Chapter 9 : Introduction to Functional Genomics of the Complex

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Introduction to Functional Genomics of the Complex, Page 1 of 2

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This chapter starts with a description of the three complete genome sequences of H37Rv, CDC 1551, and AF2122/97 and highlights the genomic differences between members of the complex. Tremendous large-scale efforts have now led to the sequences of numerous genomes ranging from simple bacteria to complex organisms such as humans. has the metabolic potential to adapt to aerobic and microaerophilic as well as anaerobic environments. , the bovine tubercle bacillus, has the broadest host tropism, and this includes humans. The greatest variation between and has been identified among cell wall components and secreted proteins. The availability of the complete genome sequence of , combined with major progress in transcriptome technology, has led to the identification of a number of virulence genes. The classical proteome approach comprises protein separation by two-dimensional gel electrophoresis (2-DE) and spot identification by mass spectrometry or any biochemical or chemical cleavage techniques such as Edman degradation or enzymatic proteolysis. The goal of structural genomics is to apply high-throughput technologies to generate large amounts of recombinant proteins of for structure determination. Comparative genomics has provided insight into the coevolution of tubercle bacilli and their mammalian hosts, and one finding of some concern is the great diversity in the sequences of the PE and PPE proteins between strains.

Citation: Brodin P, Demangel C, Cole S. 2005. Introduction to Functional Genomics of the Complex, p 141-154. In Cole S, Eisenach K, McMurray D, Jacobs, Jr. W (ed), Tuberculosis and the Tubercle Bacillus. ASM Press, Washington, DC. doi: 10.1128/9781555817657.ch9

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Elongation Factor Tu
Cell Wall Components
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Figure 1

Distribution of the 4,043 genes in H37Rv according to their function.

Citation: Brodin P, Demangel C, Cole S. 2005. Introduction to Functional Genomics of the Complex, p 141-154. In Cole S, Eisenach K, McMurray D, Jacobs, Jr. W (ed), Tuberculosis and the Tubercle Bacillus. ASM Press, Washington, DC. doi: 10.1128/9781555817657.ch9
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Figure 2.

Functional genomics scheme showing information flow from the genome to applications. The URLs of useful websites are indicated.

Citation: Brodin P, Demangel C, Cole S. 2005. Introduction to Functional Genomics of the Complex, p 141-154. In Cole S, Eisenach K, McMurray D, Jacobs, Jr. W (ed), Tuberculosis and the Tubercle Bacillus. ASM Press, Washington, DC. doi: 10.1128/9781555817657.ch9
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