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Chapter 20 : Virulence Plasmids of : Characteristics and Comparison

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Abstract:

This chapter focuses on virulence plasmids of species with particular emphasis on their architecture, the virulence factors encoded by them, and comparisons between species and strains where possible. The involvement of plasmids in the virulence of has long been known and is well established. The best-characterized of these is the low calcium response (LCR) plasmid that is common to the three pathogenic species. The synthesis and regulation of effector Yop secretion by the type III system are extremely complex. The largest difference between the pLcr molecules that have been sequenced to date is in the position and orientation of some of the more important genes. The common mechanism of partitioning between the sequenced pLcr plasmids is interesting because the common partitioning system has been shown to be responsible for plasmid incompatibility between F and pYVe. The chapter addresses the putative virulence factors encoded by pFra as well as make postgenomic sequence comparisons where possible. The most prominent phage element that can be identified easily as a remnant on pFra is a portion of a lambda-like phage element. Recently, two independent self-transmissible antibiotic resistance plasmids (RTFs) have been identified in natural isolates of in Madagascar.

Citation: Lindler L. 2004. Virulence Plasmids of : Characteristics and Comparison, p 423-438. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch20

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Mobile Genetic Elements
0.5825865
Bacterial Pathogenesis
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Tumor Necrosis Factor alpha
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Type III Secretion System
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Figures

Image of Figure 1
Figure 1

Map of the common 26-kb core region encoding the type III secretion system of the virulence plasmid. Genes and direction of transcription are indicated by arrows. Genes and alternate names used in the different species are labeled with each coding region. The and designations were originally used for three separate loci identified by study fusions in ( ). The designation is used in ( ). The loci divide the majority of the core region (excluding into three parts. The locus extends from to The region extends from to The locus includes the through in

Citation: Lindler L. 2004. Virulence Plasmids of : Characteristics and Comparison, p 423-438. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch20
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Image of Figure 2
Figure 2

Comparative gene maps of the three sequenced pLcr plasmids. The sequences have been oriented such that the replication region is at the top of the map and the LCR core gene cluster is placed first proceeding clockwise around the circle. Only genes of interest are shown, with arrows indicating the direction of transcription. The closed dots indicate the position of the sequence GTATT. The locations of IS elements or remnants (designated with a “-r"” suffix) arc also shown and discussed in the text. Regions I, II, and III shown on the pCDl map are discussed in the text.

Citation: Lindler L. 2004. Virulence Plasmids of : Characteristics and Comparison, p 423-438. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch20
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Image of Figure 3
Figure 3

Gene map of the replication region of pCD1/pYVe227 and pYVc8081. The nomenclature of the genes is in keeping with the reports describing these plasmids ( ). Although the locations of these genes are quite similar, the level of homology at the DNA and protein level is quite low (see text). Arrows indicate the direction of transcription. The numbers indicate base-pair positions in the DNA sequence for pCDl (GenBank accession no. AF074612) and pYVe8081 (GenBank accession no. AF336309). The region includes the putative DnaA box and short repeat sequences generally found in plasmid origins.

Citation: Lindler L. 2004. Virulence Plasmids of : Characteristics and Comparison, p 423-438. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch20
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Image of Figure 4
Figure 4

Comparative architecture of the murine toxin plasmid pMTl/pFra derived from GenBank sequence AF074611 ( ). The black regions represent sequences in common with the cryptic serovar Typhi plasmid pHCM2 ( ). The light gray region represents DNA sequences that are absent in the CO92 plasmid pFra (GenBank accession no. AL117211). Genes and additional regions of interest are shown inside the circular map. The position of ORF123 is specifically shown because it shares 9 1% amino acid identity with ORFL7074 found on p0157 ( ). Only intact IS elements are shown. Restriction sites for Rland dIII are also shown.

Citation: Lindler L. 2004. Virulence Plasmids of : Characteristics and Comparison, p 423-438. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch20
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References

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1. Achtman, M.,, K. Zurth,, G. Morelli,, G. Torrea,, A. Guiyoule,, and E. Carniel. 1999. Yersinia pestis, the cause of plague, is a recently emerged clone of Yersinia pseudotuberculosis. Proc. Natl. Acad. Sci. USA 96:1404314048.
2. Anderson, G. W.Jr.,, P. L. Worsham,, C. R. Bolt,, G. P. Andrews,, S. L. Welkos,, A. M. Friedlander,, and J. P. Burans. 1997. Protection of mice from fatal bubonic and pneumonic plague by passive immunization with monoclonal antibodies against the Fl protein of Yersinia pestis. Am. J. Trop. Med. Hyg. 56:471473.
3. Andrews, G. P.,, D. G. Heath,, G. W. Anderson Jr.,, S. L. Welkos,, and A. M. Friedlander. 1996. Fraction 1 capsular antigen (Fl) purification from Yersinia pestis CO92 and from an Escherichia coli recombinant strain and efficacy against lethal plague challenge. Infect. Immun. 64:21802187.
4. Bakour, R.,, Y. Larochc,, and G. R. Cornelis. 1983. Study of the incompatibility and replication of the 70-kb virulence plasmid of Yersinia. Plasmid 10:279289.
5. Bick, D. P.,, and J. Shi. 1994. A single 43-bp sopC repeat of plasmid mini-F is sufficient to allow assembly of a functional nucleoprotein partition complex. Proc. Natl. Acad. Sci. USA 91:80278031.
6. Biot, T.,, and G. R. Cornelis. 1988. The replication, partition and yop regulation of the pYV plasmids are highly conserved in Yersinia enterocolitica and Y. pseudotuberculosis. J. Gen. Microbiol. 134:15251534.
7. Black, D. S.,, and J. B. Bliska. 2000. The RhoGAP activity of the Yersinia pseudotuberculosis cytotoxin YopE is required for antiphagocytic function and virulence. Mol. Microbiol. 37:515527.
8. Bolin, I.,, and H. Wolf-Watz. 1988. The plasmid-encoded Yop2b protein of Yersinia pseudotuberculosis is a virulence determinant regulated by calcium and temperature at the level of transcription. Mol. Microbiol. 2:237245.
9. Brubaker, R. R. 1991. Factors promoting acute and chronic diseases caused by yersiniae. Clin. Microbiol. Rev. 4:309324.
10. Brubaker, R. R. 1972. The genus Yersinia: biochemistry and genetics of virulence. Curr. Top. Microbiol. Immunol. 57:111158.
11. Brubaker, R. R. 1983. The Vwa + virulence factor of yersiniae: the molecular basis of the attendant nutritional requirement for Ca+ +. Rev. Infect. Dis. 5(Suppl. 4):S748S758.
12. Butler, T. 1983. Plague and Other Yersinia Infections. Plenum Medical Book Co., New York, N.Y..
13. Comelis, G. R.,, A. Boland,, A. P. Boyd,, C. Geuijen,, M. Iriarte,, C. Neyt,, M. P. Sory,, and I. Stainier. 1998. The virulence plasmid of Yersinia, an antihost genome. Microbiol. Mol. Biol. Rev. 62:13151352.
14. Cornelis, G. R.,, and F. Van Gijsegem. 2000. Assembly and function of type III secretory systems. Annu. Rev. Microbiol. 54:735774.
15. Cowan, S. T., 1974. In R. E. Buchanan, and N. E. Gibbons (ed.), Bergey's Manual of Determinitive Bacteriology, 8th ed. The Williams and Wilkins Co., Baltimore, Md..
16. DelSolar, G.,, R. Giraldo,, M. J. Ruiz-Echevarria,, M. Espinosa,, and R. Diaz-Orejas. 1998. Replication and control of circular bacterial plasmids. Microbiol. Mol. Biol. Rev. 62:434464.
17. Deng, W.,, V. Burland,, G. Plunkett,, A. Boutin,, G. F. Mayhcw,, P. Liss,, N. T. Perna,, D. J. Rose,, B. Mau,, D. C. Schwartz,, S. Zhou,, J. D. Fetherston,, L. E. Lindler,, R. R. Brubaker,, G. V. Piano,, S. C Straley,, K. A. McDonough,, M. L. Nilles,, J. S. Matson,, F. R. Blattner,, and R. D. Perry. 2002. Genome sequence of Yersinia pestis KIM. J. Bacteriol. 184:46014611.
18. Dennis, D. T.,, and J. M. Hughes. 1997. Multidrug resistance in plague. New Engl. J. Med. 337:702704.
19. Dong, X. Q.,, L. E. Lindler,, and M. C. Chu. 2000. Complete DNA sequence and analysis of an emerging cryptic plasmid isolated from Yersinia pestis. Plasmid 43:144148.
20. Dong, X. Q.,, H. B. Pen,, F. Ye,, J. H. Huang,, and D. Z. Yu. 1994. A molecular epidemiological study of plasmid DNA of Yerseinia pestis strains from plague foci in Yunnan, China. Endem. Dis. Bull. China 9:5863.
21. Drozdov, I. G.,, A. P. Anisimov,, S. V. Samoilova,, I. N. Yezhov,, S. A. Yeremin,, A. V. Karlyshev,, V. M. Krasilnikova,, and V. I. Kravchenko. 1995. Virulent non-capsulate Yersinia pestis variants constructed by insertion mutagenesis. J. Med. Microbiol. 42:264268.
22. Du, Y.,, E. Galyov,, and A. Forsberg. 1995. Genetic analysis of virulence determinants unique to Yersinia pestis. Contrib. Microbiol. Immunol. 13:321324.
23. Du, Y.,, R. Rosqvist,, and A. Forsberg. 2002. Role of fraction 1 antigen of Yersinia pestis in inhibition of phagocytosis. Infect. Immun. 70:14531460.
24. Filippov, A. A.,, N. S. Solodovnikov,, L. M. Kookleva,, and O. A. Protsenko. 1990. Plasmid content in Yersinia pestis strains of different origin. FEMS Microbiol. Lett. 55:4548.
25. Frean, J. A.,, A. T. Capper,, A. Bryskier,, and K. P. Klugman. 1996. In vitro activities of 14 antibiotics against 100 human isolates of Yersinia pestis from southern African plague focus. Antimicrob. Agents Chemother. 40:26462647.
26. Friedlander, A. M.,, S. L. Welkos,, P. L. Worsham,, G. P. Andrews,, D. G. Heath,, G. W. AndersonJr.,, M. L. Pitt,, J. Estep,, and K. Davis. 1995. Relationship between virulence and immunity as revealed in recent studies of the Fl capsule of Yersinia pestis. Clin. Infect. Dis. 21(Suppl. 2):S178S181.
27. Galimand, M.,, A. Guiyoule,, G. Gerbaud,, B. Rasoamanana,, S. Chanteau,, E. Carniel,, and P. Courvalin. 1997. Multidrug resistance in Yersinia pestis mediated by a transferable plasmid. N. Engl. J. Med. 337:677680.
28. Galyov, E. E.,, S. Hakansson,, A. Forsberg,, and H. Wolf-Watz. 1993. A secreted protein kinase of Yersinia pseudotuberculosis is an indispensable virulence determinant. Nature 361: 730732.
29. Galyov, E. E.,, S. Hakansson,, and H. Wolf-Watz. 1994. Characterization of the operon encoding the YpkA Ser/Thr protein kinase and the YopJ protein of Yersinia pseudotuberculosis. J. Bacteriol. 176:45434548.
30. Galyov, E. E.,, A. V. Karlishev,, T. V. Chernovskaya,, D. A. Dolgikh,, O. Smirnov,, K. I. Volkovoy,, V. M. Abramov,, and V. P. Zav'yalov, 1991. Expression of the envelope antigen Fl of Yersinia pestis is mediated by the product of caflM gene having homology with the chaperone protein PapD of Escherichia coli. FEBS Lett. 286:7982.
31. Galyov, E. E.,, O. Smirnov,, A. V. Karlishev,, K. I. Volkovoy,, A. I. Denesyuk,, I. V. Nazimov,, K. S. Rubtsov,, V. M. Abramov,, S. M. Dalvadyanz,, and V. P. Zav'yalov. 1990. Nucleotide sequence of the Yersinia pestis gene encoding Fl antigen and the primary structure of the protein. Putative T and B cell epitopes. FEBS Lett. 277:230232.
32. Gerdes, K.,, J. Moller-Jensen,, and R. Bugge Jensen. 2000. Plasmid and chromosome partitioning: surprises from phylogeny. Mol. Microbiol. 37:455466.
33. Goguen, J. D.,, J. Yother,, and S. C. Straley. 1984. Genetic analysis of the low calcium response in Yersinia pestis mu dl(Ap lac) insertion mutants. J. Bacteriol. 160:842848.
34. Guiyoule, A.,, G. Gerbaud,, C. Buchrieser,, M. Galimand,, L. Rahalison,, S. Chanteau,, P. Courvalin,, and E. Carniel. 2001. Transferable plasmid-mediated resistance to streptomycin in a clinical isolate of Yersinia pestis. Emerg. Infect. Dis. 7:4348.
35. Hayes, F.,, and S. J. Austin. 1993. Specificity determinants of the PI and P7 plasmid centromere analogs. Proc. Natl. Acad. Sci. USA 90:92289232.
36. Hinnebusch, B. J.,, M. L. Rosso,, T. G. Schwan,, and E. Carniel. 2002. High-frequency conjugative transfer of antibiotic resistance genes to Yersinia pestis in the flea midgut. Mol. Microbiol. 46:349354.
37. Hinnebusch, B. J.,, A. E. Rudolph,, P. Cherepanov,, J. E. Dixon,, T. G. Schwan,, and A. Forsberg. 2002. Role of Yersinia murine toxin in survival of Yersinia pestis in the midgut of the flea vector. Science 296:733735.
38. Hinnebusch, J.,, P. Cherepanov,, Y. Du,, A. Rudolph,, J. D. Dixon,, T. Schwan,, and A. Forsberg. 2000. Murine toxin of Yersinia pestis shows phospholipase D activity but is not required for virulence in mice. Int. J. Med. Microbiol. 290:483487.
39. Hu, P.,, J. Elliott,, P. McCready,, E. Skowronski,, J. Garnes,, A. Kobayashi,, R. R. Brubaker,, and E. Garcia. 1998. Structural organization of virulence-associated plasmids of Yersinia pestis. J. Bacteriol. 180:51925202.
40. Iriarte, M.,, and G. R. Cornelis,. 1999. The 70-kilobase virulence plasmid of Yersinia, p. 91126. In J. B. Kaper, and J. Hacker (ed.), Pathogenicity Islands and Other Mobile Virulence Elements. ASM Press, Washington, D.C..
41. Iriarte, M.,, and G. R. Cornelis. 1998. YopT, a new Yersinia Yop effector protein, affects the cytoskeleton of host cells. Mol. Microbiol. 29:915929.
42. Karlyshev, A. V.,, E. E. Galyov,, V. M. Abramov,, and V. P. Zav'yalov. 1992. CaflR gene and its role in the regulation of capsule formation of Y. pestis. FEBS Lett. 305:3740.
43. Karlyshev, A. V.,, E. E. Galyov,, O. Smirnov,, A. P. Guzayev,, V. M. Abramov,, and V. P. Zav'yalov. 1992. A new gene of the f1 operon of Y. pestis involved in the capsule biogenesis. FEBS Lett. 297:7780.
44. Lee, V. T.,, and O. Schneewind. 1999. Type III secretion machines and the pathogenesis of enteric infections caused by Yersinia and Salmonella spp. Immunol. Rev. 168:241255.
45. Leung, K. Y.,, B. S. Reisner,, and S. C. Straley. 1990. YopM inhibits platelet aggregation and is necessary for virulence of Yersinia pestis in mice. Infect. Immun. 58:32623271.
46. Leung, K. Y.,, and S. C. Straley. 1989. The yopM gene of Yersinia pestis encodes a released protein having homology with the human platelet surface protein GPIb alpha. J. Bacteriol. 171:46234632.
47. Lindler, L. E.,, G. V. Piano,, V. Burland,, G. F. Mayhew,, and F. R. Blattner. 1998. Complete DNA sequence and detailed analysis of the Yersinia pestis KIM5 plasmid encoding murine toxin and capsular antigen. Infect. Immun. 66:57315742.
48. McDonough, K. A.,, A. M. Barnes,, T. J. Quan,, J. Montenieri,, and S. Falkow. 1993. Mutation in the pla gene of Yersinia pestis alters the course of the plague bacillus-flea (Siphonaptera: Ceratopbyllidae) interaction. J. Med. Entomol. 30:772780.
49. Montie, T. C. 1981. Properties and pharmacological action of plague murine toxin. Pharmacol. Ther. 12:491499.
50. Moore, R. L.,, and R. R. Brubaker. 1975. Hybridization and deoxyribonucleotide sequences of Yersinia enterocolitica and other selected members of Enterobacteriaceae. Int. J. Syst. Bacteriol. 25:336339.
51. Mulder, B.,, T. Michiels,, M. Simonet,, M. P. Sory,, and G. Cornelis. 1989. Identification of additional virulence determinants on the pYV plasmid of Yersinia enterocolitica W227. Infect. Immun. 57:25342541.
52. Neyt, C.,, M. Iriarte,, V. H. Thi,, and G. R. Cornelis. 1997. Virulence and arsenic resistance in yersiniae. J. Bacteriol. 179:612619.
53. Osborn, M.,, S. Bron,, N. Firth,, S. Holsappcl,, A. Huddleston,, R. Kiewiet,, W. Meijer,, J. Seegers,, R. Skurry,, P. Terpstra,, C. M. Thomas,, P. Thorsted,, E. Tietze,, and S. L. Turner,. 2000. The evolution of bacterial plasmids, p. 301350. In C. M. Thomas (ed.), The Horizontal Gene Pool. Harwood Academic Publishers, Amsterdam, The Netherlands.
54. Pai, C.,, and V. Mors. 1978. Production of enterotoxin by Yersinia enterocolitica. Infect. Immun. 19:908911.
55. Parkhill, J.,, B. W. Wren,, N. R. Thomson,, R. W. Titball,, M. T. Holden,, M. B. Prentice,, M. Sebaihia,, K. D. James,, C. Churcher,, K. L. Mungall,, S. Baker,, D. Basham,, S. D. Bentley,, K. Brooks,, A. M. Cerdeno-Tarraga,, T. Chillingworth,, A. Cronin,, R. M. Davies,, P. Davis,, G. Dougan,, T. Feltwell,, N. Hamlin,, S. Holroyd,, K. Jagels,, A. V. Karlyshev,, S. Leather,, S. Moule,, P. C. Oyston,, M. Quail,, K. Rutherford,, M. Simmonds,, J. Skelton,, K. Stevens,, S. Whitehead,, and B. G. Barrell. 2001. Genome sequence of Yersinia pestisy the causative agent of plague. Nature 413:523527.
56. Pepe, J. C.,, and V. L. Miller. 1993. Yersinia enterocolitica invasin: a primary role in the initiation of infection. Proc. Natl. Acad. Sci. USA 90:64736477.
57. Perry, R. D.,, and J. D. Fetherston. 1997. Yersinia pestis— etiologic agent of plague. Clin. Microbiol. Rev. 10:3566.
58. Perry, R. D.,, S. C. Straley,, J. D. Fetherston,, D. J. Rose,, J. Gregor,, and F. R. Blattner. 1998. DNA sequencing and analysis of the low-Ca2+-response plasmid pCDl of Yersinia pestis KIM5. Infect. Immun. 66:46114623.
59. Portnoy, D. A.,, and S. Falkow. 1981. Virulence-associated plasmids from Yersinia enterocolitica and Yersinia pestis. J. Bacteriol. 148:877883.
60. Portnoy, D. A.,, and R. J. Martinez. 1985. Role of a plasmid in the pathogenicity of Yersinia species. Curr. Top. Microbiol. Immunol. 118:2951.
61. Portnoy, D. A.,, H. Wolf-Watz,, I. Bolin,, A. B. Beeder,, and S. Falkow. 1984. Characterization of common virulence plasmids in Yersinia species and their role in the expression of outer membrane proteins. Infect. Immun. 43:108114.
62. Prentice, M. B.,, K. D. James,, J. Parkhill,, S. G. Baker,, K. Stevens,, M. N. Simmonds,, K. L. Mungall,, C. Churcher,, P. C. Oyston,, R. W. Titball,, B. W. Wren,, J. Wain,, D. Pickard,, T. T. Hien,, J. J. Farrar,, and G. Dougan. 2001. Yersinia pestis pFra shows biovar-specific differences and recent common ancestry with a Salmonella enterica serovar Typhi plasmid. J. Bacteriol. 183:25862594.
63. Protsenko, O. A.,, P. I. Anisimov,, O. T. Mozharov,, N. P. Konnov,, and I. A. Popov, 1983. Detection and characterization of the plasmids of the plague microbe which determine the synthesis of pesticin I, fraction I antigen and "mouse" toxin exotoxin. Genetika 19:10811090.
64. Rosqvist, R.,, A. Forsberg,, M. Rimpilainen,, T. Bergman,, and H. Wolf-Watz. 1990. The cytotoxic protein YopE of Yersinia obstructs the primary host defence. Mol. Microbiol. 4:657667.
65. Rosqvist, R.,, K. E. Magnusson,, and H. Wolf-Watz. 1994. Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells. EMBO J. 13:964972.
66. Rudolph, A. E.,, J. A. Stuckey,, Y. Zhao,, H. R. Matthews,, W. A. Patton,, J. Moss,, and J. E. Dixon. 1999. Expression, characterization, and mutagenesis of the Yersinia pestis murine toxin, a phospholipase D superfamily member. J. Biol. Chem. 274:1182411831.
67. Samoilova, S. V.,, L. V. Samoilova,, I. N. Yezhov,, I. G. Drozdov,, and A. P. Anisimov. 1996. Virulence of pPst+ and pPst- strains of Yersinia pestis for guinea-pigs. J. Med. Microbiol. 45:440444.
68. Simpson, W. J.,, R. E. Thomas,, and T. G. Schvvan. 1990. Recombinant capsular antigen (fraction 1) from Yersinia pestis induces a protective antibody response in BALB/c mice. Am. J. Trap. Med. Hyg. 43:389396.
69. Smith, M. D.,, D. X. Vinh,, N. T. T. Hoa,, J. Wain,, D. Thung,, and N.J. White, 1995. In vitro antimicrobial susceptibilities of strain of Yersinia pestis. Antimicrob. Agents Chemother. 39:21532154.
70. Snellings, N. J.,, M. Popek,, and L. E. Lindler. 2001. Complete DNA sequence of Yersinia enterocolitica serotype 0:8 low-calcium- response plasmid reveals a new virulence plasmid-associated replicon. Infect. Immun. 69:46274638.
71. Sodeinde, O. A.,, Y. V. Subrahmanyam,, K. Stark,, T. Quan,, Y. Bao,, and J. D, Goguen. 1992. A surface protease and the invasive character of plague. Science 258:10041007.
72. Straley, S. C.,, and W. S. Bowmer. 1986. Virulence genes regulated at the transcriptional level by Ca2+ in Yersinia pestis include structural genes for outer membrane proteins. Infect. Immun. 51:445454.
73. Straley, S. C.,, and M. L. Cibull. 1989. Differential clearance and host-pathogen interactions of YopE- and YopK- YopL- Yersinia pestis in BALB/c mice. Infect. Immun. 57:12001210.
74. Titball, R. W.,, A. M. Howells,, P. C. Oyston,, and E. D. Williamson. 1997. Expression of the Yersinia pestis capsular antigen (Fl antigen) on the surface of an aroA mutant of Salmonella typhimurium induces high levels of protection against plague. Infect. Immun. 65:19261930.
75. Vanooteghem, J. C.,, and G. R. Cornelis. 1990. Structural and functional similarities between the replication region of the Yersinia virulence plasmid and the RepFIIA replicons. Bacteriol. 172:36003608.
76. Von Pawel-Rammingen, U.,, M. V. Tclepnev,, G. Schmidt,, K. Aktories,, H. Wolf-Watz,, and R. Rosqvist. 2000. GAP activity of the Yersinia YopE cytotoxin specifically targets the Rho pathway: a mechanism for disruption of actin microfilament structure. Mol. Microbiol. 36:737748.
77. Welkos, S. L.,, K. M. Davis,, L. M. Pitt,, P. L. Worsham,, and A. M. Freidlander. 1995. Studies on the contribution of the Fl capsule-associated plasmid pFra to the virulence of Yersinia pestis. Contrib. Microbiol. Immunol. 13:299305.
78. Welkos, S. L.,, A. M. Friedlander,, and K.J. Davis. 1997. Studies on the role of plasminogen activator in systemic infection by virulent Yersinia pestis strain C092. Microb. Pathog. 23:211223.
79. Worsham, P. L.,, M. P. Stein,, and S. L. Welkos. 1995. Construction of defined Fl negative mutants of virulent Yersinia pestis. Contrib. Microbiol. Immunol. 13:325328.
80. Youngren, B.,, L. Radnedge,, P. Hu,, E. Garcia,, and S. Austin. 2000. A plasmid partition system of the Pl-P7par family from the pMTl virulence plasmid of Yersinia pestis. J. Bacteriol. 182:39243928.

Tables

Generic image for table
Table 1

Molecular properties of plasmids

Citation: Lindler L. 2004. Virulence Plasmids of : Characteristics and Comparison, p 423-438. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch20
Generic image for table
Table 2

List of pLcr-encodcd established effector proteins and virulence properties

Citation: Lindler L. 2004. Virulence Plasmids of : Characteristics and Comparison, p 423-438. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch20

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