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Chapter 6 : Le Labo de Jacques

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Le Labo de Jacques, Page 1 of 2

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Abstract:

On arriving at the Pasteur Institute, the author told Jacques Monod that he had come there to learn about adaptive enzymes and that he wished him to suggest a problem for him to work on. As a matter of fact, a strong candidate for a “preenzyme” had already been found in noninduced by Mel Cohn and Annamaria Torriani. Cohn and Torriani had shown that there was present in extracts from noninduced cells an enzymatically inactive protein which they called Pz. This protein cross-reacted with antibodies raised in rabbits by immunization with purified β-galactosidase (Gz). The experiment that Jacques proposed was indeed very simple. He had recently received a series of twenty auxotrophic mutants of from Bernard Davis, each requiring a different amino acid or accessory factor for growth. Enzyme synthesis began almost immediately upon adding back the required amino acid or growth factor. At this time, the kinetics of enzyme production were being followed as a function of time. It seemed to the author that if growth were indeed required, enzyme production should be measured with respect to the increase in bacterial mass. The final and complete elimination of the Pz protein as a precursor having anything to do with induction of β-galactosidase synthesis came a few years later when Cohn, Lennox, and Spiegelman transduced the Z gene into an iz containing no Pz, and obtained+ recombinant .

Citation: Torriani A. 2003. Le Labo de Jacques, p 55-62. In Ullmann A (ed), Origins of Molecular Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817763.ch6

Key Concept Ranking

Shigella dysenteriae
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Escherichia coli
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Glucose Oxidase
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Figures

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Figure 1

Luigi Gorini, Jacques Monod, Annamaria Torriani, and Mike Doudoroff, 1948 or 1949.

Citation: Torriani A. 2003. Le Labo de Jacques, p 55-62. In Ullmann A (ed), Origins of Molecular Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817763.ch6
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Figure 2

Citation: Torriani A. 2003. Le Labo de Jacques, p 55-62. In Ullmann A (ed), Origins of Molecular Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817763.ch6
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Citation: Torriani A. 2003. Le Labo de Jacques, p 55-62. In Ullmann A (ed), Origins of Molecular Biology. ASM Press, Washington, DC. doi: 10.1128/9781555817763.ch6
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