244249.
25. Doherty, T. M.,, A. Demissie,, J. Olobo,, D. Wolday,, S. Britton,, T. Eguale,, P. Ravn,, and P. Andersen. 2002. Immune responses to the Mycobacterium tuberculosis-specific antigen ESAT-6 signal subclinical infection among contacts of tuberculosis patients. J. Clin. Microbiol. 40:704706.
26. Elhay, M. J.,, T. Oettinger,, and P. Andersen. 1998. Delayed-type hypersensitivity responses to ESAT-6 and MPT64 from Mycobacterium tuberculosis in the guinea pig. Infect. Immun. 66:34543456.
27. Foulds, J.,, and R. O’Brien. 1998. New tools for the diagnosis of tuberculosis: the perspective of developing countries. Int. J. Tuberc. Lung Dis. 2:778783.
28. Harboe, M.,, T. Oettinger,, H. G. Wiker,, I. Rosenkrands,, and P. Andersen. 1996. Evidence for occurrence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG. Infect. Immun. 64:1622.
29. Harries, A. D.,, N. J. Hargreaves,, J. Kemp,, A. Jindani,, D. A. Enarson,, D. Maher,, and F. M. Salaniponi. 2001. Deaths from tuberculosis in sub-Saharan African countries with a high prevalence of HIV-1. Lancet 357:15191523.
30. Hawken, M. P., and D. W. Muhindi. 1999. Tuberculosis preventive therapy in HIV-infected persons: feasibility issues in developing countries. Int. J. Tuberc. Lung Dis. 3:646650.
31. Hendrickson, R. C.,, J. F. Douglass,, L. D. Reynolds,, P. D. Mc- Neill,, D. Carter,, S. G. Reed,, and R. L. Houghton. 2000. Mass spectrometric identification of Mtb81, a novel serological marker for tuberculosis. J. Clin. Microbiol. 38:23542361.
32. Hewitt, A. R. M. Coates, D. A. Mitchinson, and J. Ivanyi. 1982. The use of murine monoclonal antibodies without purification of antigen in the serodiagnosis of tuberculosis. J. Immunol. Methods 55:205211.
33. Hira, S. K.,, A. S. R. Srinivasa Rao,, and J. Thanekat. 1999. Evidence of AIDS-related mortality in Mumbai, India. Lancet 354:11751176.
34. Holmes, C. B.,, E. Losina,, R. P. Walensky,, Y. Yazdanpanah,, and K. A. Freedberg. 2003. Review of human immunodeficiency virus type 1-related opportunistic infections in sub- Saharan Africa. Clin. Infect. Dis. 36:652662.
35. Houghton, R. L.,, M. J. Lodes,, D. C. Dillon,, L. D. Reynolds,, C. H. Day,, P. D. McNeill,, R. C. Hendrickson,, Y. A. Skeiky,, D. P. Sampaio,, R. Badaro,, K. P. Lyashchenko,, and S. G. Reed. 2002. Use of multiepitope polyproteins in serodiagnosis of active tuberculosis. Clin. Diagn. Lab. Immunol. 9:883891.
36. Janoff, E. N.,, W. D. Hardy,, P. D. Smith,, and S. M. Wahl. 1991. Humoral recall responses in HIV infection. J. Immunol. 147:21302135.
37. Johnson, P. D.,, R. L. Stuart,, M. L. Grayson,, D. Olden,, A. Clancy,, P. Ravn,, P. Andersen,, W. J. Britton,, and J. S. Rothel. 1999. Tuberculin-purified protein derivative-, MPT-64-, and ESAT-6-stimulated gamma interferon responses in medical students before and after Mycobacterium bovis BCG vaccination and in patients with tuberculosis. Clin. Diagn. Lab. Immunol. 6:934937.
38. Jungblut, P. R.,, E. C. Muller,, J. Mattow,, and S. H. Kaufmann. 2001. Proteomics reveals open reading frames in Mycobacterium tuberculosis H37Rv not predicted by genomics. Infect. Immun. 69:59055907.
39. Jungblut, P. R.,, U. E. Schaible,, H. J. Mollenkopf,, U. Zimny- Arndt,, B. Raupach,, J. Mattow,, P. Halada,, S. Lamer,, K. Hagens,, and S. H. Kaufmann. 1999. Comparative proteome analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains: towards functional genomics of microbial pathogens. Mol. Microbiol. 33:11031117.
40. Kroon, F. P.,, J. T. Van Dissel,, J. Labadie,, A. M. Van Loon,, and R. Van Furth. 1995. Antibody response to diphtheria, tetanus, and poliomyelitis vaccines in relation to the number of cd4+ T lymphocytes in adults infected with human immunodeficiency virus. Clin. Infect. Dis. 21:11971203.
41. Laal, S.,, K. M. Samanich,, M. G. Sonnenberg,, J. T. Belisle,, J. O’Leary,, M. S. Simberkoff,, and S. Zolla-Pazner. 1997. Surrogate marker of preclinical tuberculosis in human immunodeficiency virus infection: antibodies to an 88 kDa secreted antigen of Mycobacterium tuberculosis. J. Infect. Dis. 176:133143.
42. Laal, S.,, K. M. Samanich,, M. G. Sonnenberg,, S. Zolla-Pazner,, J. M. Phadtare,, and J. T. Belisle. 1996. Human humoral responses to antigens of Mycobacterium tuberculosis: immunodominance of high molecular weight antigens. Clin. Diagn. Lab. Immunol. 4:4956.
43. Lein, A. D.,, C. F. von Reyn,, P. Ravn,, C. R. Horsburgh, Jr.,, L. N. Alexander,, and P. Andersen. 1999. Cellular immune responses to ESAT-6 discriminate between patients with pulmonary disease due to Mycobacterium avium complex and those with pulmonary disease due to Mycobacterium tuberculosis. Clin. Diagn. Lab. Immunol. 6:606609.
44. Lodes, M.,, D. C. Dillon,, R. L. Houghton,, and Y. A. Skeiky. 2003. Expression Cloning, 2nd ed. Humana Press, Totowa, N.J..
45. Lodes, M. J.,, D. C. Dillon,, R. Mohamath,, C. H. Day,, D. R. Benson,, L. D. Reynolds,, P. McNeill,, D. P. Sampaio,, Y. A. Skeiky,, R. Badaro,, D. H. Persing,, S. G. Reed,, and R. L. Houghton. 2001. Serological expression cloning and immunological evaluation of MTB48, a novel Mycobacterium tuberculosis antigen. J. Clin. Microbiol. 39:24852493.
46. Lyashchenko, K.,, R. Colangeli,, M. Houde,, H. Al Jahdali,, D. Menzies,, and M. Gennaro. 1998. Heterogeneous antibody responses in tuberculosis. Infect. Immun. 66:39363940.
47. Lyashchenko, K. P.,, M. Singh,, R. Colangeli,, and M. L. Gennaro. 2000. A multi-antigen print immunoassay for the development of serological diagnosis of infectious diseases. J. Immunol. Methods 242:91100.
48. MacVandiviere, H.,, W. E. Loring,, I. Melvin,, and S. Willis. 1956. The treated pulmonary lesion and its tubercle bacillus. II. The death and resurrection. Am. J. Med. Sci. 1956 (July): 3137.
49. Mahairas, G. G.,, P. J. Sabo,, M. J. Hickey,, D. C. Singh,, and C. K. Stover. 1996. Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis. J. Bacteriol. 178:12741282.
50. Martin-Casabona, N.,, T. G. Fuente,, F. Papa,, J. R. Urgell,, R. V. Pla,, G. C. Grau,, and I. R. Camps. 1992. Time course of anti- SL-IV immunoglobulin G antibodies in patients with tuberculosis and tuberculosis-associated AIDS. J. Clin. Microbiol. 30:10891093.
51. Mazurek, G. H.,, P. A. LoBue,, C. L. Daley,, J. Bernardo,, A. A. Lardizabal,, W. R. Bishai,, M. F. Iademarco,, and J. S. Rothel. 2001. Comparison of a whole-blood interferon gamma assay with tuberculin skin testing for detecting latent Mycobacterium tuberculosis infection. JAMA 286:17401747.
52. McKinney, J. D.,, K. Honer zu Bentrup,, E. J. Munoz-Elias,, A. Miczak,, B. Chen,, W. T. Chan,, D. Swenson,, J. C. Sacchettini,, W. R. Jacobs, Jr.,, and D. G. Russell. 2000. Persistence of Mycobacterium tuberculosis in macrophages and mice requires the glyoxylate shunt enzyme isocitrate lyase. Nature 406:735738.
53. Misra, S. N.,, D. Sengupta,, and S. K. Satpathy. 1998. AIDS in India: recent trends in opportunistic infections. Southeast Asian J. Trop. Med. Public Health 29:373376.
54. Mustafa, A. S.,, H. A. Amoudy,, H. G. Wiker,, A. T. Abal,, P. Ravn,, F. Oftung,, and P. Andersen. 1998. Comparison of antigen- specific T cell responses of tuberculosis patients using complex or single antigens of Mycobacterium tuberculosis. Scand. J. Immunol. 48:535543.
55. Mustafa, A. S.,, F. Oftung,, H. A. Amoudy,, N. M. Madi,, A. T. Abal,, F. Shaban,, I. Rosenkrands,, and P. Andersen. 2000. Multiple epitopes from the Mycobacterium tuberculosis ESAT-6 antigen are recognized by antigen-specific human T cell lines. Clin. Infect. Dis. 30(Suppl. 3):S201S205.
56. Nagai, S.,, H. G. Wiker,, M. Harboe,, and M. Kinomoto. 1991. Isolation and partial characterization of major protein antigens in the culture fluid of Mycobacterium tuberculosis. Infect. Immun. 59:372382.
57. Pethe, K.,, S. Alonso,, F. Biet,, G. Delogu,, M. J. Brennan,, C. Locht,, and F. D. Menozzi. 2001. The heparin-binding haemagglutinin of M. tuberculosis is required for extrapulmonary dissemination. Nature 412:190194.
58. Pottumarthy, S.,, V. C. Weels,, and A. J. Morris. 2000. A comparison of seven tests for serological diagnosis of tuberculosis. J. Clin. Microbiol. 38:22272231.
59. Roche, P. W.,, N. Winter,, J. A. Triccas,, C. G. Feng,, and W. J. Britton. 1996. Expression of Mycobacterium tuberculosis MPT64 in recombinant Mycobacterium smegmatis: purification, immunogenicity and application of skin tests for tuberculosis. Clin. Exp. Immunol. 103:226232.
60. Romain, F.,, C. Horn,, P. Pescher,, A. Namane,, M. Riviere,, G. Puzo,, O. Barzu,, and G. Marchal. 1999. Deglycosylation of the 45/47-kilodalton antigen complex of Mycobacterium tuberculosis decreases its capacity to elicit in vivo or in vitro cellular immune responses. Infect. Immun. 67:55675572.
61. Rosenkrands, I.,, K. Weldingh,, S. Jacobsen,, C. V. Hansen,, W. Florio,, I. Gianetri,, and P. Andersen. 2000. Mapping and identification of Mycobacterium tuberculosis proteins by twodimensional gel electrophoresis, microsequencing and immunodetection. Electrophoresis 21:935948.
62. Sada, E.,, L. E. Ferguson,, and T. M. Daniel. 1990. An ELISA for the serodiagnosis of tuberculosis using a 30,000-Da native antigen of Mycobacterium tuberculosis. J. Infect. Dis. 162:928931.
63. Samanich, K.,, J. T. Belisle,, and S. Laal. 2001. Homogeneity of antibody responses in tuberculosis patients. Infect. Immun. 69:46004609.
64. Samanich, K. M.,, J. T. Belisle,, M. G. Sonnenberg,, M. A. Keen,, S. Zolla-Pazner,, and S. Laal. 1998. Delineation of human antibody responses to culture filtrate antigens of Mycobacterium tuberculosis. J. Infect. Dis. 178:15341538.
65. Samanich, K. M.,, M. A. Keen,, V. D. Vissa,, J. D. Harder,, J. S. Spencer,, J. T. Belisle,, S. Zolla-Pazner,, and S. Laal. 2000. Serodiagnostic potential of culture filtrate antigens of Mycobacterium tuberculosis. Clin. Diagn. Lab. Immunol. 7:662668.
66. Skeiky, Y. A.,, M. J. Lodes,, J. A. Guderian,, R. Mohamath,, T. Bement,, M. R. Alderson,, and S. G. Reed. 1999. Cloning, expression, and immunological evaluation of two putative secreted serine protease antigens of Mycobacterium tuberculosis. Infect. Immun. 67:39984007.
67. Skeiky, Y. A.,, P. J. Ovendale,, S. Jen,, M. R. Alderson,, D. C. Dillon,, S. Smith,, C. B. Wilson,, I. M. Orme,, S. G. Reed,, and A. Campos-Neto. 2000. T cell expression cloning of a Mycobacterium tuberculosis gene encoding a protective antigen associated with the early control of infection. J. Immunol. 165: 71407149.
68. Skjot, R. L.,, I. Brock,, S. M. Arend,, M. E. Munk,, M. Theisen,, T. H. Ottenhoff,, and P. Andersen. 2002. Epitope mapping of the immunodominant antigen TB10.4 and the two homologous proteins TB10.3 and TB12.9, which constitute a subfamily of the esat-6 gene family. Infect. Immun. 70:54465453.
69. Skjot, R. L.,, T. Oettinger,, I. Rosenkrands,, P. Ravn,, I. Brock,, S. Jacobsen,, and P. Andersen. 2000. Comparative evaluation of low-molecular-mass proteins from Mycobacterium tuberculosis identifies members of the ESAT-6 family as immunodominant T-cell antigens. Infect. Immun. 68:214220.
70. Smith, H. 1998. What happens to bacterial pathogens in vivo? Trends Microbiol. 6:239243.
71. Sonnenberg, M. G.,, and J. T. Belisle. 1997. Definition of Mycobacterium tuberculosis culture filtrate proteins by twodimensional polyacrylamide gel electrophoresis, N-terminal amino acid sequencing and electrospray mass spectrometry. Infect. Immun. 65:45154524.
72. Streeton, J. A.,, N. Desem,, and S. L. Jones. 1998. Sensitivity and specificity of a gamma interferon blood test for tuberculosis infection. Int. J. Tuberc. Lung Dis. 2:443450.
73. Thybo, S.,, C. Richter,, H. Wachmann,, S. Y. Maselle,, D. H. Mwakyusa,, I. Mtoni,, and A. B. Anderson. 1995. Humoral response to Mycobacterium tuberculosis-specific antigens in African tuberculosis patients with high prevalence of human immunodeficiency virus infection. Tubercle Lung Dis. 76: 149155.
74. Urquhart, B. L.,, T. E. Atsalos,, D. Roach,, D. J. Basseal,, B. Bjellqvist,, W. L. Britton,, and I. Humphery-Smith. 1997. ‘Proteomic contigs’ of Mycobacterium tuberculosis and Mycobacterium bovis (BCG) using novel immobilised pH gradients. Electrophoresis 18:13841392.
75. van Pinxteren, L. A.,, P. Ravn,, E. M. Agger,, J. Pollock,, and P. Andersen. 2000. Diagnosis of tuberculosis based on the two specific antigens ESAT-6 and CFP10. Clin. Diagn. Lab. Immunol. 7:155160.
76. Vekemans, J.,, C. Lienhardt,, J. S. Sillah,, J. G. Wheeler,, G. P. Lahai,, M. T. Doherty,, T. Corrah,, P. Andersen,, K. P. McAdam,, and A. Marchant. 2001. Tuberculosis contacts but not patients have higher gamma interferon responses to ESAT-6 than do community controls in The Gambia. Infect. Immun. 69: 65546557.
77. Webb, J. R.,, T. S. Vedvick,, M. R. Alderson,, J. A. Guderian,, S. S. Jen,, P. J. Ovendale,, S. M. Johnson,, S. G. Reed,, and Y. A. Skeiky. 1998. Molecular cloning, expression, and immunogenicity of MTB12, a novel low-molecular-weight antigen secreted by Mycobacterium tuberculosis. Infect. Immun. 66:42084214.
78. Whalen, C.,, C. R. Horsburg,, D. Hom,, C. Lahart,, M. Simberkoff,, and J. Ellner. 1995. Accelerated course of human immunodeficiency virus infection after tuberculosis. Am. J. Respir. Crit. Care Med. 151:129135.
79. Wilcke, J.,, B. Jensen,, P. Ravn,, A. Andersen,, and K. Haslov. 1996. Clinical evaluation of MPT-64 and MPT-59, two protiens secreted from Mycobacterium tuberculosis, for skin test reagents. Tubercle Lung Dis. 77:250256.
80. Young, D. B.,, and T. R. Garbe. 1991. Lipoprotein antigens of Mycobacteriun tuberculosis. Res. Microbiol. 142:5565.

Tables

Generic image for table
Table 1

Reactivity of smear-positive and smear-negative TB patients with recombinant antigens

Citation: Laal S, Skeiky Y. 2005. Immune-Based Methods, p 71-83. In Cole S, Eisenach K, McMurray D, Jacobs, Jr. W (ed), Tuberculosis and the Tubercle Bacillus. ASM Press, Washington, DC. doi: 10.1128/9781555817657.ch5
Generic image for table
Table 2

Reactivity of HIV-infected TB patients with recombinant antigens

Citation: Laal S, Skeiky Y. 2005. Immune-Based Methods, p 71-83. In Cole S, Eisenach K, McMurray D, Jacobs, Jr. W (ed), Tuberculosis and the Tubercle Bacillus. ASM Press, Washington, DC. doi: 10.1128/9781555817657.ch5
Generic image for table
Table 3

T-cell antigens for use in diagnosis

Citation: Laal S, Skeiky Y. 2005. Immune-Based Methods, p 71-83. In Cole S, Eisenach K, McMurray D, Jacobs, Jr. W (ed), Tuberculosis and the Tubercle Bacillus. ASM Press, Washington, DC. doi: 10.1128/9781555817657.ch5

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