1887

Chapter 2 : RecA-Dependent Mechanisms for the Generation of Genetic Diversity

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $30.00

Preview this chapter:
Zoom in
Zoomout

RecA-Dependent Mechanisms for the Generation of Genetic Diversity, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555817749/9781555812713_Chap02-1.gif /docserver/preview/fulltext/10.1128/9781555817749/9781555812713_Chap02-2.gif

Abstract:

This chapter discusses the roles of RecA and other proteins in homologous recombination. It also focuses on RecA's other roles, as these also contribute to increases in genetic diversity. It discusses how homologous recombination occurs at the molecular level and how this can lead to an increase in genetic diversity. It summarizes the current understanding of 's crucial and essential role in homologous recombination, its other roles in the regulation of damage-inducible DNA-repair pathways, and its participation in mutagenic events that can lead to increases in genetic diversity. It is most convenient to think of recombination as a three-stage process. The three stages are called presynapsis, synapsis, and postsynapsis. These stages are used to discuss the processing of the DNA substrates and the enzymes that act on them. Presynapsis describes the tailoring of the DNA substrates so that they can interact with the RecA protein. If the recombination enzymes and substrates are present, the enzymes will recombine any DNA that is available with great accuracy, precision, and efficiency. The prevailing thought in the chapter is that once recombination substrates are presented to a cell, given that the cell has the proper enzymes, recombination will occur. RecA aids in the repair of double-strand breaks in DNA.

Citation: Sandler S, Nüsslein K. 2004. RecA-Dependent Mechanisms for the Generation of Genetic Diversity, p 21-35. In Miller R, Day M (ed), Microbial Evolution. ASM Press, Washington, DC. doi: 10.1128/9781555817749.ch2

Key Concept Ranking

Bacterial Proteins
0.509419
DNA Polymerase V
0.5088613
0.509419
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

Figures

Image of FIGURE 1
FIGURE 1

Two different types of DNA substrates that could be formed as a result of a replication fork running into a nick (formation of a double-strand end) or a noncoding lesion (square on the DNA) to form a gap. The gene products and the particular stage of recombination at which they are predicted to function are indicated.

Citation: Sandler S, Nüsslein K. 2004. RecA-Dependent Mechanisms for the Generation of Genetic Diversity, p 21-35. In Miller R, Day M (ed), Microbial Evolution. ASM Press, Washington, DC. doi: 10.1128/9781555817749.ch2
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of FIGURE 2
FIGURE 2

A three-stranded reaction, (a) RecA initially binds the ssDNA, creating a protein DNA helical filament, (b) A duplex of DNA then interacts in the major groove of the filament, (c) One strand is then exchanged for the other, (d and e) Completion of the reaction. (This figure is reprinted from with permission of the authors.)

Citation: Sandler S, Nüsslein K. 2004. RecA-Dependent Mechanisms for the Generation of Genetic Diversity, p 21-35. In Miller R, Day M (ed), Microbial Evolution. ASM Press, Washington, DC. doi: 10.1128/9781555817749.ch2
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of FIGURE 3
FIGURE 3

Model of a filament of RecA at the molecular level. Twenty-four monomers of the RecA crystal structure have been assembled in a filament. One monomer is shown in darker shading. (This figure is reprinted from with permission of the authors.)

Citation: Sandler S, Nüsslein K. 2004. RecA-Dependent Mechanisms for the Generation of Genetic Diversity, p 21-35. In Miller R, Day M (ed), Microbial Evolution. ASM Press, Washington, DC. doi: 10.1128/9781555817749.ch2
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of FIGURE 4
FIGURE 4

Formation of a Holliday junction, its isomerization between the crossover and open-planar forms, and its resolution into patched and crossover recombinants. The arrows pointing to the strands in the Holliday junction are indicative of the strands cleaved by RuvC. Note that pairs of strands are cleaved to form the recombinants. The letters provide orientation for how the arms of the structure are rotated in space. The double arrows indicate isomerization.

Citation: Sandler S, Nüsslein K. 2004. RecA-Dependent Mechanisms for the Generation of Genetic Diversity, p 21-35. In Miller R, Day M (ed), Microbial Evolution. ASM Press, Washington, DC. doi: 10.1128/9781555817749.ch2
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of FIGURE 5
FIGURE 5

Replication fork reversal. The two differently shaded strands indicate parental and newly synthesized DNA. The enzymes that are known to catalyze this reaction are shown at the sides. Although only RecG is mentioned in the text as an enzyme that can perform this reaction, there is evidence that RuvAB also catalyzes this reaction. The letters provide orientation for how the arms of the structure are rotated in space. The double arrows indicate isomerization.

Citation: Sandler S, Nüsslein K. 2004. RecA-Dependent Mechanisms for the Generation of Genetic Diversity, p 21-35. In Miller R, Day M (ed), Microbial Evolution. ASM Press, Washington, DC. doi: 10.1128/9781555817749.ch2
Permissions and Reprints Request Permissions
Download as Powerpoint

References

/content/book/10.1128/9781555817749.chap2
1. Goodman, M. F. 2000. Coping with replication 'train wrecks' in Escherichia coli using PolV, PolII and RecA proteins. Trends Biochem. Sci. 25:189195.
2. Clark, A. J. 1996. recA mutants of E. coli K12: a personal turning point. BioEssays 18:767772.
3. Clark, A. J.,, and A. D. Margulies. 1965. Isolation and characterization of recombination deficient mutants of Escherichia coli K-12. Proc. Natl. Acad. Sci. USA 53:451459.
4. Cox, M. M. 2001. Historical overview: searching for replication help in all of the rec places. Proc. Nati. Acad. Sci. USA 98:81738180.
5. Howard-Flanders, P.,, and L. Theriot. 1966. Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics 53: 11371150.
6. Konrad, E. B. 1977. Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications. J. Bacteriol. 130:167172.
7. Lovett, S. T.,, and A. J. Clark. 1983. Genetic analysis of regulation of the RecF pathway of recombination in Escherichia coli K-12. J. Bacteriol. 153:14711478.
8. Lusetti, S. L.,, and M. M. Cox. 2002. The bacterial RecA protein and the recombinational DNA repair of stalled replication forks. Annu. Rev. Biochem. 71:71100.
9. Masters, M., 1996. Generalized transduction, p. 24212441. In F. C. Neidhardt (éd.), Escherichia coli and Salmonella: Cellular and Molecular Biology, vol. 2. ASM Press, Washington, D.C..
10. Nelson, D. L.,, and M. M. Cox. 2000. Lehninger Principles of Biochemistry. Worth Publishers, New York.
11. Sandler, S. J., 2001. Post-replication repair: a new perspective that focuses on the coordination between recombination and DNA replication, p. 2142. In M. F. Hoekstra, and J. A. Nickoloff (ed.), DNA Damage and Repair: Advances from Phage to Humans, vol. 3. Humana Press, Totowa, N.J..
12. Walker, G., 1996. The SOS response of Escherichia coli, p. 14001416. In F. C. Neidhardt,, R. Curtiss III,, J. L. Ingraham,, E. C. C. Lin,, K. B. Low,, B. Magasanik,, W. S. Reznikoff,, M. Riley,, M. Schaechter,, and H. E. Umbarger (ed.), Escherichia coli and Salmonella, Molecular and Cellular Biology, 2nd ed., vol. 1. American Society for Microbiology, Washington, D.C..

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error