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Section 6 : Fungal Diagnosis

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Fungal Diagnosis, Page 1 of 2

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Abstract:

This section talks about fungal diagnosis and mycology specimen collection and transport guidelines. Table 6.1 lists methods for the identification of fungi. Microscopy includes acridine orange stain, Calcofluor white Stain, fluorescent-antibody stain and Kinyoun Stain. Table 6.2 includes characteristic fungal elements seen by direct examination of clinical specimens. Primary plating media includes birdseed agar, brain heart infusion Agar (BHI), dermatophyte test medium (DTM) and mycosel (mycobiotic) agar. Table 6.3 presents mycology plating guide. Specific diagnostic tests were performed for different species which includes Aspergillus Species, Blastomyces dermatitidis and Candida species. Microscopy and culture are sensitive detection methods for Aspergillus species. Molecular methods are being developed for both direct specimen and Aspergillus spp. identification. Table 6.4 provides cultural and biochemical characteristics of yeasts frequently isolated from clinical specimens. Table 6.5 offers characteristics of selected Trichosporon species. Table 6.6 lists characteristics of Aspergillus species. Table 6.7 is on opportunistic moniliaceous fungi.

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Tables

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Table 6.1

Methods for the identification of fungi

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.2

Characteristic fungal elements seen by direct examination of clinical specimens

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.3

Mycology plating guide

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.4

Cultural and biochemical characteristics of yeasts frequently isolated from clinical specimens a,b

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.5

Characteristics of selected Trichosporon species a

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.6

Characteristics of Aspergillus species a

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.7

Opportunistic moniliaceous fungi a

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.8

Zygomycetes a

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.9

Dimorphic moulds a

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.10

Characteristics of common Trichophyton species a

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.11

Epidermophyton floccosum and common Microsporum species a

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.12

Dematiaceous fungi with macroconidia or other structures a

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.13

Dematiaceous fungi with small conidia a

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.14

Differentiation of Cladosporium and Cladophialophora species a

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.15

Scedosporium and Dactylaria species a

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6
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Table 6.16

Dematiaceous fungi with hyphae a

Citation: Murray P, Shea Y. 2004. Fungal Diagnosis, p 255-302. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch6

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