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Section 7 : Parasitic Diagnosis

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Parasitic Diagnosis, Page 1 of 2

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Abstract:

Diagnosis of most parasitic infections has traditionally been made by the microscopic examination of clinical material. This requires that highly trained technologists spend a significant amount of time examining individual specimens. In recent years, immunoassays have been developed to detect the more common parasites (e.g., Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum). This section summarizes the tests currently available for the laboratory diagnosis of the most common parasitic infections. Table 7.1 lists detection methods for parasites. Microscopy contains different Stains which includes acid-fast trichrome chromotrope stain, calcofluor white stain and delafield's hematoxylin stain. Specific diagnostic tests were performed for free-living amebae, intestinal and urogenital protozoa, blood and tissue protozoa. Table 7.2 includes trophozoites of common intestinal amebae. Table 7.3 presents cysts of common mtestinal amebae. Table 7.4 is on trophozoites of flagellates. Table 7.5 is about cysts of flagellates. Table 7.6 offers morphological characteristics of ciliates, coccidia, microsporidia, and tissue protozoa. Table 7.7 lists morphological characteristics of protozoa found in blood. Table 7.8 provides morphological characteristics of blood and tissue nematodes. Table 7.9 is on morphological characteristics of helminths. Table 7.10 is key to identification of common arthropods.

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7
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Figures

Image of Figure 7.1
Figure 7.1

Intestinal amebae of humans. (Top row) Trophozoites. (Middle row) Cysts. (Bottom row) Trophozoite nuclei, shown in relative proportion. From P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed., ASM Press, Washington, D.C., 2003.

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7
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Image of Figure 7.2
Figure 7.2

Intestinal and urogenital flagellates of humans. (Top row) Trophozoites. (Bottom row) Cysts. D. fragilis trophozoite is shown; no cyst stage. From P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed., ASM Press, Washington, D.C., 2003.

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7
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Image of Figure 7.3
Figure 7.3

Relative sizes of helminth eggs (from CDC). Schistosoma mekongi and Schistosoma intercalatum have been omitted. From M. Brooke and D. Melvin, Morphology of Diagnostic Stages of Intestinal Parasites of Humans, 2nd ed., U.S. Department of Health and Human Services publication (CDC) 84-8116, Centers for Disease Control and Prevention, Atlanta, Ga., 1984.

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7
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References

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Tables

Generic image for table
Table 7.1

Detection methods for parasites a

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7
Generic image for table
Table 7.2

Trophozoites of common intestinal amebae a

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7
Generic image for table
Table 7.3

Cysts of common intestinal amebae a

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7
Generic image for table
Table 7.4

Trophozoites of flagellates a

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7
Generic image for table
Table 7.5

Cysts of flagellates a

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7
Generic image for table
Table 7.6

Morphological characteristics of ciliates, coccidia, microsporidia, and tissue protozoa a

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7
Generic image for table
Table 7.7

Morphological characteristics of protozoa found in blood a

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7
Generic image for table
Table 7.8

Morphological characteristics of blood and tissue nematodes

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7
Generic image for table
Table 7.9

Morphological characteristics of helminths a

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7
Generic image for table
Table 7.10

Key to identification of common arthropods a

Citation: Murray P, Shea Y. 2004. Parasitic Diagnosis, p 303-343. In Pocket Guide to Clinical Microbiology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817725.ch7

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