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Color Plates

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Figures

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Color Plate 1

. Low-density, DNA microchip fingerprints. 16S rRNA from single bacterial targets and as cocultures (each vertical column) were labeled with a fluorescent dye (Texas red) and hybridized with the oligonucleotide probles indicated by numbered rows. Melting curves were determined on the stage of a custom-made epifluorescence microscope equipped with appropriate fluorescence filters and a cooled charge-coupled device camera and controlled by a computer for image acquisition (for more details, see Liu et al., 2001). High levels of hybridization are indicated by red to pink to white as indicated by the scale at the bottom of the chip (from Liu et al., 2001, with permission).

Citation: Bull A. 2004. Color Plates, In Microbial Diversity and Bioprospecting. ASM Press, Washington, DC.
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Color Plate 2

. Isolation of aquatic bacteria from the phylum (previously [CFB]) from the River Taff in Cardiff, Wales, by plating onto agar. All plates were incubated for 7 days at 20°C. (a) Bacterial colonies on CPS agar (low organic carbon, 2.5 g of organic compounds per liter). Some potential CFB isolates that were identified as yellow, orange, red, or spreading colonies are indicated by the yellow arrows. (b) Isolate EP293 growing as large yellow colonies on plate count agar (high organic carbon, 8.5 g liter), characterized by 16S rDNA sequencing as a possible new genus of the family most closely related phylogenetically to (93% sequence similarity). (c) Isolate EP233 growing as spreading, yellow colonies on CYT agar (loworganic carbon, 2 g liter), characterized by 16S rDNA sequencing as a possible new species of (family ), (d) Very small colonies (≤0.1 mm in diameter) growing on CPS agar (some indicated by white arrows). A large doughnut-shaped colony (3.3 mm in diameter; similar to the one indicated in panel a by a white arrow) gives a size comparison; bar, 1 mm. Taxonomic names are given according to the latest Bergey's taxonomic outline (Garrity et al., 2002). Panels b and c are used with permission from Louise A. O'Sullivan, Cardiff University.

Citation: Bull A. 2004. Color Plates, In Microbial Diversity and Bioprospecting. ASM Press, Washington, DC.
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Color Plate 5

. Composite maps can be constructed online in real time by clicking on the digital “layers” that one wants displayed. In this example, the base map, general locations, thermal features, major hydrography, lakes, and the boundary of YNP have been selected as the “visible” layers. Thermal features have been selected as the "active" layer from which simple queries can be performed.

Citation: Bull A. 2004. Color Plates, In Microbial Diversity and Bioprospecting. ASM Press, Washington, DC.
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Color Plate 6

. Multiple attributes can be used to search for springs that satisfy particular criteria. (A) A query was performed to search for springs that have a temperature greater than 80°C and a pH greater than 8. (B) The resulting data table lists all the springs that match these criteria. (C) A thermal spring (Octopus Spring) is selected from the list and the system zooms in on the location and launches a summary data table.

Citation: Bull A. 2004. Color Plates, In Microbial Diversity and Bioprospecting. ASM Press, Washington, DC.
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Color Plate 3

. Epifluorescence micrograph of cryosections of visualized by FISH with Cy3-labeled probes specific for (A) all bacteria and (B) the group. Details are given by Webster et al. (2001). The scale bars are 10 μm.

Citation: Bull A. 2004. Color Plates, In Microbial Diversity and Bioprospecting. ASM Press, Washington, DC.
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Color Plate 4

. Maps are constructed from multiple digital layers. Base maps can be overlaid with polygon data, which represent the location of lakes and geothermal basins, line data indicating the location of rivers, roads, streams, etc., and point data, which indicate the location of springs or sampling areas. In this example, the topographical map is overlaid with the location of rivers, roads, and thermal areas.

Citation: Bull A. 2004. Color Plates, In Microbial Diversity and Bioprospecting. ASM Press, Washington, DC.
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Color Plate 7

. The rcsulrs of a query, “Where have members of the genus been detected?” displayed in map format. Red dots indicate the locations where had been detected, and the size of the dot is proportional to the numbers of species or strains detected.

Citation: Bull A. 2004. Color Plates, In Microbial Diversity and Bioprospecting. ASM Press, Washington, DC.
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Color Plate 8

. Overview of metabolism and transport in . (From Gardner et al. (2002) with permission.)

Citation: Bull A. 2004. Color Plates, In Microbial Diversity and Bioprospecting. ASM Press, Washington, DC.
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Color Plate 9

. Presence of genes in the actinorhodin cluster of "" A3(2) in the core genes of members of the genome species.

Citation: Bull A. 2004. Color Plates, In Microbial Diversity and Bioprospecting. ASM Press, Washington, DC.
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Color Plate 10

. Increase in lettuce growth following treatment with at 0.1% (L) and 1% (H) (wt/wt) in the potting compost.

Citation: Bull A. 2004. Color Plates, In Microbial Diversity and Bioprospecting. ASM Press, Washington, DC.
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Color Plate 11

. Increase in marigold flowering following treatment with at 1% (wt/wt) in potting compost.

Citation: Bull A. 2004. Color Plates, In Microbial Diversity and Bioprospecting. ASM Press, Washington, DC.
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