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Chapter 10 : Changes in the Locus of Serogroup 1 Strains Result in Different Lipopolysaccharides Recognized by Monoclonal Antibodies but Do Not Influence Virulence

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Changes in the Locus of Serogroup 1 Strains Result in Different Lipopolysaccharides Recognized by Monoclonal Antibodies but Do Not Influence Virulence, Page 1 of 2

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Abstract:

It is well established that is an important cause of nosocomial and community-acquired pneumonia. serogroup 1, the most prevalent serogroup, can be divided into several subtypes by using monoclonal antibodies (MAb). Researchers showed recently that changes in the lipopolysaccharide (LPS) MAb binding patterns are associated with changes in the virulence properties of . Several studies showed that a majority of clinical isolates, especially strains associated with outbreaks, carried an LPS epitope that reacts with MAb 2 of the international standard panel and the MAb 3/1. Researchers used genetic fingerprinting to investigate several serogroup 1 strains originated from the same source that differed in their reactivity with MAb 3/1 but were indistinguishable or very similar. Furthermore, researchers investigated whether the loss of the reactivity with MAb 3/1 resulted in differences in the multiplication in and macrophage cells. The gene was amplified from chromosomal DNA and sequenced using an ABI 377 sequencer.

Citation: Lück P, Schuppler M, Helbig J. 2002. Changes in the Locus of Serogroup 1 Strains Result in Different Lipopolysaccharides Recognized by Monoclonal Antibodies but Do Not Influence Virulence, p 52-55. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch10

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Pulsed-Field Gel Electrophoresis
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Chromosomal DNA
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References

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Tables

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TABLE 1

Characterization of the locus in serogroup 1 strains originated from the same source

Citation: Lück P, Schuppler M, Helbig J. 2002. Changes in the Locus of Serogroup 1 Strains Result in Different Lipopolysaccharides Recognized by Monoclonal Antibodies but Do Not Influence Virulence, p 52-55. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch10

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