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Chapter 18 : Genetic Analysis of Intracellular Multiplication in Human and Protozoan Hosts

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Genetic Analysis of Intracellular Multiplication in Human and Protozoan Hosts, Page 1 of 2

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Abstract:

Several approaches have been taken to understand the genetic basis for intracellular multiplication by . The first was based on the properties of avirulent variants that had lost the ability to replicate inside macrophages and were also defective in preventing phagosome-lysosome fusion. Researchers reasoned that introduction of a wild-type region of the genome that restored the ability to replicate within and kill human macrophages would provide information about the genes that were defective in the variants. A genomic library of wild-type DNA was introduced to one of the avirulent variants, and complemented bacteria that regained the abilities to replicate intracellularly and kill host cells were identified with the aid of a plaque assay. The striking homology between the / genes and the IncI and genes involved in conjugal DNA transfer prompted us to examine if could conjugally transfer DNA. The current model for how the Icm/Dot complex influences the intracellular fate of postulates that there are two classes of Icm/Dot gene products. Several possible mechanisms could be used to specifically interrupt the fusion of phagosomes with lysosomes.

Citation: Segal G, Shuman H. 2002. Genetic Analysis of Intracellular Multiplication in Human and Protozoan Hosts, p 90-96. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch18

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Type IV Secretion Systems
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Figures

Image of FIGURE 1
FIGURE 1

Linkage map of the two regions. Region I contains and region II contains . Coding regions are indicated by bold arrows. The homologs from the IncI plasmid colIb-P9 are indicated under the corresponding gene. Shading indicates the predicted location of the protein in the bacterial cell: , lipoprotein; , cytoplasm; inner membrane; periplasm. The star indicates genes that were found to have homologs in .

Citation: Segal G, Shuman H. 2002. Genetic Analysis of Intracellular Multiplication in Human and Protozoan Hosts, p 90-96. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch18
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References

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10. Segal, G.,, and H. A. Shuman. 1998. Intracellular multiplication and human macrophage killing by Legionella pneumophila are inhibited by conjugal components of IncQ plasmid RSF1010. Mol. Miaobiol. 30:197208.
11. Segal, G.,, and H. A. Shuman. 1999. Legionella pneumophila utilizes the same genes to multiply within Acanthamoeba castellanii and human macrophages. Infect. Immun. 67:21172124.
12. Vogel, J. P.,, H. L. Andrews,, S. K. Wong,, and R. R. Isberg. 1998. Conjugative transfer by the virulence system of Legionella pneumophila. Science 279:873876.
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14. Winans, S. C.,, D. L. Bums,, and P.J. Christie. 1996. Adaptation of the conjugal transfer system for the export of pathogenic macromolecules. Trends Microbiol. 4:6468.

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