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Chapter 24 : Protection against Lethal Challenge by in A/J Mice Following Immunization with Flagella

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Protection against Lethal Challenge by in A/J Mice Following Immunization with Flagella, Page 1 of 2

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Abstract:

This chapter talks about the role of the flagellum in the observed protection from legionella infection that was investigated by the authors. First, the minimal lethal dose (MLD) of two different strains of was determined by infecting intraperitoneally (i.p.) female A/J mice, aged 4 to 6 weeks, with different doses of an serogroup 6 strain of human origin (VIR+), or from the serogroup 1 Corby strain. A flagellar preparation was performed from a 6-day culture of the flagellated VIR+ strain on buffered charcoal-yeast extract (BCYE; Oxoid, Italy) agar plates at 36№C, in humidified atmosphere and 2.5% CO. Mice were observed for signs of illness and for survival for 15 days. In flagella-immunized mice, no mortality was observed, compared with 100% mortality of similarly challenged mice immunized with the sham-flagellar preparation or unimmunized control mice. Involvement of humoral immune responses in the protective effect due to flagellar immunization was investigated, also considering that flagella are present in most species and serogroups, and their composition is common in all of them.

Citation: Ricci M, Torosantucci A, Chiani P, Pinto A, Pastoris M, Baldassarri L. 2002. Protection against Lethal Challenge by in A/J Mice Following Immunization with Flagella, p 129-132. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch24

Key Concept Ranking

Legionella pneumophila
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Humoral Immune Response
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Figures

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FIGURE 1

Survival of A/J mice treated with murine or rabbit immunesera after challenge with VIR+ strain. ♦, mouse antiflagellum serum; ■, mouse nonimmune serum; ▲, rabbit antiflagellum serum; ·, rabbit nonimmune serum; *, sterile, pyrogen-free saline.

Citation: Ricci M, Torosantucci A, Chiani P, Pinto A, Pastoris M, Baldassarri L. 2002. Protection against Lethal Challenge by in A/J Mice Following Immunization with Flagella, p 129-132. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch24
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References

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1. Baldassarri, L.,, G. Donelli,, A. Gelosia,, M. C. Voglino,, A. W. Simpson,, and G. D. Christensen. 1996. Purification and characterization of the staphylococcal sHme-associated antigen and its occurrence among Staphylococcus epidermidis clinical isolates. Infect. Immun. 177: 24602468.
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Tables

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TABLE 1

Mortality of A/J mice immunized with flagellar or sham-flagellar preparations after a lethal challenge with virulent

Citation: Ricci M, Torosantucci A, Chiani P, Pinto A, Pastoris M, Baldassarri L. 2002. Protection against Lethal Challenge by in A/J Mice Following Immunization with Flagella, p 129-132. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch24

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