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Chapter 17 : Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria

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Abstract:

Antimicrobial susceptibility testing (AST) can be performed by four basic methods: disk diffusion, broth dilution, agar dilution, and gradient diffusion. The authors recognize that susceptibility testing methods and panels are subject to periodic modifications and that new systems and panels are introduced into various global markets as an ongoing process. This chapter reviews recently published evaluations of currently used commercial systems. The automated and semiautomated systems discussed in the chapter have the capability of producing standardized or customized patient test reports generated by computer software packages that are referred to as data management systems (DMS). In the chapter, five current automated systems are discussed individually and evaluations (advantages and limitations) are presented as an overview of the current literature. The automated Sensititre ARIS system is a broth microdilution method utilizing a standard 96-microwell panel containing serial dilutions of dehydrated antimicrobial agents. Panel configurations are currently available for gram-positive and gram-negative bacteria in either a MIC-only format or a breakpoint format and are listed in the chapter. The chapter describes published evaluations of several commercial MIC methods for , and reviews several commercial methods for the rapid detection of methicillin-resistant (MRSA).

Citation: Evangelista A, Truant A. 2002. Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria, p 413-428. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch17

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Antimicrobial Susceptibility Testing
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beta-Lactam Antibiotics
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Figures

Image of FIGURE 1
FIGURE 1

Vitek System 120 (courtesy of bioMerieux).

Citation: Evangelista A, Truant A. 2002. Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria, p 413-428. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch17
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Image of FIGURE 2
FIGURE 2

Vitek 2 (courtesy of bioMerieux).

Citation: Evangelista A, Truant A. 2002. Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria, p 413-428. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch17
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Image of FIGURE 3
FIGURE 3

Vitek 2 cards (courtesy of bioMerieux).

Citation: Evangelista A, Truant A. 2002. Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria, p 413-428. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch17
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Image of FIGURE 4
FIGURE 4

MicroScan WalkAway SI (courtesy of Dade Behring).

Citation: Evangelista A, Truant A. 2002. Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria, p 413-428. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch17
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Image of FIGURE 5
FIGURE 5

MicroScan Combo ID/MIC panel (courtesy of Dade Behring).

Citation: Evangelista A, Truant A. 2002. Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria, p 413-428. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch17
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Image of FIGURE 6
FIGURE 6

Sensititre ARIS (courtesy of Trek Diagnostic Systems).

Citation: Evangelista A, Truant A. 2002. Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria, p 413-428. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch17
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Image of FIGURE 7
FIGURE 7

Phoenix system (courtesy of BD Diagnostic Systems).

Citation: Evangelista A, Truant A. 2002. Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria, p 413-428. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch17
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Image of FIGURE 8
FIGURE 8

Phoenix ID/AST panel (courtesy of BD Diagnostic Systems).

Citation: Evangelista A, Truant A. 2002. Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria, p 413-428. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch17
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Image of FIGURE 9
FIGURE 9

Etest (courtesy of AB Biodisk and Remel).

Citation: Evangelista A, Truant A. 2002. Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria, p 413-428. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch17
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References

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Tables

Generic image for table
TABLE 1.

Comparison of automated AST systems

Citation: Evangelista A, Truant A. 2002. Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria, p 413-428. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch17
Generic image for table
TABLE 2.

Semiautomated and manual AST systems

Citation: Evangelista A, Truant A. 2002. Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria, p 413-428. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch17
Generic image for table
TABLE 3.

Comparison of MIC methods for testing

Citation: Evangelista A, Truant A. 2002. Rapid Systems and Instruments for Antimicrobial Susceptibility Testing of Bacteria, p 413-428. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch17

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