Chapter 8 : Culture-Dependent Microbiology

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This chapter concentrates on some examples of the latest techniques being successfully exploited for isolating dominant but uncultured bacteria from a variety of habitats. Regardless of what isolation method is used, the choice of an appropriate medium is crucial when culturing bacteria in nature. The most successful isolation approaches for numerically abundant types of bacteria by plating on solid media use very-low-nutrient media (5 to 80 mg of organic components per liter) incubated for up to 84 days. Plating has also been used to isolate new, abundant bacteria from some extreme environments. Enrichment has been especially successful at isolating naturally abundant thermophiles in the from a hot-subsurface aquifer from a gold mine. Micromanipulation can also be a valuable aid for isolating target bacteria, especially when fluorescent in situ hybridization (FISH) with phylogenetic probes is used to visualize the target bacteria. Finally, the chapter describes the extinction culture, which is also called dilution culture and dilution to extinction. It involves diluting water samples with filter-sterilized water until only a few bacteria remain and then growing the cells in either the unamended water or by adding small amounts of organic substrates to culture them.

Citation: Fry J. 2004. Culture-Dependent Microbiology, p 80-87. In Bull A (ed), Microbial Diversity and Bioprospecting. ASM Press, Washington, DC. doi: 10.1128/9781555817770.ch8

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Table 1

Some examples of methods used for the isolation of abundant from natural habitats

Citation: Fry J. 2004. Culture-Dependent Microbiology, p 80-87. In Bull A (ed), Microbial Diversity and Bioprospecting. ASM Press, Washington, DC. doi: 10.1128/9781555817770.ch8

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