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Chapter 20 : Conjugal Transfer of Chromosomal DNA in

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Conjugal Transfer of Chromosomal DNA in , Page 1 of 2

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Abstract:

This chapter examines whether a chromosomal conjugation system exists in . Mating experiments between K6 (approximately 2.6 X 10 CFU) and Chicago-2S (approximately 8.5 X 10 CFU) typically yielded 10 Km Sm LacZ recombinants. All recombinants tested (100/100) belonged to serogroup 6, which is the same serogroup as strain Chicago-2S. This result suggested that the donor was strain K6 and the recipient was strain Chicago-2S. The mechanism of the DNA transfer was most consistent with conjugal transfer. To genetically examine the DNA transferred from K6 to Chicago-2S, genotyping by arbitrarily primed PCR (AP-PCR) and pulsed-field gel electrophoresis (PFGE) were performed among strains K6, Chicago-2S, and their transconjugants. The restriction fragment patterns of the transconjugants were distinct from the pattern exhibited by K6 and were similar to that exhibited by Chicago-2S. The transfer of DNA from the chromosome of strain K6 to Chicago-2S could occur by a variety of mechanisms. Transposition would presumably transfer only the transposition sequences to random sites that would differ in different recombinants. Researchers showed that a thymine auxotroph of was repaired by plasmid R68.45-mediated chromosomal mobilization of a prototrophic donor strain. Therefore, if had acquired an T by integration of these conjugative elements, it would become selfmobilizable like the Hfr of .

Citation: Miyamoto H, Taniguchi H, Ishimatsu S, Yoshida S, Shuman H. 2002. Conjugal Transfer of Chromosomal DNA in , p 105-108. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch20

Key Concept Ranking

Chromosomal DNA
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Pulsed-Field Gel Electrophoresis
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Figures

Image of FIGURE 1
FIGURE 1

AP-PCR DNA fingerprints of strains. Lanes: M, 100-bp ladder (Pharmacia Biotech) as a DNA size standard; 1, K6; 2, Chicago-2S; 3, HM1011; 4, HM1012; 5, HM1013; 6, HM1014; 7, HM1042; 8, HM1043.

Citation: Miyamoto H, Taniguchi H, Ishimatsu S, Yoshida S, Shuman H. 2002. Conjugal Transfer of Chromosomal DNA in , p 105-108. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch20
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Image of FIGURE 2
FIGURE 2

Cleavage patterns of I-digested genomic DNA of strains by PFGE. Lanes 1 to 8 show the patterns of the strains described in Fig. 1 . The arrowhead indicates a band that is not observed in lanes 1 and 2. Numbers to the right of the gel represent the size of DNA in kilobases.

Citation: Miyamoto H, Taniguchi H, Ishimatsu S, Yoshida S, Shuman H. 2002. Conjugal Transfer of Chromosomal DNA in , p 105-108. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch20
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Image of FIGURE 3
FIGURE 3

Southern blot analysis of I-digested genomic D N A separated by PFGE. Plasmid pLAW330 digested with dIII, which does not cut with in Tn903dII, was labeled with digoxigenin and used as a probe. Lanes 1 to 8 show the patterns of strains described in Fig. 1 . Numbers to the right of the blot represent the size of DNA in kilobases.

Citation: Miyamoto H, Taniguchi H, Ishimatsu S, Yoshida S, Shuman H. 2002. Conjugal Transfer of Chromosomal DNA in , p 105-108. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch20
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References

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1. Chen, G. C. C.,, A. Brown,, and M. W. Letna. 1986. Restriction endonuclease activities in the legionellae. Can. J. Microbiol. 32:591593.
2. Dreyfus, L. A.,, and B. B. Iglewski. 1985. Conjugation-mediated genetic exchange in Legionella pneumophila. J. Bacteriol. 161:8084.
3. knudson, G. B.,, and P. Mikesell. 1980. A plasmid in Legionella pneumophila. Infect. Immun. 29:10921095.
4. Lopez, F. de. 1993. Cloning, mapping and conjugal mobility of pLPG36, a common plasmid from Legionella pneumophila serogroup-1. J. Gen. Microbiol. 139:31713175.
5. Low, K. B., 1987. Hfr strains of Escherichia coli K-12, p. 11341137. In F. C. Neidhardt (ed.), Escherichia coli and Salmonella typhimurium. American Society for Microbiology, Washington, D.C.
6. Mintz, C. S.,, B. S. Fields,, and C.-H. Zou. 1992. Isolation and characterization of a conjugative plasmid from Legionella pneumophila. J. Gen. Microbiol. 138:13791386.
7. Scott, J. R.,, and G. G. Churchward. 1995. Conjugative transposition. Annu. Rev. Microbiol. 49:367397.
8. Smith, G. R. 1991. Conjugational recombination in E. coli: myth and mechanisms. Cell 64: 1927.
9. Tully, M. 1991. A plasmid from a virulent strain of Legionella pneumophila confers resistance to ultraviolet light. FEMS Microbiol. Lett. 90:4348.
10. Van Belkum, A.,, M. Struelens,, and W. Quint. 1993. Typing of Legionella pneumophila strains by polymerase chain reaction-mediated DNA fingerprinting. J. Clin. Microbiol. 31:21982200.
11. Wiater, L. A.,, A. B. Sadosky,, and H. A. Shaman. 1994. Mutagenesis of Legionella pneumophila using Tn903 dIIlarZ: identification of a growth-phase-regulated pigmentation gene. Mol. Microbiol. 11:641653.
12. Willetts, N.,, and R. Skurray,. 1987. Structure and function of the F factor and mechanism of conjugation, p. 11101133. In F. C. Neidhardt (ed.), Escherichia coli and Salmonella typhimurium. American Society for Microbiology, Washington, D.C.

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