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Chapter 8 : Bacterial Biofilms on Plants: Relevance and Phenotypic Aspects

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Abstract:

Numerous bacteria and fungi are able to colonize and form biofilms on plant surfaces. Microbes colonizing aerial surfaces, such as flowers, stems, and leaves (phyllosphere), are generally referred to as epiphytes. Bacterial colonizers of the root surface (rhizosphere) are generally referred to as rhizobacteria. This chapter specifically focuses on the mechanisms involved in bacterial biofilm formation on plant roots, including pathogenic and beneficial interactions. Scanning electron microscopy (SEM) is a powerful tool to visualize at the single-cell level the process of colonization and microcolonies in the rhizosphere and on sprouts showing the presence of many differently shaped bacteria. The genetic background of plants should also be taken into account to gain a better understanding about how bacteria function in the rhizosphere. Quorum-sensing genes have been identified in several plant-associated bacteria regulating a large variety of different phenotypes involved in pathogenicity and plant beneficial effects. Hyphae of soil-born pathogenic fungi attach to the root and usually colonize the root surface before penetrating and infecting the root. Biofilm formation on fungal hyphae implicates the possibility of interactions at the molecular level between the fungus and the bacterium. Bacterium-fungus interactions are found widespread through nature, associated with plants and animals, where these microorganisms compete with each other, attack each other, and form consortia.

Citation: Bloemberg G, Lugtenberg B. 2004. Bacterial Biofilms on Plants: Relevance and Phenotypic Aspects, p 141-159. In Ghannoum M, O'Toole G (ed), Microbial Biofilms. ASM Press, Washington, DC. doi: 10.1128/9781555817718.ch8
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