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Chapter 13 : The PaJaMa Experiment

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The PaJaMa Experiment, Page 1 of 2

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Abstract:

The author undertook a systematic screening of the inducing capacity of all available thiogalactosides, of their affinity to the purified β-galactosidase, and of their “apparent affinity” to the intracellular enzyme. Constitutive strains, which synthesized β-galactosidase in media devoid of inducer, had been isolated. The prevailing hypothesis was that they accumulated an “internal” inducer. The availability of powerful inhibitors of induction prompted the author's group to check their ability to block the constitutive synthesis, with entirely negative results. The author attempted to map the operator gene. The bias was that the strains utilized were heterogeneous in the activity of a galactose transport system. The operator moved back to one end of the galactose operon. The striking similarity exhibited by the regulatory elements of the lactose and galactose systems strengthened both the repressor hypothesis and the notion that the unit of genetic expression can comprise several genes. Taking advantage of the fact that the lactose regulator gene is close enough to the genes it controls to also remain associated to them in the transducing phages, Salvador Luria could establish that the cointegration of an active gene was necessary and sufficient to prevent the derepression of the lactose enzymes during the phage vegetative growth. Unfortunately, the structure of the galactose system did not enable one to decide if the same explanation accounted entirely for the derepression of galactokinase. The switch "from lactose to galactose" induction analysis also supplied unexpected insights on the deregulation of host enzyme synthesis by viral induction.

Citation: Pardee A. 2003. The PaJaMa Experiment, p 133-142. In Ullmann A (ed), Origins of Molecular Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817763.ch13

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Messenger RNA
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Citation: Pardee A. 2003. The PaJaMa Experiment, p 133-142. In Ullmann A (ed), Origins of Molecular Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817763.ch13
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