1887

Section 4 : Bacterial Diagnosis

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $7.00

Preview this chapter:
Zoom in
Zoomout

Bacterial Diagnosis, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555817725/9781555812881_Chap04-1.gif /docserver/preview/fulltext/10.1128/9781555817725/9781555812881_Chap04-2.gif

Abstract:

This section provides guidelines for the selection and processing of specimens for the detection of specific bacteria. Testing can be subdivided into microscopy, culture, antigen tests (including immunoassays and molecular diagnostic tests), and antibody tests. Although it is impossible to provide guidelines for all possible infections, the most common bacteria associated with human disease are included. The section has been expanded to include summary tables of identification tests as well as a more detailed discussion of the immunological detection of organisms where appropriate. Tables have been provided that offer recommendations for gram stain and plating media, and list screening specimens for routine bacterial culture and processing specimens for mycobacterial identification. Microscopy includes acridine orange stain, auramine-rhodamine stain, direct fluorescent-antibody stain and gram stain, kinyoun stain. Primary plating media of bacteria includes bacteroides bile-esculin (BBE) agar, blood agar and brilliant green agar. A large number of media have been developed for the selective isolation of Campylobacter spp. from stool specimens. Most contain a brucella basal medium, which preferentially supports the growth of Campylobacter spp. Primary plating media of Mycobacteria includes American thoracic society medium, dubos broth, middlebrook 7H11 agar and petragnani medium. Specific diagnostic tests performed for aerobic gram-positive cocci, aerobic gram-positive rods, acid-fast and partially acid-fast gram-positive rods have been listed in a table. General comments about molecular methods for the identification of bacteria are discussed in the section.

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

References

/content/book/10.1128/9781555817725.chap4

Tables

Generic image for table
Table 4.1

Detection methods for bacteria a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.2

Recommendations for Gram stain and plating media a,b

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.3

Screening specimens for routine bacterial culture a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.4

Processing specimens for mycobacterial identification

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.5

Differential characteristics of catalase-positive gram-positive cocci

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.6

Differential characteristics of common Staphylococcus species

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.7

Differential characteristics of catalase-negative gram-positive cocci

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.8

Differential characteristics of beta-hemolytic streptococci a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.9

Differential characteristics of viridans streptococci

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.10

Differential characteristics of common Enterococcus species

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.11

Differential characteristics of gram-positive rods

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.12

Differential characteristics of selected Corynebacterium species

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.13

Differential characteristics of selected coryneform bacteria

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.14

Differential characteristics of selected Bacillus species and related genera

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.15

Differential characteristics of selected actinomycetes a,b

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.16

Differential characteristics of selected Nocardia species a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.17

Differential characteristics of selected weakly acid-fast Rhodococcus, Gordonia, and Tsukamurella species

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.18

Differential characteristics of nonchromogenic, slow-growing Mycobacterium species a,b

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.19

Differential characteristics of chromogenic, slow-growing Mycobacterium species a,b

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.20

Differential characteristics of rapidly growing Mycobacterium species a,b

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.21

Carbohydrate utilization tests of common rapidly growing Mycobacterium species a,b

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.22

Differential characteristics of the gram-negative Neisseria and Moraxella species

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.23

Differential characteristics of selected members of the Neisseriaceae

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.24

Differential characteristics of selected Actinobacillus, Haemophilus, and Pasteurella species

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.25

Differential characteristics of selected Capnocytophaga, Dysgonomonas, Chromobacterium, and Streptobacillus species

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.26

Differential characteristics of Haemophilus species

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.27

Differential characteristics of H. aphrophilus, H. paraphrophilus, and some related species a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.28

Differential characteristics of selected members of the Enterobacteriaceae

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.29

Differential characteristics of Citrobacter species a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.30

Differential characteristics of Enterobacter and Pantoea species a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.31

Differential characteristics of Klebsiella and Raoultella speciesª

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.32

Differential characteristics of Proteus, Providencia, and Morganella species a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.33

Differential characteristics of Yersinia species after incubation at 25 °C for 48 h a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.34

Differential characteristics of Aeromonas species a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.35

Differential characteristics of Vibrio species a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.36

Differential characteristics of Pseudomonas species a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.37

Differential characteristics of Ralstonia species a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.38

Differential characteristics of Brevundimonas, Delftia, Comamonas, and Stenotrophomonas species a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.39

Differential characteristics of selected nonfermentative gram-negative rods a

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.40

Differential characteristics of selected oxidase-negative, oxidative, gram-negative rods

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.41

Differential characteristics of selected oxidase-positive, oxidative, gram-negative rods

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.42

Differential characteristics of selected oxidase-positive, nonoxidative, gram-negative rods

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.43

Differential characteristics of selected Campylobacter, Arcobacter, and Helicobacter species

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.44

Differential characteristics of non-spore-forming, anaerobic, gram-positive rods

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.45

Differential characteristics of selected Actinomyces and Propionibacterium species

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.46

Differential characteristics of selected Clostridium species

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.47

Differential characteristics of anaerobic gram-negative bacteria

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4
Generic image for table
Table 4.48

Differential characteristics of the Bacteroides fragilis group

Citation: Murray P, Shea Y. 2004. Bacterial Diagnosis, p 129-232. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch4

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error