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Chapter 5 : Partition Systems of Bacterial Plasmids

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Abstract:

Plasmids, as extrachromosomal elements, bear the burden of ensuring their own faithful segregation at cell division. This chapter reviews partition systems, which are, in general, systems that actively dictate the specific localization of plasmids inside the bacterial cell and coordinate this localization with the bacterial cell cycle. Partition systems also exert incompatibility, which is distinct from the replication-mediated incompatibility that has been used to classify plasmids. Growth of the membrane between attachment sites was proposed to push plasmids apart. It was subsequently shown that membrane growth is dispersive and thus cannot solely account for plasmid movement. An appealing candidate for the plasmid road sign is the bacterial replication apparatus. Experiments in and indicate that the replication machinery exists as localized factories in the cell. The intracellular localization patterns of the ParA from the virulence plasmid pB171 provide an intriguing clue as to the mechanism of ParA function. RepA and RepB are not essential for replication but are essential for plasmid stability. They have been shown to influence copy number, but this may be due to effects on the expression of . Recent cell biology, biochemical, and structural data show that R1 ParM looks and behaves like actin and suggest a partition model in which ParM acts as a cytoskeletal element to drive the movement of plasmids during the cell cycle.

Citation: Funnell B, Slavcev R. 2004. Partition Systems of Bacterial Plasmids, p 81-104. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch5

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Figures

Image of Figure 1
Figure 1

General scheme depicting a plasmid partition reaction. Newly replicated plasmids at the cell center are separated and relocated to the quarter-cell positions prior to septum formation. These positions become the cell center in newly replicated daughter cells. The components of the apparatus tethering the plasmid to the cell (shown as dotted oval) are still unidentified.

Citation: Funnell B, Slavcev R. 2004. Partition Systems of Bacterial Plasmids, p 81-104. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch5
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Image of Figure 2
Figure 2

Genetic organization of plasmid partition loci, representing the five groups of partition systems that are discussed in the text. Bach group is labeled by one or two representative members (see Table 1 ). Genes encoding partition proteins are shown as black bars, whereas gray bars and text denote nonpartition genes that are cotranscribed with partition genes. Checkered boxes denote -acting genetic elements, i.e., operators and centromeres. The O O operators, depicted for the RK2 plasmid partition locus, are specifically bound by KorA and KorB, respectively. Arrows indicate promoters and the direction of transcription.

Citation: Funnell B, Slavcev R. 2004. Partition Systems of Bacterial Plasmids, p 81-104. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch5
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Image of Figure 3
Figure 3

Phylograms of partition ATPases (assembled using AlignX/Vector NT1 software; InforMax) ( ). Groups of similar sequences are labeled as in Table 1 . For plasmids encoding dual partition systems, the specific partition ATPase is listed after the plasmid. (A) Walker-type partitioning ATPases. Horizontal line lengths reflect relative evolutionary distances between 60 deviant Walker-type partitioning ATPases encoded on various plasmids (listed adjacent to phylogram), and branches are drawn proportional to the amount of inferred character change. (B) Actin-like partition ATPases. Horizontal lengths represent relative evolutionary distance between seven actin-like partition ATPases. The actin-like ATPases in (B) have no significant sequence similarity to the Walker-type ATPases listed in (A).

Citation: Funnell B, Slavcev R. 2004. Partition Systems of Bacterial Plasmids, p 81-104. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch5
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Image of Figure 4
Figure 4

Arrangement of protein sequence motifs in (A) partition ATPases and (B) centromere-binding proteins of partition systems that contain Walker-type partition ATPases. The motifs are described in the text. In (A), the shaded lines below the motif diagram indicate the regions carried on different ATPases. In (B), the short ParB/ParG proteins of the pTAR/TP228 group of plasmids are not members of this ParB protein family and are not included.

Citation: Funnell B, Slavcev R. 2004. Partition Systems of Bacterial Plasmids, p 81-104. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch5
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Figure 5

Organization of the P1 and F partition sites. In (A) and (B), checkered boxes represent partition sites downstream of the partition genes (gray rectangles). Arrows denote the direction of repeated sequences. (A) P1 , identifying the Box A (white boxes labeled A1 to A4) and Box B (black boxes labeled B1 and B2) motifs that are recognized by ParB, and the IHF binding site. (B) a set of twelve 43-bp repeats (large arrows) that each contain a smaller inverted repeat (smaller colliding arrows). (C) Alignment of the sequences of three known sites (P1, P7, and pMT1) with four putative sites (pSLT, 50K virulence plasmid, Rtsl, and pWR501) (see Table 1 for ParA, ParB, and species information). The known or predicted (from alignment with P1 and P7 sequences) ParB Box A and Box B motifs and IHF-binding sites are illustrated as in (A). Note that Boxes B1, B2, A2, and A3 are the only ParB motifs that are essential for formation of the high-affinity ParB-IHF complex at P1 ( ). In addition, although the pWR501 sequence has a 21-bp insertion between the IHF site and the right side of ( ), such insertions are permitted in P1 if they represent an integral number of turns of the DNA helix ( ). The accession numbers for these sequences are: P1, K02380; P7, X17529; pMT1, AF074611; pSLT, AE006471; P50K, AB040415; Rtsl, AP004237; pWR50l, NC_002698.

Citation: Funnell B, Slavcev R. 2004. Partition Systems of Bacterial Plasmids, p 81-104. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch5
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Tables

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Table 1

Partition ATPases and associated centromere-binding proteins

Citation: Funnell B, Slavcev R. 2004. Partition Systems of Bacterial Plasmids, p 81-104. In Funnell B, Phillips G (ed), Plasmid Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817732.ch5

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