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Chapter 10 : Mycobacteria

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Abstract:

Currently a number of automated commercially available rapid detection systems that can also perform antimycobacterial susceptibility testing are available to clinical laboratories. Further, several commercially available identification systems that utilize molecular methods are used by reference laboratories for the rapid identification of clinically important species of mycobacteria. One of the most important components of the laboratory diagnosis of mycobacterial infections is the use of the acidfast stain; either it is the auramine-rhodamine fluorescent stain or conventional carbol fuchsin-based stain. The Centers for Disease Control and Prevention emphasized the need for rapid turnaround times, not only for acid-fast smear results and recovery of mycobacteria from clinical specimens but also for subsequent antimicrobial susceptibility testing. The ESP Culture System II is an automated method that was originally devised for blood cultures and subsequently was adapted for the recovery of mycobacteria in clinical specimens. The MTD2 test is an isothermal transcriptionmediated amplification system based on specific mycobacterial rRNA targets using DNA intermediates. MicroSeq program technology promises to be the most accurate identification method and will probably be the standard for mycobacterial identification in the future. Presently, there are three antibodies available; two react with several mycobacterial species, including members of the complex. One antibody is thought to be specific for complex. Currently the gold standard still consists of traditional culture and antimicrobial susceptibility testing procedures. However, the authors feel that molecular methods will allow laboratories to detect, identify, and determine drug resistance of mycobacteria without culturing the organism.

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10

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Antimicrobial Susceptibility Testing
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Restriction Fragment Length Polymorphism
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FIGURE 1

Wescor Aerospray stainer.

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10
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FIGURE 2

Becton Dickinson BACTEC 960 MGIT system.

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10
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FIGURE 3

Roche COBAS AMPLICOR.

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10
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FIGURE 4

Abbott LCx system.

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10
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FIGURE 5

Becton Dickinson ProbeTec ET system.

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10
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Tables

Generic image for table
TABLE 1

Evaluations of MB Redox culturing system

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10
Generic image for table
TABLE 2

Evaluations of MGIT

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10
Generic image for table
TABLE 3

Evaluations of MB/BacT

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10
Generic image for table
TABLE 4

Evaluations of ESP Culture System II

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10
Generic image for table
TABLE 5

Evaluations of MTD2 Assay

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10
Generic image for table
TABLE 6

Evaluations of Roche AMPLICOR MTB test

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10
Generic image for table
TABLE 7

Evaluations of the LCx MTB assay

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10
Generic image for table
TABLE 8

Evaluations of BD ProbeTec ET System strand displacement assay

Citation: Roberts G, Hall L, Wolk D. 2002. Mycobacteria, p 256-273. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch10

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